为探讨鸡血藤醇提物对黄颡鱼抗氧化能力、非特异性免疫力和抗病力的影响，选取健康1龄全雄黄颡鱼720尾，初始体重为（35.25±1.63）g，初始体长为（14.07±0.24）cm，随机分为6组（每组3个重复，每个重复40尾），分别投喂添加0、0.04%、0.08%、0.16%、0.32%和0.64%鸡血藤醇提物的等氮等能饲料，依次标记为T1~T6组，并以T1组为对照组。饲养60 d后测定黄颡鱼各组织（鳃、脑、头肾、中肾、肝胰脏、脾脏）及血清中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）活性与丙二醛（MDA）、谷胱甘肽（GSH）、一氧化氮（NO）含量，以及肝胰脏及血清中谷丙转氨酶（GPT）、谷草转氨酶（GOT）活性与血清中补体3（C3）、免疫球蛋白（IgM）、白蛋白（ALB）含量。饲养试验结束后，通过注射嗜水气单胞菌进行攻毒，计算攻毒7 d后的累积死亡率和免疫保护率。结果显示：添加0.16%~0.32%鸡血藤醇提物能显著提高黄颡鱼各组织及血清中SOD、CAT、GSH-Px活性与GSH含量以及血清中C3和ALB含量（P<0.05），并能显著降低黄颡鱼各组织及血清中MDA含量（P<0.05）；GOT和GPT活性在肝胰脏中随着鸡血藤醇提物添加量的增加呈先上升后下降的趋势，但二者在血清中呈相反的趋势；各组血清中IgM含量没有显著差异（P>0.05）；肝胰脏、血清、头肾中NO含量随着鸡血藤醇提物添加量的增加呈先上升后下降的趋势，鳃、脑中NO含量则呈先下降后上升的趋势，脾脏和中肾中NO含量不随鸡血藤醇提物添加量的变化而显著变化（P>0.05）；攻毒试验中，饲料中添加0.16%~0.32%鸡血藤醇提物能显著降低攻毒7 d后的累积死亡率（P<0.05），并有效提高免疫保护率。由此得出，综合抗氧化能力、非特异性免疫力和抗病力，黄颡鱼饲料中鸡血藤醇提物的适宜添加量为0.16%~0.32%。

The present study aimed to evaluate the effects of Caulis spatholobi ethanol extract on the antioxidant ability, non-specific immunity and disease resistance of Pelteobagrus fulvidraco. Seven hundred and twenty healthy Pelteobagrus fulvidraco with the initial body weight of (35.25±1.63) g and initial body length of (14.07±0.24) cm, were selected, and then randomly divided into 6 groups with three replicates per group and 40 fish per replicate. Fish in the 6 groups were fed isonitrogenous and isoenergic diets supplemented with 0, 0.04%, 0.08%, 0.16%, 0.32% and 0.64% Caulis spatholobi ethanol extract, and marked as T1 to T6 groups in order. T1 group was set as the control group. After a 60-day feeding trial, the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px), the contents of malondialdehyde (MDA), glutathione (GSH) and nitric oxide (NO) in serum and various tissues (gill, brain, head kidney, middle kidney hepatopancreas and spleen), the activities of glutamic-oxaloacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT) in hepatopancreas and serum, and the contents of complement 3 (C3), albumin (ALB) and immune globulin M (IgM) in serum were analyzed. After the feeding trial, the cumulative mortality and immune protective rate were calculated during 7 days after the challenge with injected Aeromonas hydrophila. The results showed that adding 0.16% to 0.32% Caulis spatholobi ethanol extract significantly improved the activities of SOD, CAT, GSH-Px and the content of GSH in different tissues and serum (P<0.05), significantly increased the contents of C3 and ALB in serum (P<0.05), and significantly reduced the content of MDA in different tissues and serum of Pelteobagrus fulvidraco (P<0.05). The activities of GOT and GPT in hepatopancreas were increased firstly and then decreased with the addition of Caulis spatholobi alcohol extract increasing, but showed an opposite trend in serum. However, the IgM content in serum had no significant difference among groups (P>0.05). The content of NO in hepatopancreas, serum and head kidney was raised firstly and then decreased with the addition of Caulis spatholobi alcohol extract increasing. In contrast, with the addition of Caulis spatholobi alcohol extract increasing, the content of NO was decreased firstly and then raised, and it in spleen and middle kidney had no significant change (P<0.05). In challenge test, adding 0.16% to 0.32% Caulis spatholobi ethanol extract significantly reduced the cumulative mortality rate (P<0.05), and effectively improved the immune protective rate. In summary, comprehensive antioxidant ability, non-specific immunity and disease resistance, the appropriate addition of Caulis spatholobi ethanol extract in diets for Pelteobagrus fulvidraco is 0.16% to 0.32%.