动物营养学报 >

2018 , Vol. 30 >Issue 8: 3043 - 3054

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.08.021

反刍动物营养 Ruminant nutrition

补喂瘤胃保护性色氨酸对绵羊血浆色氨酸及部分相关代谢物含量的影响

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  • 新疆农业大学动物科学学院, 新疆肉乳用草食动物营养重点实验室, 乌鲁木齐 830052
王根(1983-),男,山东菏泽人,硕士研究生,研究方向为反刍动物营养。E-mail:570610080@qq.com

收稿日期: 2018-01-29

  网络出版日期: 2018-08-18

基金资助

国家自然科学基金（31760681）

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Effects of Rumen Protected Tryptophan Supplementation on Plasma Contents of Tryptophan and Related Metabolites of Sheep

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  • Xinjiang Key Laboratory of Meat & Milk Production Herbivore Nutrition, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China

Received date: 2018-01-29

  Online published: 2018-08-18

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摘要

本试验旨在探究饲粮中添加瘤胃保护性色氨酸（RPTrp）对绵羊血浆色氨酸（Trp）及相关代谢物含量的影响。试验选取年龄（3.0±0.5）岁、平均体重（53.49±2.41）kg、健康的萨福克绵羊15只，随机分为3组，每组5只，分别为对照组和试验Ⅰ、Ⅱ组，精料饲喂量为10 g/（kg BW·d），玉米青贮为1.8 kg/d，混合干草自由采食，在此基础上，试验Ⅰ、Ⅱ组绵羊分别添喂150 mg/（kg BW·d）Trp和333 mg/（kg BW·d）RPTrp，进行25 d的饲养试验。结果表明：1）上午和下午饲喂后2 h，试验Ⅰ组血浆总色氨酸（T-Trp）、游离色氨酸（F-Trp）含量极显著高于对照组（P<0.01），血浆犬尿氨酸（Kyn）含量显著高于对照组（P<0.05）；上午饲喂后6 h，试验Ⅱ组血浆T-Trp、F-Trp含量显著高于对照组和试验Ⅰ组（P<0.05）；下午饲喂后4 h，试验组血浆F-Trp含量显著高于对照组（P<0.05）。2）上午饲喂后10 h、下午饲喂后8 h，试验Ⅱ组血浆5-羟色胺（5-HT）含量显著高于对照组（P<0.05）；上午饲喂后6、8 h，试验组血浆褪黑素（ML）含量极显著高于对照组（P<0.01），试验组间无显著性差异（P>0.05），下午饲喂后4、8 h，试验Ⅱ组显著高于对照组（P<0.05）。3）下午饲喂后2 h，试验组血浆游离脂肪酸（FFA）含量极显著低于对照组（P<0.01）；各时间点各组间血浆白蛋白（ALB）含量无显著差异（P>0.05）。4）与对照组相比，试验组可以极显著提高血浆谷胱甘肽过氧化物酶（GSH-Px）活性（P<0.05），并极显著降低血浆丙二醛（MDA）含量（P<0.01），试验Ⅱ组还可极显著提高血浆总抗氧化能力（T-AOC）（P<0.01）。因此，补喂Trp可使绵羊采食后血浆T-Trp、F-Trp和Kyn含量迅速升高，而补喂RPTrp时上述作用则较平缓。补喂Trp、RPTrp对绵羊血浆ALB、FFA、5-HT含量影响较小，仅有个别时间点作用显著。补喂Trp、RPTrp使绵羊白天血浆中ML含量升高，补喂Trp对夜间绵羊血浆中ML含量没有显著影响，补喂RPTrp可使绵羊夜间血浆ML含量维持在相对较高的水平上。补喂Trp、RPTrp可提高绵羊血浆抗氧化能力。

关键词： 绵羊; 瘤胃保护性色氨酸; 游离色氨酸; 犬尿氨酸; 5-羟色胺; 褪黑素

本文引用格式

王根, 赵芳, 高超, 赵国栋, 李晓斌, 马晨, 杨开伦 . 补喂瘤胃保护性色氨酸对绵羊血浆色氨酸及部分相关代谢物含量的影响[J]. 动物营养学报, 2018 , 30(8) : 3043 -3054 . DOI: 10.3969/j.issn.1006-267x.2018.08.021

Abstract

This study aimed to investigate the effects of rumen protected tryptophan supplementation on plasma contents of tryptophan and related metabolites of sheep. Fifteen healthy Suffolk sheep aged (3.0±0.5) years with average body weight of (53.49±2.41) kg were randomly divided into 3 groups (5 sheep in each group), which were control group, trail group Ⅰ and trial group Ⅱ. All sheep were fed 10 g/(kg BW·d) concentrate, 1.8 kg/d corn silage, and allowed free choice continuous access to mixed hay. Animals of trail group Ⅰ and trial group Ⅱ were supplemented with 150 mg/(kg BW·d) tryptophan, 333 mg/(kg BW·d) rumen protected tryptophan, respectively. The feeding trial lasted for 25 d. The results showed as follows:1)at 2 hours after feeding in the morning and the afternoon, compared with control group, the contents of plasma total tryptophan and free tryptophan in trial group Ⅰ were significantly increased (P<0.01), and the content of plasma kynurenine was significantly increased (P<0.05); at 6 hours after feeding in the morning, the content of plasma total tryptophan and free tryptophan in trial group Ⅱ were significantly higher than those in control group and trial group Ⅰ (P<0.05); at 4 hours after feeding in the afternoon, the content of plasma free tryptophan in trial groups was significantly higher than that in control group (P<0.05). 2) At 10 hours after feeding in the morning and 8 hours after feeding in the afternoon, the content of plasma 5-hydroxytryptamine in trial group Ⅱ was significantly higher than that in control group (P<0.05); at 6 and 8 hours after feeding in the morning, the content of plasma melatonin in trial groups were significantly higher than that in control group (P<0.01), and there was no significant difference between trial groups (P>0.05); at 4 and 8 hours after feeding in the afternoon, the content of plasma melatonin in trial group Ⅱ was significantly higher than that in control group (P<0.05). 3)At 2 hours after feeding in the afternoon, the content of plasma free fatty acids in trial groups was significantly lower than that in control group (P<0.01); there were no significant differences in plasma albumin content among groups at different time points (P>0.05). 4) Compared with control group, trial groups had significantly higher glutathione peroxidase (GSH-Px) activity and significantly lower malonaldehyde (MDA) content in plasma (P<0.01), and trial group Ⅱ had significantly higher total antioxidant capacity in plasma (P<0.01). Therefore, the supplementation of tryptophan can rapidly increase the contents of total tryptophan, free tryptophan and kynurenine in plasma of sheep after feeding, while the above effects of rumen protected tryptophan are more moderate. The supplementations of tryptophan and rumen protected tryptophan have tiny effects on the contents of plasma albumin, free fatty acids and 5-hydroxytryptamine, and significant differences only appears at a few time points. The supplementations of tryptophan and rumen protected tryptophan can increase the content of plasma melatonin during daytime, that of tryptophan has no significant effect on the content of plasma melatonin during nighttime, while that of rumen protected tryptophan can maintain the content of plasma melatonin at a high level during nighttime. Plasma antioxidant capacity is improved by the supplementations of tryptophan and rumen protected tryptophan.

Key words： sheep; rumen protected tryptophan; free tryptophan; kynurenine; 5-hydroxytryptamine; melatonin

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