水产营养 Aquaculture Nutrition

氨氮胁迫下饥饿和再投喂对黄颡鱼幼鱼生长性能、血液健康、抗氧化能力及免疫应答的影响

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  • 宁波大学海洋学院, 宁波 315211
谢雨欣(1995-),女,湖北荆州人,硕士研究生,从事水生动物营养与免疫研究。E-mail:279061347@qq.com

收稿日期: 2018-01-18

  网络出版日期: 2018-08-18

基金资助

国家自然科学基金项目(31472279,31502176);宁波市自然科学基金项目(2016A610083)

Effects of Starvation and Refeeding on Growth Performance, Blood Health, Antioxidant Capacity and Immune Response of Juvenile Yellow Catfish under Ammonia Stress

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  • School of Marine Sciences, Ningbo University, Ningbo 315211, China

Received date: 2018-01-18

  Online published: 2018-08-18

摘要

为了研究氨氮胁迫下饥饿和再投喂对黄颡鱼幼鱼生长性能、血液健康、抗氧化能力及免疫应答的影响,以初始体质量为(14.36±0.21)g的黄颡鱼幼鱼为研究对象,随机分为对照组和试验组(每组3个重复,每个重复30尾),对照组人工饱食投喂42 d,试验组饥饿14 d后恢复饱食投喂28 d,2组试验鱼都暴露于5.7 mg/L总氨氮中。结果显示:氨氮胁迫下饥饿14 d后,试验组黄颡鱼幼鱼体质量、头肾巨噬细胞吞噬指数和血清溶菌酶活性均显著低于对照组(P<0.05);试验组黄颡鱼幼鱼血清谷丙转氨酶、谷草转氨酶活性和尿酸含量,肝脏超氧化物歧化酶活性和丙二醛含量均显著高于对照组(P<0.05)。氨氮胁迫下饥饿14 d再恢复投喂28 d后,试验组黄颡鱼幼鱼终末体质量显著低于对照组(P<0.05),但特定生长率显著高于对照组(P<0.05);试验组黄颡鱼幼鱼血清谷丙转氨酶、谷草转氨酶、碱性磷酸酶活性及尿酸和甘油三酯含量均显著低于对照组(P<0.05);试验组黄颡鱼幼鱼肝脏超氧化物歧化酶活性与对照组无显著性差异(P>0.05);试验组黄颡鱼幼鱼头肾巨噬细胞吞噬指数和血清总免疫球蛋白含量显著高于对照组(P<0.05);试验组黄颡鱼幼鱼头肾巨噬细胞呼吸爆发、血清总补体含量和溶菌酶活性与对照组无显著性差异(P>0.05)。结果表明,氨氮胁迫下,饥饿会对黄颡鱼幼鱼的生长及健康造成抑制;饥饿后再投喂,黄颡鱼幼鱼表现出部分生长补偿,血液恶化、抗氧化酶活性和免疫抑制得到不同程度的缓解。

本文引用格式

谢雨欣, 张木子, 黎明, 袁莉霞, 李冰, 陈雨诗, 王日昕 . 氨氮胁迫下饥饿和再投喂对黄颡鱼幼鱼生长性能、血液健康、抗氧化能力及免疫应答的影响[J]. 动物营养学报, 2018 , 30(8) : 3073 -3081 . DOI: 10.3969/j.issn.1006-267x.2018.08.024

Abstract

This experiment was conducted to study the effects of starvation and refeeding on growth performance, blood health, antioxidant capacity and immune response of juvenile yellow catfish (Pelteobagrus fulvidraco) under ammonia stress. Juvenile yellow catfish with the initial body weight of (14.36±0.21) g were randomly distributed into two groups (control group and experimental group) with three replicates per group and 30 fish per replicate. The fish in control group were fed to satiation for 42 days, and the fish in experimental group were starved for 14 days, then refeeding to satiation for 28 days. All fish were exposed to 5.70 mg/L total ammonia nitrogen. The results showed as follows:after starvation for 14 days under ammonia stress, the final body weight, head-kidney macrophage phagocytic index and serum lysozyme activity of fish in the experimental group were significantly lower than those of fish in the control group (P<0.05), and the experimental group showed significantly higher glutamic-pyruvic transaminase and glutamic oxalacetic transaminase activities and uric acid content in serum, superoxide dismutase activity and malondialdehyde contents in liver compared with the control group (P<0.05). After starvation for 14 days and then refeeding for 28 days under ammonia stress, the final body weight, serum glutamic-pyruvic transaminase, glutamic oxalacetic transaminase and alkaline phosphatase activities, uric acid and triglycerides contents of fish in the experimental group were significantly lower than those of fish in the control group (P<0.05), but the experimental group showed significantly higher specific growth rate, head-kidney macrophage phagocytic index and serum total immunoglobulin content compared with the control group (P<0.05). No significant differences between experimental and control groups were identified in liver superoxide dismutase activity, serum lysozyme activity and total complement content, and head-kidney macrophage respiratory burst (P>0.05). The results indicate that starvation exerts its adverse effects by interfering with growth and health of juvenile yellow catfish under ammonia stress, but the blood degradation and the suppression of antioxidant enzyme activities and immune of juvenile yellow catfish can be mitigated through manipulation of refeeding, and the fish have partially compensatory growth.

