动物营养学报 >

2018 , Vol. 30 >Issue 9: 3726 - 3733

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.09.044

饲料安全 Feed Security

脱氧雪腐镰刀菌烯醇暴露对仔猪海马神经细胞神经递质、脂质过氧化及钙稳态的影响

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  • 安徽农业大学动物科技学院, 合肥 230036
王希春(1978-),男,安徽利辛人,副教授,博士,从事畜禽营养代谢病与中毒病研究。E-mail:wangxichun@ahau.edu.cn

收稿日期: 2018-03-13

  网络出版日期: 2018-09-20

基金资助

国家自然科学基金项目（31472250）；安徽省生猪产业技术体系资助项目（AHCYJSTX-05）

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Effects of Deoxynivalenol Exposure on Neurotransmitter, Lipid Peroxidation and Calcium Homeostasis in Piglet Hippocampal Nerve Cells

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  • College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China

Received date: 2018-03-13

  Online published: 2018-09-20

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摘要

本试验旨在研究脱氧雪腐镰刀菌烯醇（DON）暴露对体外培养的仔猪海马神经细胞中神经递质、脂质过氧化及钙稳态的影响，探讨DON的神经毒性作用。以不同质量浓度的DON（0、125、250、500、1 000和2 000 ng/mL）处理仔猪海马神经细胞24 h后，检测细胞中5-羟色胺（5-HT）、多巴胺（DA）、乙酰胆碱（ACH）、γ-氨基丁酸（GABA）、去甲肾上腺素（NE）含量，总抗氧化能力（T-AOC）、谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性和一氧化氮（NO）、丙二醛（MDA）含量，钙离子（Ca2+）、钙调蛋白（CaM）含量以及CaM mRNA相对表达量。结果表明：与对照组相比，1）当DON浓度为1 000 ng/mL时，5-HT、DA、ACH含量显著升高（P<0.05），GABA含量极显著升高（P<0.01），而NE含量显著降低（P<0.05）；当DON浓度为2 000 ng/mL时，5-HT、DA、ACH含量极显著升高（P<0.01）。2）当DON浓度为250 ng/mL时，SOD活性和T-AOC极显著降低（P<0.01）；当DON浓度为1 000 ng/mL时，GSH-Px活性显著降低（P<0.05），NO含量极显著降低（P<0.01），而MDA含量极显著升高（P<0.01）；当DON浓度为2 000 ng/mL时，CAT活性显著降低（P<0.05），GSH-Px活性极显著降低（P<0.01）。3）当DON浓度为125 ng/mL时，CaM含量极显著升高（P<0.01）；当DON浓度为250 ng/mL及以上时，Ca2+含量和CaM mRNA相对表达量极显著升高（P<0.01）。综上，DON暴露可改变仔猪海马神经细胞神经递质的分泌及脂质过氧化，并影响钙稳态，对仔猪海马神经细胞具有一定的神经毒性作用。

关键词： 脱氧雪腐镰刀菌烯醇; 仔猪海马神经细胞; 神经递质; 脂质过氧化; 钙稳态

本文引用格式

王希春, 储小燕, 张娅菲, 朱雷, 范梦雪, 冯士彬, 李玉, 吴金节 . 脱氧雪腐镰刀菌烯醇暴露对仔猪海马神经细胞神经递质、脂质过氧化及钙稳态的影响[J]. 动物营养学报, 2018 , 30(9) : 3726 -3733 . DOI: 10.3969/j.issn.1006-267x.2018.09.044

Abstract

This experiment was conducted to investigate the effects of deoxynivalenol (DON) exposure on neurotransmitters, lipid peroxidation and calcium homeostasis in piglet hippocampal nerve cells (PHNCs) cultured in vitro, to discuss the neurotoxic effects of DON. PHNCs were exposed to DON at different mass concentrations (0, 125, 250, 500, 1 000 and 2 000 ng/mL) for 24 h, and the contents of 5-hydroxytryptamine (5-HT), dopamine (DA), acetylcholine (ACH), γ-aminobutyric acid (GABA) and noradrenaline (NE), total antioxidant capacity (T-AOC), the activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT), the contents of nitric oxide (NO) and malondialdehyde (MDA), the contents of Ca2+ and calmodulin (CaM) and the relative expression of CaM mRNA in cells were determined. The results showed as follows:compared with control group, 1) when the concentration of DON was 1 000 ng/mL, the contents of 5-HT, DA and ACH were significantly increased (P<0.05), the content of GABA was significantly increased (P<0.01), while the content of NE was significantly decreased (P<0.05). When the concentration of DON was 2 000 ng/mL, the contents of 5-HT, DA and ACH were significantly increased (P<0.01). 2) When the concentration of DON was 250 ng/mL, the activities of SOD and T-AOC were significantly decreased (P<0.01). When the concentration of DON was 1 000 ng/mL, the activity of GSH-Px was significantly decreased (P<0.05), and the content of NO was significantly decreased (P<0.01), while the content of MDA was significantly increased (P<0.01). When the concentration of DON was 2 000 ng/mL, the activity of CAT was significantly decreased (P<0.05), and the activity of GSH-Px was significantly decreased (P<0.01). 3) When the concentration of DON was 250 ng/mL, the content of CaM was significantly increased (P<0.01). When the concentration of DON was 250 ng/mL and above, the content of Ca2+ and the relative expression level of CaM mRNA were significantly increased (P<0.01). In conclusion, DON exposure can change the secretions of neurotransmitters, the lipid peroxidation and calcium homeostasis in PHNCs, it suggests that DON has some neurotoxic effects to PHNCs.

Key words： deoxynivalenol; piglet hippocampal nerve cells; neurotransmitter; lipid peroxidation; calcium homeostasis

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