动物营养学报 >

2018 , Vol. 30 >Issue 8: 3063 - 3072

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.08.023

水产营养 Aquaculture Nutrition

不同铬源对喂食高葡萄糖饲料鲤生长性能、血清生化指标及肝胰脏糖代谢酶活性的影响

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  • 1. 宁波大学海洋学院鱼类营养研究室, 宁波 315211;
    2. 天津农学院水产学院, 天津市水产生态及养殖重点实验室, 天津 300384
崔培(1985-),女,天津人,博士研究生,从事水产动物营养与饲料学研究。E-mail:icp7410@hotmail.com

收稿日期: 2018-01-15

  网络出版日期: 2018-08-18

基金资助

浙江省重中之重一级学科（水产）开放基金（xkzsc1501）；天津市自然科学基金项目（14JCQNJC15100）；农业部热带亚热带水产资源利用与养殖重点实验室开放基金

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Effects of Different Chromium Sources on Growth Performance, Serum Biochemical Indices and Hepatopancreas Glycometabolism Enzyme Activities of Common Carp Fed High Glucose Diets

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  • 1. Laboratory of Fish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China;
    2. Tianjin Key Laboratory of Aqua-Ecology and Aquaculture, Fisheries Science Department, Tianjin Agricultural University, Tianjin 300384, China

Received date: 2018-01-15

  Online published: 2018-08-18

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摘要

本试验旨在研究饲料中不同铬源对喂食高葡萄糖饲料鲤生长性能、血清生化指标及肝胰脏糖代谢酶活性的影响。配制4种纯化饲料，分别为不添加铬的基础饲料（对照组）及基础饲料中分别添加三氧化二铬（Cr2O3）、吡啶羧酸铬（CrPic）和蛋氨酸铬（CrMet）的试验饲料，试验饲料中铬添加水平在2.60 mg/kg（以三价铬离子计）左右。选择初始体重为（40.95±4.80）g的鲤720尾，随机分为4组，每组设3个重复，每个重复60尾。每组饲喂1种饲料，饲喂基础饲料的为对照组。养殖周期为60 d。结果表明：与对照组相比，添加CrPic、CrMet能显著提高鲤的增重率（WGR）、特定生长率（SGR）、饲料效率（FE）以及蛋白质效率（PER）（P<0.05）；添加Cr2O3能显著提高鲤的WGR、SGR（P<0.05）；添加3种铬源对全鱼水分、灰分、蛋白质含量均无显著影响（P>0.05），添加CrPic和CrMet能显著提高全鱼脂肪含量（P<0.05）；添加3种铬源均能显著提高肌糖原含量（P<0.05），而肝糖原含量仅在CrMet组得到显著提高（P<0.05）；添加CrMet显著降低了血清甘油三酯（TG）和总胆固醇（TC）含量（P<0.05）；添加CrMet显著提高了鱼体胰岛素（INS）、胰岛素受体（ISR）、生长激素（GH）含量以及乳酸脱氢酶（LDH）和肌酸激酶（CK）活性（P<0.05），显著降低了血清葡萄糖和皮质醇（COR）含量（P<0.05）；添加3种铬源均显著提高了肝胰脏糖酵解途径丙酮酸激酶（PK）、己糖激酶（HK）活性（P<0.05），添加CrPic、CrMet能显著降低肝胰脏糖异生途径磷酸烯醇式丙酮酸激酶（PEPCK）活性（P<0.05）。综合得出，对于喂食高葡萄糖饲料的鲤而言，CrMet在促进生长，提高饲料利用以及糖利用能力方面具有最明显的效果，CrPic次之，而Cr2O3最差。

关键词： 铬; 生长性能; 血清生化指标; 糖代谢酶活性; 鲤

本文引用格式

崔培, 孙金辉, 尹帅, 程镇燕, 乔秀亭, 周歧存 . 不同铬源对喂食高葡萄糖饲料鲤生长性能、血清生化指标及肝胰脏糖代谢酶活性的影响[J]. 动物营养学报, 2018 , 30(8) : 3063 -3072 . DOI: 10.3969/j.issn.1006-267x.2018.08.023

Abstract

A 60-day feeding trial was conducted to evaluate the effects of different chromium sources on growth performance, serum biochemical indices and hepatopancreas glycometabolism enzyme activities of common carp fed high glucose diets. Four purification diets were formulated including a basal diet without chromium supplementation (control group) and three experimental diets[the basal diet supplemented with chromic oxide (Cr2O3), chromium pyridine carboxylate (CrPic) and methionine chromium (CrMet), respectively], and chromium supplemental level[calculated as trivalent chromic ion (Cr3+)] in experimental diets was about 2.60 mg/kg, respectively, and the basal diet (without chromium). A total of 720 common carps with initial body weight of (40.95±4.80) g were selected and randomly divided into four groups with 3 replicates per group and 60 carps per replicate. Each diet was fed to one of the groups, and the group fed the basal diet was control group. The results showed as follows:compared with control group, the supplementations of CrMet and CrPic significantly increased weight gain rate (WGR), specific growth rate (SGR), protein efficiency ratio (PER) and feed efficiency (FE) (P<0.05); the supplementation of Cr2O3 significantly increased WGR and SGR (P<0.05); the supplementations of three sources of chromium had no significant effects on content of moisture, protein and ash of whole body (P<0.05), while lipid content of whole body was significantly increased by the supplementations of CrPic and CrMet (P<0.05); muscle glycogen content was significantly increased by the supplementations of that were fed the basal diet were lower than those given the other diets (P>0.05), and hepatic glycogen content was significantly increased by the supplementation of CrMet (P<0.05); serum cholesterol (TC) and triglyceride (TG) contents were significantly decreased by the supplementation of CrMet (P<0.05); the contents of insulin (INS), insulin receptor (ISR) and growth hormone (GH), and the activities of lactate dehydrogenase (LDH) and creatine kinase (CK) in serum were significantly increased by the supplementation of CrMet (P<0.05), while the contents of glucose and cortisol (COR) in serum were significantly decreased (P<0.05); the supplementations of three sources of chromium significantly increased the activities of hexokinase (HK) and pyruvate kinase (PK) in embden-meyerhof-parnas pathway (P<0.05), but significantly decreased the activities of phosphoenolpyruvate carboxykinase (PEPCK) activities in glyconeogenesis pathway in hepatopancreas (P<0.05). In conclusion, dietary supplementation of CrMet has the most obvious effects on promoting growth, improving feed utilization and glucose utilization of common carp fed high glucose, the next in order is CrPic, and the effects of Cr2O3 are not so good.

Key words： chromium; growth performance; serum biochemical indices; glycometabolism enzyme activity; common carp

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