饲料营养 Feed science and technology

高脂饲料中添加绿原酸对草鱼生长性能和脂质代谢的影响

展开
  • 1. 江西省农业科学院, 南昌 330200;
    2. 四川农业大学动物科技学院, 成都 611130
杨天俊(1989-),男,四川泸州人,硕士,研究方向为特种经济动物养殖。E-mail:584846918@qq.com

收稿日期: 2018-01-26

  网络出版日期: 2018-08-18

基金资助

国家自然科学基金地区科学基金项目(31660743);江西省特种水产产业技术体系(JXARS-10);江西现代农业科研协同创新专项(JXXTCX2015004-009,JXXTC201602-03);江西省青年科学基金项目(20171BAB214015)

Effects of Chlorogenic Acid Supplementation in High-Fat Diets on Growth Performance and Lipid Metabolism of Grass Carp(Ctenopharyngodon idellus)

Expand
  • 1. Jiangxi Academy of Agricultural Science, Nanchang 330200, China;
    2. College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2018-01-26

  Online published: 2018-08-18

摘要

本试验旨在探讨高脂饲料中添加绿原酸(CGA)对草鱼生长性能和脂质代谢的影响。选择健康、初始体重为(7.53±0.30)g的草鱼360尾,随机分为4组,C(对照)、200CGA、400CGA、600CGA组分别饲喂CGA添加水平为0、200、400、600 mg/kg的高脂饲料(粗脂肪含量约为9.00%),每组3重复,每重复30尾,饲喂11周。结果表明,与C组相比:1)400CGA组鱼终末体重、增重率、蛋白质效率均显著提高(P<0.05),其中终末体重增加15.90%,增重率提高22.96%,蛋白质效率提升17.75%,同时饲料系数显著降低(P<0.05),降低14.86%;2)200CGA、400CGA组血清总胆固醇(T-CHO)、低密度脂蛋白胆固醇(LDL-C)含量显著降低(P<0.05),试验组血清高密度脂蛋白胆固醇(HDL-C)含量显著提高(P<0.05);3)试验组肝胰脏甘油三酯(TG)、T-CHO、LDL-C含量显著降低(P<0.05),肝胰脏肝脂酶(HL)、总脂酶(TL)活力显著提高(P<0.05),400CGA、600CGA组肝胰脏HDL-C含量显著提高(P<0.05),400CGA组肝胰脏脂蛋白脂酶(LPL)活力显著提高(P<0.05);4)透射电镜切片结果显示,400CGA、600CGA组肝胰脏细胞内脂滴体积小、数量少、分布较稀疏。结果提示,高脂饲料中CGA添加水平为400 mg/kg时,可促进草鱼机体脂质代谢,改善生长性能。

本文引用格式

杨天俊, 陈彦良, 刘文舒, 郭小泽, 唐艳强, 刘玉廷, 黎徳兵, 李思明 . 高脂饲料中添加绿原酸对草鱼生长性能和脂质代谢的影响[J]. 动物营养学报, 2018 , 30(8) : 3219 -3228 . DOI: 10.3969/j.issn.1006-267x.2018.08.041

Abstract

This study aimed to investigate the effects of chlorogenic acid (CGA) supplementation in high-fat diets on growth performance, lipid metabolism of grass carp(Ctenopharyngodon idellus). A total of 360 healthy and grass carp with an initial body weight (7.53±0.30) g were randomly divided into 4 groups with 3 replicates per group and 30 craps per replicate. Craps in C (control), 200CGA, 400CGA and 600CGA groups were fed high-fat diet (crude lipid was about 9.00%) supplemented with 0, 200, 400 and 600 mg/kg CGA for 11 weeks, respectively. The results showed that compared with C group:1) 400CGA group had significantly higher final body weight (FBW), weight gain rate (WGR) and protein efficiency ratio (PER) of diet (P<0.05), FBW increased by 15.90%, WGR increased by 22.96%m and PER increased by 17.75%, 400CGA group had significantly lower feed coefficient (P<0.05), which was decreased by 14.86%. 2) 200CGA and 400CGA groups had significantly lower serum contents of total cholesterol (T-CHO) and low density lipoprotein cholesterol (LDL-C) (P<0.05), experimental groups had significantly higher serum content of high density lipoprotein cholesterol (HDL-C) (P<0.05). 3) Experimental groups had significantly lower contents of triglyceride (TG), T-CHO and LDL-C in hepatopancreas (P<0.05), and significantly higher activities of lipase(HL) and total lipase(TL) in hepatopancreas (P<0.05); 400CGA and 600CGA groups had significantly higher HDL-C content in hepatopancreas (P<0.05); 400CGA group had significantly higher lipoprotein lipase (LPL) activity in hepatopancreas (P<0.05). 4) Hepatopancreas sections under transmission electron microscope showed that lipid droplets were smaller, littleness and scattered in 400CGA and 600CGA groups. As a result, the supplementation of 400 mg/kg CGA in high-fat diets can promote lipid metabolism and growth performance of grass carp.