参考文献

[1] 农业部渔业渔政管理局.中国渔业统计年鉴[M].北京:中国农业出版社,2017.

[2] BENLI A C K,KÖKSAL G,ÖZKUL A.Sublethal ammonia exposure of Nile tilapia (Oreochromis niloticus L.):effects on gill,liver and kidney histology[J].Chemosphere,2008,72(9):1355-1358.  

[3] CHO S H,LEE S M,PARK B H,et al.Compensatory growth of juvenile olive flounder,Paralichthys olivaceus L.,and changes in proximate composition and body condition indexes during fasting and after refeeding in summer season[J].Journal of the World Aquaculture Society,2006,37(2):168-174.  

[4] OH S Y,NOH C H,KANG R S,et al.Compensatory growth and body composition of juvenile black rockfish Sebastes schlegeli following feed deprivation[J].Fisheries Science,2008,74(4):846-852.  

[5] HAYWARD R S,NOLTIE D B,WANG N.Use of compensatory growth to double hybrid sunfish growth rates[J].Transactions of the American Fisheries Society,1997,126(2):316-322.  

[6] XIE S,ZHU X,CUI Y,et al.Compensatory growth in the gibel carp following feed deprivation:temporal patterns in growth,nutrient deposition,feed intake and body composition[J].Journal of Fish Biology,2001,58(4):999-1009.  

[7] ZHU X M,XIE S Q,LEI W,et al.Compensatory growth in the Chinese long snout catfish,Leiocassis longirostris following feed deprivation:temporal patterns in growth,nutrient deposition,feed intake and body composition[J].Aquaculture,2005,248(1/2/3/4):307-314.

[8] PAUL A J,PAUL J M,SMITH R L.Compensatory growth in Alaska yellowfin sole,Pleuronectes asper,following food deprivation[J].Journal of Fish Biology,1995,46(3):442-448.  

[9] HEIDE A,FOSS A,STEFANSSON S O,et al.Compensatory growth and fillet crude composition in juvenile Atlantic halibut:effects of short term starvation periods and subsequent feeding[J].Aquaculture,2006,261(1):109-117.  

[10] 田娟,涂玮,曾令兵,等.饥饿和再投喂期间尼罗罗非鱼生长、血清生化指标和肝胰脏生长激素、类胰岛素生长因子-Ⅰ和胰岛素mRNA表达丰度的变化[J].水产学报,2012,36(6):900-907.

[11] 赵忠波,汪帆,吴巧婉,等.放养密度对黄颡鱼的生长性能和养殖水体水质的影响[J].中国农学通报,2016,32(23):37-42.

[12] 黎庆,龚诗雁,黎明.慢性氨氮暴露诱发黄颡鱼幼鱼谷氨酰胺积累、氧化损伤及免疫抑制的研究[J].水产学报,2015,39(5):728-734.

[13] HEGAZI M M,ATTIA Z I,ASHOUR O A.Oxidative stress and antioxidant enzymes in liver and white muscle of Nile tilapia juveniles in chronic ammonia exposure[J].Aquatic Toxicology,2010,99(2):118-125.  

[14] JOHANSSON O,WEDBORG M.The ammonia-ammonium equilibrium in seawater at temperatures between 5 and 25℃[J].Journal of Solution Chemistry,1980,9(1):37-44.  

[15] MILLER N J,RICE-EVANS C,DAVIES M J,et al.A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates[J].Clinical Science,1993,84(4):407-412.  

[16] BEAUCHAMP C,FRIDOVICH I.Superoxide dismutase:improved assays and an assay applicable to acrylamide gels[J].Analytical Biochemistry,1971,44(1):276-287.  

[17] AEBI H.Catalase in vitro[J].Methods in Enzymology,1984,105:121-126.

[18] BUEGE J A,AUST S D.Microsomal lipid peroxidation[J].Methods in Enzymology,1978,52:302-310.

[19] HULTMARK D,STEINER H,RASMUSON T,et al.Insect immunity.Purification and properties of three inducible bactericidal proteins from hemolymph of immunized pupae of Hyalophora cecropia[J].European Journal of Biochemistry,1980,106(1):7-16.

