维生素A对奶牛乳腺上皮细胞乳脂和乳蛋白合成相关基因表达的影响

苏芮, 刘阳, 闫素梅, 史彬林, 赵艳丽, 石惠宇

doi:10.3969/j.issn.1006-267x.2018.08.033

动物营养学报 >

2018 , Vol. 30 >Issue 8: 3151 - 3158

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.08.033

分子营养 Molecular Nutrition

维生素A对奶牛乳腺上皮细胞乳脂和乳蛋白合成相关基因表达的影响

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内蒙古农业大学动物科学学院, 呼和浩特 010018

苏芮(1993-),女,内蒙古乌兰察布人,硕士研究生,从事反刍动物营养研究。E-mail:654916601@qq.com

收稿日期: 2018-02-01

网络出版日期: 2018-08-18

基金资助

国家自然科学基金（31160466）

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Effects of Vitamin A on Gene Expressions Related to Milk Fat and Protein Synthesis in Bovine Mammary Epithelial Cells

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College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2018-02-01

Online published: 2018-08-18

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摘要

本试验旨在研究维生素A对奶牛乳腺上皮细胞（BMECs）内乳脂和乳蛋白合成相关基因表达的影响。采用单因素完全随机试验设计，将第3代BMECs随机分为6个处理，每个处理6个重复，使用无血清培养基饥饿24 h后，采用维生素A浓度分别为0（对照）、0.05、0.10、0.20、1.00和2.00 μg/mL的培养基培养24 h。结果表明：与对照组相比，1.00、2.00 μg/mL维生素A可以显著提高相对增殖率以及甘油三酯（TG）含量（P<0.05）；维生素A能显著提高乳脂合成相关基因过氧化酶体增殖物激活受体γ（PPARG，0.05、0.10、0.20、1.00和2.00 μg/mL）、固醇调节元件结合蛋白1（SREBP1，0.05、0.10 μg/mL）、硬脂酰辅酶A去饱和酶（SCD，0.05、0.10 μg/mL）的基因表达量（P<0.05）；维生素A也能显著提高乳蛋白合成相关基因信号转导转录激活因子5（STAT5，0.20 μg/mL）、αs1-酪蛋白（CSN1S1，0.05和0.10 μg/mL）、κ-酪蛋白（CSN3，0.10 μg/mL）基因表达量（P<0.05）；维生素A也能显著提高乳脂合成相关酶乙酰辅酶A羧化酶（ACACA）活性（0.05、0.10、0.20和1.00 μg/mL）（P<0.05）。结果提示，维生素A对BMECs内乳脂、乳蛋白合成相关基因表达的促进效果呈浓度依赖性，以0.10 μg/mL维生素A效果较好。

关键词： 维生素A; 乳脂; 乳蛋白; 基因表达

本文引用格式

苏芮, 刘阳, 闫素梅, 史彬林, 赵艳丽, 石惠宇 . 维生素A对奶牛乳腺上皮细胞乳脂和乳蛋白合成相关基因表达的影响[J]. 动物营养学报, 2018 , 30(8) : 3151 -3158 . DOI: 10.3969/j.issn.1006-267x.2018.08.033

Abstract

This experiment was conducted to investigate the effects of vitamin A on gene expresions related to milk fat and protein synthesis in bovine mammary epithelial cells (BMECs). The experiment was designed as a single factor randomized trial, and the 3rd generation BMECs were randomly divided into 6 treatments with 6 replicates per group. After cultured in culture medium without serum for 24 h, cells in different treatments were cultured in culture medium supplemented with 0(control), 0.05, 0.10, 0.20, 1.00 and 2.00 μg/mL vitamin A for 24 h, respectively. The results showed that compared with the control group, 1.00 and 2.00 μg/mL vitamin A could significantly increase relative growth rate and triacylglycerols (TG) content (P<0.05); vitamin A could significantly up-regulate expression levels of genes related to milk fat synthesis, such as peroxisome proliferator-activated receptor γ (PPARG, 0.05, 0.10, 0.20, 1.00 and 2.00 μg/mL), sterol regulatory element-binding protein 1 (SREBP1, 0.05 and 0.10 μg/mL), stearoyl-CoA desaturase (SCD, 0.05 and 0.10 μg/mL) (P<0.05); vitamin A could significantly up-regulate expression levels of genes related to milk protein synthesis, such as signal transducers and activators of transcription 5 (STAT5, 0.20 μg/mL), α-casein (CSN1S1, 0.05 and 0.10 μg/mL) and κ-casein (CSN3, 0.10 μg/mL) (P<0.05); vitamin A could significantly increase acetylcoenzyme A (ACACA) activity (0.05, 0.10, 0.20 and 1.00 μg/mL) (P<0.05), which was related to milk fat synthesis. In conclusion, vitamin A has concentration-dependent effects on expressions of genes related to milk fat and protein synthesis in BMECs, and the promotion effects of 0.10 μg/mL vitamin A are better.

Key words： vitamin A; milk fat; milk protein; gene expression

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