分子营养 Molecular Nutrition

基于气相色谱-质谱联用技术的急性热应激肉鸡血清物质代谢组学研究

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  • 安徽科技学院动物科学学院, 滁州 233100
贺绍君(1982-),男,河南虞城人,副教授,博士,从事畜禽应激与疾病发生研究。E-mail:shaojunhe2011@126.com

收稿日期: 2018-02-07

  网络出版日期: 2018-08-18

基金资助

国家自然科技基金青年项目(31601983,31702306);国家级星火计划面上项目(2015GA710025);安徽省科技厅自然科学研究项目(1508085QC61);安徽省教育厅自然科学重点项目(KJ2015A237)

Study on Serum Substance Metabolomics in Acutely Heat-Stressed Broilers Using Gas Chromatography-Mass Spectrometry Technique

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  • College of Animal Science, Anhui Science and Technology University, Chuzhou 233100, China

Received date: 2018-02-07

  Online published: 2018-08-18

摘要

本研究旨在运用气相色谱-质谱联用(GC-MS)技术分析急性热应激肉鸡血清物质代谢组学变化,鉴定出差异代谢物和差异代谢通路,从代谢组学层次揭示肉鸡急性热应激的营养物质代谢途径变化的发生机制。试验选择20只35日龄爱拔益加(AA)肉鸡,随机均分为2组,每组5个重复,每个重复6只鸡。对照组按常规饲养标准温度饲养,热应激组于(32±1)℃下饲养,均持续12 h。翅下静脉采血2 mL,分离血清,经衍生化处理,运用GC-MS技术获得2组肉鸡血清代谢图谱,对试验收集的数据进行代谢物主成分分析和偏最小二乘法分析,鉴定差异代谢物并进行差异代谢通路富集分析。结果表明:利用GC-MS技术,在肉鸡血清中共检测到144种代谢物,筛选出30种差异代谢物[变量权重值(VIP)>1,P<0.05]。其中延胡索酸、羟丁酸、丙氨酸等14种代谢物含量上调[差异倍数(FC)>1],草酸、苹果酸、二羟基丙酮等16种代谢物含量下调(FC<1)。代谢通路富集分析表明机体三羧酸循环、半乳糖代谢、丙酸代谢、脂肪酸生物合成等代谢通路发生显著改变(P<0.05或P<0.01)。由此可见,肉鸡发生急性热应激时血清代谢物和物质代谢途径发生显著改变。鉴定的30种差异代谢物和7种差异代谢通路可作为标志物用于揭示急性热应激的发病机理。

本文引用格式

贺绍君, 丁金雪, 李静, 胡洪, 刘德义 . 基于气相色谱-质谱联用技术的急性热应激肉鸡血清物质代谢组学研究[J]. 动物营养学报, 2018 , 30(8) : 3116 -3124 . DOI: 10.3969/j.issn.1006-267x.2018.08.029

Abstract

This experiment was conducted to investigate the changes of serum substance metabolomics in broilers during acute heat stress using gas chromatography-mass spectrometry (GC-MS) technique, and the identified differential metabolites and metabolic pathways were used to explore the metabolic mechanisms of nutrient metabolism pathway changes of acutely heat-stressed broilers. Twenty 35-day-old Arbor Acres (AA) broilers were randomly divided into two groups with 5 replicates per group and 6 broilers per replicate. The control group was fed at normal feeding standard temperature, and the heat stress group was fed at (32±1)℃, both for 12 hours. Blood samples were collected from inferior wing vein, and the separated serum was derivative, serum metabolic profiles of two groups were obtained by GC-MS technique. Analytical methods of principal component and supervised partial least squares discriminant were used to analyze the resultant data, the differential metabolites and metabolic pathways enrichment analysis were conducted. The results showed that 144 metabolites were determined in serum of broilers by GC-MS technique, thirty differential metabolites were screened out[variable important in projection (VIP)>1, P<0.05]. The contents of fourteen metabolites such as fumaric acid, oxybutyric acid and alanine were up-regulated[fold change (FC)>1], the contents of 16 metabolites such as oxalic acid, malic acid and dihydroxyacetone were down-regulated (FC<1). Through the metabolic pathway enrichment analysis, it was found that tricarboxylic acid cycle, galactose metabolism, propionate metabolism, fatty acid biosynthesis and other pathways were significant changed (P<0.05 or P<0.01). In conclusion, serum metabolites and substance metabolic pathway have significant change during acutely heat stress in broilers, and the identified 30 metabolites and 7 metabolic pathways may be used as metabolic biomarkers for the pathogenesis of acute heat stress.

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