[20] WU J,SHANG H.Clinical immunology and inspection[M]//YE Y,WANG Y,SHEN Z.National Guide to Clinical Laboratory Procedures.Nanjing:Southeast University Press,2006:595-606.

[21] PULSFORD A L,CRAMPE M,LANGSTON A,et al.Modulatory effects of disease,stress,copper,TBT and vitamin E on the immune system of flatfish[J].Fish & Shellfish Immunology,1995,5(8):631-643.  

[22] STOLEN J S,FLETCHER T C,ANDERSON D R,et al.Techniques in fish immunology[M].Fair Haven,NJ:SOS Publications,1990.

[23] 高露姣,陈立侨,宋兵.饥饿和补偿生长对史氏鲟幼鱼摄食、生长和体成分的影响[J].水产学报,2004,28(3):279-284.

[24] FALAHATKAR B,AKHAVAN S R,EFATPANAH I,et al.Effect of winter feeding and starvation on the growth performance of young-of-year (YOY) great sturgeon,Huso huso[J].Journal of Applied Ichthyology,2013,29(1):26-30.  

[25] SEVGILI H,HO?SU B,EMRE Y,et al.Compensatory growth after various levels of dietary protein restriction in rainbow trout,Oncorhynchus mykiss[J].Aquaculture,2012,344-349:126-134.

[26] GAMBARDELLA C,GALLUS L,AMAROLI A,et al.Fasting and re-feeding impact on leptin and aquaglyceroporin 9 in the liver of European sea bass (Dicentrarchus labrax)[J].Aquaculture,2012,354-355:1-6.

[27] ALI T E S,MARTÍNEZ-LLORENS S,MOÑINO A V,et al.Effects of weekly feeding frequency and previous ration restriction on the compensatory growth and body composition of Nile tilapia fingerlings[J].The Egyptian Journal of Aquatic Research,2016,42(3):357-363.  

[28] 姚峰,甄恕其,何爱华,等.初始体重差异对黄颡鱼补偿生长的影响[J].淡水渔业,2008,38(5):65-69.

[29] 杨严鸥,姚峰.间歇性饥饿对瓦氏黄颡鱼生长及肝脏抗氧化功能的影响[J].中国饲料,2011(21):31-34.

[30] LI M,GONG S Y,LI Q,et al.Ammonia toxicity induces glutamine accumulation,oxidative stress and immunosuppression in juvenile yellow catfish Pelteobagrus fulvidraco[J].Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology,2016,183/184:1-6.

[31] GAO Y,YI Y Y,WU H Z,et al.Molecular cloning and characterization of secretory and membrane-bound IgM of turbot[J].Fish & Shellfish Immunology,2014,40(2):354-361.  

[32] 施兆鸿,彭士明,宋国,等.饥饿与再投喂对条石鲷幼鱼组织和血清中主要代谢酶活性及糖元含量的影响[J].水产学报,2012,36(9):1435-1442.

[33] 税春,施永海,徐嘉波,等.饥饿及恢复投喂对菊黄东方鲀生长和血清生化的影响[J].水产科技情报,2017,44(2):57-61.

[34] FENG G P,SHI X T,HUANG X R,et al.Oxidative stress and antioxidant defenses after long-term fasting in blood of Chinese sturgeon (Acipenser sinensis)[J].Procedia Environmental Sciences,2011,8:469-475.

[35] YENGKOKPAM S,DEBNATH D,PAL A K,et al.Short-term periodic feed deprivation in Labeo rohita fingerlings:effect on the activities of digestive,metabolic and anti-oxidative enzymes[J].Aquaculture,2013,412/413:186-192.

[36] LI S,TAN H Y,WANG N,et al.The role of oxidative stress and antioxidants in liver diseases[J].International Journal of Molecular Sciences,2015,16(12):26087-26124.

[37] SUN H J,WANG W Q,LI J J,et al.Growth,oxidative stress responses,and gene transcription of juvenile bighead carp (Hypophthalmichthys nobilis) under chronic-term exposure of ammonia[J].Environmental Toxicology and Chemistry,2014,33(8):1726-1731.  

[38] MAGNADOTTIR B.Immunological control of fish diseases[J].Marine Biotechnology,2010,12(4):361-379.  

[39] 楼宝,史会来,毛国民,等.饥饿及恢复投饵过程中花鲈肌肉组成及非特异免疫水平的变化[J].水产学报,2008,32(6):929-938.

[40] CARUSO G,DENARO M G,CARUSO R,et al.Response to short term starvation of growth,haematological,biochemical and non-specific immune parameters in European sea bass (Dicentrarchus labrax) and blackspot sea bream (Pagellus bogaraveo)[J].Marine Environmental Research,2011,72(1/2):46-52.

[41] BOWDEN T J.Modulation of the immune system of fish by their environment[J].Fish & Shellfish Immunology,2008,25(4):373-383.  
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