研究简报 Short communications

糖皮质激素和饲粮脂肪水平对肉鸡脂肪代谢的影响

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  • 山东农业大学动物科技学院, 山东省动物生物工程与疾病防治重点实验室, 泰安 271018
王明会(1993-),女,山东聊城人,硕士研究生,研究方向为动物营养与饲料科学。E-mail:wangminghui825@163.com

收稿日期: 2018-02-12

  网络出版日期: 2018-09-20

基金资助

国家自然科学基金项目(31672441,31301993);山东省"双一流"奖补资金;泰山学者项目(201511023);山东农业大学动物科技学院一流学科建设大学生研究训练(SRT)计划(2017028)

Effects of Glucocorticoid and Dietary Fat Level on Lipid Metabolism of Broilers

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  • Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science, Shandong Agricultural University, Tai'an 271018, China

Received date: 2018-02-12

  Online published: 2018-09-20

摘要

本试验旨在研究糖皮质激素——地塞米松(DEX)和饲粮脂肪水平对肉鸡脂肪代谢的影响。选取22日龄体重相近的爱拔益加(AA)肉鸡200只,随机分成低脂饲粮(LFD)和高脂饲粮(HFD)2个处理,每个处理100只,饲喂至38日龄。在35日龄时每个饲粮处理下设2个子处理,分别在皮下注射DEX(2.0 mg/kg BW)和等体积的生理盐水,每个子处理50只,连续注射3 d。结果表明:1)LFD和HFD饲喂条件下,糖皮质激素均显著降低肉鸡体重和腿肌重(P<0.05),显著提高肝脏比重和血液甘油三酯(TG)含量(P<0.05)。2)LFD饲喂条件下,糖皮质激素显著提高腹脂比重及胸肌、腿肌的TG含量(P<0.05)。3)HFD饲喂条件下,糖皮质激素显著提高腹脂和腿肌的肉碱脂酰转移酶1(CPT1)含量(P<0.05),显著降低肝脏和胸肌的CPT1含量(P<0.05)。4)糖皮质激素导致糖皮质激素受体(GR)、过氧化物酶体增殖物激活受体α(PPARα)、脂肪酸转运蛋白1(FATP1)mRNA表达量在腹脂和胸肌中呈整体上调趋势,而在肝脏和腿肌中呈整体下降趋势。结果提示,糖皮质激素可能通过GR、PPARα和FATP1调控肉鸡的脂肪代谢,促进脂肪在肌肉和肝脏的异位沉积。HFD可能通过激活腹脂和腿肌的CPT1,促进脂肪酸的氧化利用,从而缓解糖皮质激素导致的脂肪沉积现象。

本文引用格式

王明会, 刘志梅, 王昊棋, 官家家, 胡庆美, 王晓鹃 . 糖皮质激素和饲粮脂肪水平对肉鸡脂肪代谢的影响[J]. 动物营养学报, 2018 , 30(9) : 3772 -3780 . DOI: 10.3969/j.issn.1006-267x.2018.09.049

Abstract

This study aimed to research the effects of glucocorticoid[dexamethasone (DEX)] and dietary fat level on lipid metabolism of broilers. A total of 200 Arbor Acre broilers with similar body weight were exposed to diets with high (HFD) or low fat level (LFD) from 22 days of age, each treatment had 100 broilers. The diets were fed from 22 to 38 days of age. At 35 to 38 days of age, broilers in each dietary treatment were divided into two sub-treatments, and broilers in which were subcutaneous injected DEX (2 mg/kg BW) and saline with equal volume. Each sub-treatment had 50 broilers. The results showed as follows:1) under the conditions of LFD and HFD, glucocorticoid both significantly decreased body weight and thigh muscle weight (P<0.05), and significantly raised liver proportion and blood concentration of triglyceride (TG) (P<0.05). 2) Under the condition of LFD, glucocorticoid significantly increased abdominal fat proportion and TG content in muscle (P<0.05). 3) Under the condition of HFD, glucocorticoid significantly increased carnitine palmitoyl transferase 1 (CPT1) content in abdominal fat and thigh muscle (P<0.05), while significantly decreased CPT1 content in liver and breast muscle (P<0.05). 4) There were overall up-regulated trends of expression levels of glucocorticoids receptor (GR), peroxisome proliferator-activated receptor α (PPARα) and fatty acid transport protein (FATP1) mRNAs in breast and abdominal fat after the treatment of glucocorticoid, while they were contrary for thigh muscle and liver. The results indicate that glucocorticoid may affect lipid metabolism through regulating GR, PPARα and FATP1, and facilitate the fat ectopic deposit in liver and muscle. High fat diet may activate CPT1 and lipid oxidation in abdominal fat and thigh muscle, thereby alleviate glucocorticoids' effect on fat deposition.

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