动物营养学报 >

2018 , Vol. 30 >Issue 9: 3759 - 3771

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.09.048

研究简报 Short communications

不同饲料添加剂对杜寒杂交肉羊体脂脂肪酸组成和氧化稳定性的影响

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  • 内蒙古农业大学动物科学学院, 呼和浩特 010018
刘旺景(1991-),男,山西孝义人,博士研究生,研究方向为动物营养与饲料。E-mail:wangjingliu2016@foxmail.com

收稿日期: 2018-01-29

  网络出版日期: 2018-09-20

基金资助

内蒙古自治区重大专项"中国-加拿大肉羊养殖及饲草料品种开发与利用".

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Effects of Different Feed Additives on Fatty Acid Composition and Oxidation Stability of Body Fat in Dorper×Thin-Tailed Han Crossbred Mutton Lambs

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  • College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2018-01-29

  Online published: 2018-09-20

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摘要

本试验旨在研究不同饲料添加剂对杜寒杂交肉羊体脂脂肪酸组成和氧化稳定性的影响。选取30只健康、体重[（42.5±3.1）kg]相近的6月龄杜寒杂交F1代肉羊，采用单因素完全随机区组试验设计，共分为3组，每组10只。对照组（G1组）试验羊饲喂基础饲粮，试验1组（G2组）试验羊饲喂添加了沙葱粉的基础饲粮，沙葱粉按照每只每天20 g的量添加，试验组（G3组）试验羊饲喂添加了微生物发酵饲料的基础饲粮，微生物发酵饲料按照每只每天100 g的量添加。试验期为75 d，其中预试期为15 d，正试期为60 d。在饲养试验结束后，每组随机选取3只羊进行屠宰，分别从肾脏周围、腹部皮下以及尾部采集脂肪样品，测定其脂肪酸组成以及丙二醛（MDA）含量和超氧化物歧化酶（SOD）活性。结果显示：与G1组相比，1）G2组和G3组肉羊平均日增重显著提高（P<0.05），料重比显著降低（P<0.05）；2）G2组肾周、腹部皮下以及尾部脂肪组织中亚油酸（C18：2cis-6）、α-亚麻酸（C18：3n-3）、二十碳五烯酸（EPA，C20：5n-3）、二十二碳六烯酸（DHA，C22：6n-3）、多不饱和脂肪酸（PUFA）、n-6 PUFA的含量以及PUFA/饱和脂肪酸（SFA）的值显著提高（P<0.05），硬脂酸（C18：0）的含量显著降低（P<0.05），G3组腹部皮下和尾部脂肪组织中DHA的含量显著提高（P<0.05），肾周脂肪组织中单不饱和脂肪酸（MUFA）和C18：0的含量显著降低（P<0.05）；3）G2组和G3组尾部脂肪组织中n-6 PUFA的含量以及n-6/n-3的值显著提高（P<0.05），G2组肾周脂肪组织中n-3 PUFA的含量显著提高（P<0.05），G3组肾周脂肪组织中PUFA/SFA的值显著降低（P<0.05）；4）G2组和G3组腹部皮下和肾周脂肪组织中SOD的活性显著提高（P<0.05），MDA的含量显著降低（P<0.05）；5）肉羊腹部皮下（R2=0.967）、尾部（R2=0.965）和肾周脂肪组织（R2=0.992）中MDA含量和SOD活性与PUFA沉积量之间存在线性关系（P<0.001），其中MDA对多元回归方程的贡献为负增加，而SOD为正增加。综上所述，饲粮中添加沙葱粉和微生物发酵饲料均能够提高舍饲杜寒杂交肉羊的生长性能；饲粮中添加沙葱粉可有效改善杜寒杂交肉羊体脂脂肪酸组成，并可提高脂肪氧化稳定性，改善脂肪品质；饲粮中添加微生物发酵饲料对杜寒杂交肉羊体脂脂肪酸组成改善效果欠佳，但可提高脂肪的氧化稳定性。

