水产营养 Aquaculture Nutrition

投喂频率对虎斑乌贼生长性能、体成分、消化酶活性、组织脂肪酸和氨基酸含量的影响

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  • 宁波大学海洋学院鱼类营养研究室, 宁波 315211
朱婷婷(1993-),女,浙江温州人,硕士研究生,从事水生动物营养与饲料研究。E-mail:648294230@qq.com

收稿日期: 2018-02-28

  网络出版日期: 2018-09-20

基金资助

宁波市重大专项(201401C1111001)

Effects of Feeding Frequency on Growth Performance, Body Composition, Digestive Enzyme Activities, Tissue Fatty Acid and Amino Acid Compositions of Sepia pharaonis

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  • Laboratory of Fish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China

Received date: 2018-02-28

  Online published: 2018-09-20

摘要

本试验旨在研究投喂频率对虎斑乌贼生长性能、体成分、消化酶活性、组织脂肪酸和氨基酸含量的影响。试验选用初始体重为(13.08±0.01)g的虎斑乌贼幼体180只,随机分成3组(投喂频率分别为每天1、2和4次,分别命名为F1、F2和F4投喂组),每组3个重复,每个重复20只。试验期4周。结果表明:1)F2和F4投喂组的增重率、特定生长率和肝体比均显著高于F1投喂组(P<0.05),F1和F4投喂组的饵料效率显著低于F2投喂组(P<0.05)。2)F4投喂组的肝脏水分含量显著低于F1投喂组(P<0.05),肝脏粗脂肪含量显著高于F1和F2投喂组(P<0.05)。F2和F4投喂组的肌肉水分含量显著低于F1投喂组(P<0.05),肌肉粗蛋白质和粗脂肪含量显著高于F1投喂组(P<0.05)。3)各组肌肉的总氨基酸、必需氨基酸和非必需氨基酸含量没有显著性差异(P>0.05)。F1投喂组的肝脏总氨基酸、必需氨基酸和非必需氨基酸含量显著高于F2和F4投喂组(P<0.05),F2投喂组的肝脏总氨基酸和非必需氨基酸含量与F4投喂组之间没有显著性差异(P>0.05)。4)F1投喂组的肌肉和肝脏的二十二碳六稀酸(DHA)含量和DHA/二十碳五稀酸(EPA)显著高于F2和F4投喂组(P<0.05),而F2和F4投喂组之间没有显著性差异(P>0.05)。各组肌肉和肝脏的多不饱和脂肪酸和n-3高不饱和脂肪酸含量没有显著性差异(P>0.05)。5)F1投喂组的肝脏谷胱甘肽过氧化物酶活性显著高于F2投喂组(P<0.05)。F1投喂组的前肠α-淀粉酶、脂肪酶和碱性磷酸酶活性均显著高于F2投喂组(P<0.05),F2和F4投喂组之间没有显著性差异(P>0.05)。由此可见,虎斑乌贼幼体养殖的适宜投喂频率为每天2次。

本文引用格式

朱婷婷, 李晨晨, 陆游, 金敏, 王学习, 罗嘉翔, 周歧存 . 投喂频率对虎斑乌贼生长性能、体成分、消化酶活性、组织脂肪酸和氨基酸含量的影响[J]. 动物营养学报, 2018 , 30(9) : 3581 -3592 . DOI: 10.3969/j.issn.1006-267x.2018.09.028

Abstract

This experiment was conducted to investigate the effects of feeding frequency on growth performance, body composition, digestive enzyme activities, tissue fatty acid and amino acid compositions of Sepia pharaonis. A total of 180 juvenile Sepia pharaonis with an initial body weight of (13.08±0.01) g were randomly divided into 3 groups (feeding frequencies were 1, 2 and 4 times a day, named F1, F2 and F4 groups, respectively), and each group had 3 replicates of 20 Sepia pharaonis per replicate. The experiment lasted for 4 weeks. The results showed as follows:1) the weight gain rate (WGR), specific growth rate (SGR) and hepatosomatic index (HSI) in F2 and F4 groups were significantly higher than those in F1 group (P<0.05), however, the feed efficiency (FE) in F1 and F4 groups was significantly lower than that in F2 group (P<0.05). 2) The liver moisture content in F4 group was significantly lower than that in F1 group (P<0.05), and the liver crude lipid content was significantly higher than that in F1 and F2 groups (P<0.05). The muscle moisture content in F2 and F4 groups was significantly lower than that in F1 group (P<0.05), and the muscle crude protein and crude lipid contents was significantly higher than that in F1 group (P<0.05). 3) There were no significant differences in the contents of total amino acids (TAA), essential amino acids (EAA) and non-essential amino acids (NEAA) in muscle among all groups (P>0.05). The contents of TAA, EAA and NEAA in liver in F1 group were significantly lower than those in F2 and F4 groups (P<0.05), the contents of TAA and NEAA in liver in F1 group had no significant difference compared with F4 group (P>0.05). 4) The docosahexaenoic acid (DHA) content and DHA/eicosapentaenoic acid (EPA) in muscle and liver in F1 group were significantly higher than those in F2 and F4 groups (P<0.05), and there were no significant differences in DHA content and DHA/EPA in muscle and liver between F2 and F4 groups (P>0.05). There were no significant differences in the contents of polyunsaturated fatty acids (PUFA) and n-3 high unsaturated fatty acids (n-3HUFA) in muscle and liver among all groups (P>0.05). 5) The liver glutathione peroxidase activity in F1 group was significantly higher than that in F2 group (P<0.05). The activities of α-amylase, lipase and alkaline phosphatase in anterior intestine in F1 group were significantly higher than those in F2 group (P<0.05), but no significant differences were found between F2 and F4 groups (P>0.05). According to the experimental results, the appropriate feeding frequency for juvenile Sepia pharaonis is 2 times a day.

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