参考文献

[1] 尾崎久雄.鱼类消化生理[M].吴尚忠,译.上海:上海科学技术出版社,1983.

[2] GRISDALE-HELLAND B,HELLAND S J.Replacement of protein by fat and carbohydrate in diets for atlantic salmon (Salmo salar) at the end of the freshwater stage[J].Aquaculture,1997,152(1/2/3/4):167-180.

[3] CHO C Y,KAUSHIK S J.Nutritional energetics in fish:energy and protein utilization in rainbow trout (Salmo gairdneri)[J].World Review of Nutrition and Dietetics,1990,61:132-172.

[4] DU Z Y,LIU Y J,TIAN L X,et al.Effect of dietary lipid level on growth, feed utilization and body composition by juvenile grass carp(Ctenopharyngodon idella)[J]. Aquaculture Nutrition,2005,11(2):139-146.  

[5] LEE S M,JEON I G,LEE J Y.Effects of digestible protein and lipid levels in practical diets on growth,protein utilization and body composition of juvenile rockfish (Sebastes schlegeli)[J].Aquaculture,2002,211(1/2/3/4):227-239.

[6] REGOST C,ARZEL J,CARDINAL M,et al.Dietary lipid level,hepatic lipogenesis and flesh quality in turbot (Psetta maxima)[J].Aquaculture,2001,193(3/4):291-309.

[7] PERES H,OLIVATELES A.Effect of dietary lipid level on growth performance and feed utilization by European sea bass juveniles (Dicentrarchus labrax)[J].Aquaculture,1999,179(1/2/3/4):325-334.

[8] 王芸,李正,李健,等.绿原酸对凡纳滨对虾抗氧化系统及抗低盐度胁迫的影响[J].生态学报,2013,33(18):5704-5713.

[9] TG G,DMⅢ G.Dietary lipid level but not L-carnitine affects growth performance of hybrid striped bass (Morone chrysops ♀×M.saxatilis)[J].Aquaculture,2000,190(3/4):237-246.

[10] FARAH A,MONTEIRO M,DONANGELO C M,et al.Chlorogenic acids from green coffee extract are highly bioavailable in humans[J].The Journal of Nutrition,2009,138(12):2309-2315.

[11] LI S Y,BIAN H T,LIU Z,et al.Chlorogenic acid protects MSCs against oxidative stress by altering FOXO family genes and activating intrinsic pathway[J].European Journal of Pharmacology,2012,674(2/3):65-72.

[12] LEPELLEY M,CHEMINADE G,TREMILLON N,et al.Chlorogenic acid synthesis in coffee:An analysis of CGA content and real-time RT-PCR expression of HCT, HQT, C3H1,and CCoAOMT1 genes during grain development in C.canephora[J].Plant Science,2007,172(5):978-996.  

[13] RUAN Z,YANH Y H,ZHOU Y,et al.Metabolomic analysis of amino acid and energy metabolism in rats supplemented with chlorogenic acid[J].Amino Acids,2014,46(9):2219-2229.  

[14] BEAM J R,GIBSON A L,KERKSICK C M,et al.Effect of post-exercise caffeine and green coffee bean extract consumption on blood glucose and insulin concentrations[J].Nutrition,2015,31(2):292-297.  

[15] SHI H Y,DONG L,BAI Y H,et al.Chlorogenic acid against carbon tetrachloride-induced liver fibrosis in rats[J].European Journal of Pharmacology,2009,623(1/2/3):119-124.

