饲料营养 Feed science and technology

外源蛋白酶对肉鸡饲粮体外降解效率的影响

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  • 1. 湖南农业大学动物科学技术学院, 长沙 410128;
    2. 湖南领道农业环保科技有限公司, 长沙 410128
王雄(1991-),男,湖南长沙人,硕士研究生,从事饲料资源开发与利用的研究。E-mail:188735129@qq.com

收稿日期: 2018-02-05

  网络出版日期: 2018-08-18

基金资助

长沙市科技计划项目——畜禽养殖粪污一体化处理技术研究与推广示范项目(kh1703120)

Effects of Exogenous Protease on in Vitro Degradation Efficiency of Broiler Diets

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  • 1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
    2. Hunan Lingdao Agriculture Environmental Protection Technology Co., Ltd., Changsha 410128, China

Received date: 2018-02-05

  Online published: 2018-08-18

摘要

本试验旨在研究外源蛋白酶对肉鸡饲粮体外降解效率的影响,并确定蛋白酶的最适添加量,为快速评价蛋白酶有效性提供理论参考。试验采用单因素完全随机设计,在肉鸡前期(1~21日龄)和后期(22~42日龄)基础饲粮中分别添加0、100、200、400和800 mg/kg外源蛋白酶,通过单胃动物仿生消化系统(SDS-Ⅲ)建立肉鸡体外模拟消化模型,测定体外降解效率。每个处理5个重复,每个重复1根仿生消化管。结果表明:肉鸡前期饲粮中添加蛋白酶能显著提高饲粮总能消化率(GED)和酶水解物能值(EHGE)(P<0.05),200 mg/kg蛋白酶组的干物质消化率(DMD)显著高于对照组(P<0.05),且肉鸡前期饲粮DMD、GED和EHGE与外源蛋白酶添加量呈显著先升高后降低的二次线性关系(P<0.05);肉鸡后期饲粮中添加蛋白酶能显著提高饲粮DMD、GED和EHGE(P<0.05),且三者与外源蛋白酶添加量呈显著先升高后降低的二次线性关系(P<0.05)。由此可见,玉米-豆粕型基础饲粮中添加适量外源蛋白酶,可显著提高肉鸡饲粮体外降解效率;在本试验中,200 mg/kg蛋白酶组体外降解效率最高。

本文引用格式

王雄, 唐小懿, 陈凤鸣, 陈清华 . 外源蛋白酶对肉鸡饲粮体外降解效率的影响[J]. 动物营养学报, 2018 , 30(8) : 3184 -3188 . DOI: 10.3969/j.issn.1006-267x.2018.08.037

Abstract

The experiment was conducted to investigate the effects of exogenous protease on in vitro degradation efficiency of broiler diets and obtain the optimum protease supplementation in basal diets for broilers, and aiming to provide a theoretical reference for rapidly evaluating the effectiveness of protease. A single factor completely randomized design was adopted to add exogenous protease with 0, 100, 200, 400 and 800 mg/kg in basal diets for starter (1 to 21 days of age) and finisher (22 to 42 days of age) phases of broilers, respectively, and an in vitro simulated digestion model for broilers by using a simulated digestion system for monogastric animals (SDS-Ⅲ) was established to determine in vitro degradation efficiency. Each treatment contained 5 replicates with 1 digestion tube per replicate. The results showed that adding protease significantly increased the gross energy digestibility (GED) and enzymatic hydrolysate gross energy (EHGE) of basal diets in starter phases (P<0.05), and the dry matter digestibility (DMD) in 200 mg/kg protease group was significantly higher than that in the control group (P<0.05). Protease significantly increased DMD, GED and EHGE of basal diets in finisher phases (P<0.05). Moreover, there was a quadratic linear relationship between the levels of DMD, GED and EHGE and the amount of exogenous protease, and the DMD, GED and EHGE increased first and then decreased in diets in both starter and finisher phases (P<0.05). Thus, the corn-soybean meal basal diet supplemented with appropriate amount of exogenous protease can significantly improve in vitro degradation efficiency of broiler diets. In this experiment, in vitro degradation efficiency in 200 mg/kg protease group is the highest.

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