关键词： 饲料添加剂; 肉羊; 脂肪品质; 抗氧化物; 氧化稳定性; 多不饱和脂肪酸

本文引用格式

刘旺景, 敖长金, 丁赫, 哈斯额尔敦, 丹妮, 李书仪, 李英, 张佐忠 . 不同饲料添加剂对杜寒杂交肉羊体脂脂肪酸组成和氧化稳定性的影响[J]. 动物营养学报, 2018 , 30(9) : 3759 -3771 . DOI: 10.3969/j.issn.1006-267x.2018.09.048

Abstract

The objective of this experiment was to investigate the effects of different feed additives on fatty acid composition and oxidation stability of body fat in Dorper×thin-tailed Han crossbred mutton lambs. Single factor completely randomized block design was used in the current study. A total of 30 healthy and six-month-old Dorper×thin-tailed Han crossbred F1 mutton lambs with the similar body weight[(42.5±3.1) kg] were randomly divided into 3 groups with 10 lambs each. Lambs in control group (G1 group) were fed a basal diet, those in experimental group 1 (G2 group) were fed the basal diet supplemented with Allium mongolicum Regel powder (20 g/d each lamb), and those in experimental group 2 (G3 group) were fed the basal diet supplemented with microbial fermented feed (100 g/d each lamb). The trial period was 75 d, which consisted of 15 d of per-feeding period and 60 d of formal period. At the end of feeding experiment, three lambs were randomly selected from each group and slaughtered to collect the perirenal adipose tissue, abdominal subcutaneous adipose tissue and tail adipose tissue to measure fatty acid composition, malondialdehyde (MDA) content and superoxide dismutase (SOD) activity. The results showed as follows:compared with G1 group, 1) G2 group and G3 group showed significantly higher average daily gain and significantly lower feed to gain ratio of mutton lambs (P<0.05). 2) G2 group showed significantly higher the contents of linoleic acid (C18:2cis-6), α-linolenic acid (C18:3n-3), eicosapentaenoic acid (EPA, C20:5n-3), docosahexaenoic acid (DHA, C22:6n-3), polyunsaturated fatty acids (PUFA), n-6 PUFA and the value of PUFA/saturated fatty acid (SFA) (P<0.05) and significantly lower the content of C18:0 in the perirenal adipose tissue, abdominal subcutaneous adipose tissue and tail adipose tissue (P<0.05); G3 group showed significantly higher DHA content in the abdominal subcutaneous adipose tissue and tail adipose tissue (P<0.05), and showed significantly lower the contents of monounsaturated fatty acids (MUFA) and C18:0 in the perirenal adipose tissue (P<0.05). 3) The contents of n-6 PUFA and the value of n-6/n-3 in the tail adipose tissue in G2 group and G3 group were significantly increased (P<0.05), the content of n-3 PUFA in the perirenal adipose tissue in G2 group was significantly increased (P<0.05), and the value of PUFA/SFA in the perirenal adipose tissue in G3 group was significantly decreased (P<0.05). 4) G2 group and G3 group significantly up-regulated the SOD activity and significantly down-regulated the MDA content in the perirenal adipose tissue and abdominal subcutaneous adipose tissue (P<0.05). 5) There were linear relationships between the MDA content, SOD activity and PUFA deposition (P<0.001) in the perirenal adipose tissue (R2=0.992), abdominal subcutaneous adipose tissue (R2=0.967) and tail adipose tissue (R2=0.965). The contribution of MDA was negative for the multiple regression equation, whereas the SOD was positive. In conclusion, diet supplemented with Allium mongilicum Regel powder and microbial fermented feed both can improve the growth performance of Dorper×thin-tailed Han crossbred mutton lambs. Diet supplemented with Allium mongilicum Regel powder can improve the fatty acid composition and fat oxidative stability effectively, which is significant in improving the fat quality of Dorper×thin-tailed Han crossbred mutton lambs. Diet supplemented with microbial fermented feed cannot improve the fatty acid composition effectively, but can increase the fat oxidative stability of Dorper×thin-tailed Han crossbred mutton lambs.

Key words： feed additives; mutton lamb; fat quality; antioxidant; oxidation stability; polyunsaturated fatty acids

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