[16] WANG Y,LI Z,LI J,et al.Effects of dietary chlorogenic acid on growth performance,antioxidant capacity of white shrimp Litopenaeus vannamei under normal condition and combined stress of low-salinity and nitrite[J].Fish & Shellfish Immunology,2015,43(2):337-345.  

[17] SUN L J,SUN J J,THAVARAJ P,et al.Effects of thinned young apple polyphenols on the quality of grass carp (Ctenopharyngodon idellus) surimi during cold storage[J].Food Chemistry,2017,224:372-381.

[18] 张纯.绿原酸对建鲤生长、抗氧化和非特异性免疫功能的影响[D].硕士学位论文.雅安:四川农业大学,2011.

[19] 王芸,李健,李正,等.绿原酸诱导凡纳滨对虾抗氧化功能及抵御低盐度胁迫的响应[J].中国水产科学,2014,21(2):340-350.

[20] 余小领.冷冻和解冻工艺对猪肉保水性和组织结构的影响研究[D].博士学位论文.南京:南京农业大学,2007.

[21] OLTHOF M R,HOLLMAN P C,KATAN M B.Chlorogenic acid and caffeic acid are absorbed in humans[J].The Journal of Nutrition,2001,131(1):66-71.  

[22] LAFAY S,GIL-IZQUIERDO A,MANACH C,et al.Chlorogenic acid is absorbed in its intact form in the stomach of rats[J].The Journal of Nutrition,2006,136(5):1192-1197.  

[23] 肖洋.绿原酸对中华鳖生长性能和非特异性免疫的影响[D].硕士学位论文.雅安:四川农业大学,2010.

[24] 朱卫,刘鉴毅,庄平,等.饲料脂肪水平对点篮子鱼生长和体成分的影响[J].海洋渔业,2013,35(1):65-71.

[25] 李祥英.自拟中草药添加剂对绵羊育肥效果的观察[J].中兽医学杂志,2008(6):51.

[26] 赵国志.油脂在饲料中的应用[J].粮食与饲料工业,2000(8):14-15.

[27] SHIMODA H,SEKI E,AITANI M.Inhibitory effect of green coffee bean extract on fat accumulation and body weight gain in mice[J].BMC Complementary and Alternative Medicine,2006,6(1):9.

[28] DE SOTILLO D V R,HADLEY M.Chlorogenic acid modifies plasma and liver concentrations of:cholesterol,triacylglycerol,and minerals in (fa/fa) Zucker rats[J].The Journal of Nutritional Biochemistry,2002,13(12):717-726.  

[29] FRANK J,KAMAL-ELDIN A,RAZDAN A,et al.The dietary hydroxycinnamate caffeic acid and its conjugate chlorogenic acid increase vitamin e and cholesterol concentrations in Sprague-Dawley rats[J].Journal of Agricultural and Food Chemistry,2008,51(9):2526-2531.

[30] 张兰涛,常翠青,刘阳,等.氯原酸对db/db小鼠糖脂代谢紊乱的影响及其作用机制[J].中国医学科学院学报,2011,33(3):281-286.

[31] 王强,陈东辉,邓文龙.金银花提取物对血脂与血糖的影响[J].中药药理与临床,2007,23(3):40-42.

[32] FAULCONNIER Y,BONNET M,BOCQUIER F,et al.Effects of photoperiod and feeding level on adipose tissue and muscle lipoprotein lipase activity and mRNA level in dry non-pregnant sheep[J].British Journal of Nutrition,2001,85(3):299-306.  

[33] 严瑾,范春雷.人类肝脂肪酶的研究进展[J].中国动脉硬化杂志,2003,11(6):596-600.

[31] 周永梅,陈敬文,赖续文,等.冷冻切片锇酸染色法在脂肪染色中的应用[J].临床与实验病理学杂志,2016,32(4):467-468.

[35] RUYTER B,MOYA-FALCÍN C,ROSENLUND G,et al.Fat content and morphology of liver and intestine of Atlantic salmon (Salmo salar):effects of temperature and dietary soybean oil[J].Aquaculture,2006,252(2/3/4):441-452.
文章导航

/