动物营养学报 >

2018 , Vol. 30 >Issue 10: 3941 - 3949

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.10.018

反刍与草食动物营养 Ruminant and herbivore nutrition

补喂硫酸钠、微量元素及其组合对哈萨克孕马3种雌激素硫酸盐日排出量的影响

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  • 新疆农业大学, 新疆肉乳用草食动物营养实验室, 乌鲁木齐 830052
李晓斌(1988-),男,甘肃天水人,博士,研究方向为草食动物营养代谢。E-mail:172387243@qq.com

收稿日期: 2018-03-23

  网络出版日期: 2018-10-20

基金资助

十二五农村领域国家科技计划课题（2012BAD45B01）

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Effects of Supplemental Feeding Sodium Sulfate, Trace Elements and Their Combination on Three Kinds of Estrogen Sulfate Daily Outputs of Kazak Pregnancy Horses

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  • Xinjiang Key Laboratory of Meat & Milk Production Herbivore Nutrition, Xinjiang Agricultural University, Urumqi 830052, China

Received date: 2018-03-23

  Online published: 2018-10-20

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摘要

本试验旨在研究补喂硫酸钠、微量元素及其组合对妊娠中后期哈萨克母马血浆和尿中雌酮硫酸钠、马烯雌酮硫酸钠、17α-双氢马烯雌酮硫酸钠含量以及上述3种雌激素硫酸盐日排出量的影响。选择年龄5~7岁、胎次2~4胎的妊娠中期哈萨克母马34匹，随机分为4组，其中对照组9匹、试验Ⅰ组9匹、试验Ⅱ组8匹、试验Ⅲ组8匹，在相同的饲养条件下进行90 d的补饲试验。在自由采食粗饲料的基础上，对照组第1~30天每匹马每天补喂50 g玉米粉，第31~90天补喂100 g玉米粉；试验Ⅰ组第1~30天每匹马每天补喂50 g玉米粉+6 g硫酸钠，第31~90天补喂100 g玉米粉+12 g硫酸钠；试验Ⅱ组第1~30天每匹马每天补喂50 g玉米粉+5 g微量元素，第31~90天补喂100 g玉米粉+5 g微量元素；试验Ⅲ组第1~30天每匹马每天补喂50 g玉米粉+6 g硫酸钠+5 g微量元素，第31~90天补喂100 g玉米粉+12 g硫酸钠+5 g微量元素。结果显示：1）与对照组相比，补喂硫酸钠、微量元素及其组合能够提高孕马血浆中雌酮硫酸钠、马烯雌酮硫酸钠、17α-双氢马烯雌酮硫酸钠的含量，但各组间差异不显著（P>0.05）。2）在试验期内的各采样时间点，各试验组孕马尿中雌酮硫酸钠、马烯雌酮硫酸钠、17α-双氢马烯雌酮硫酸钠的含量与对照组均无显著差异（P>0.05），但各试验组孕马尿中雌酮硫酸钠、马烯雌酮硫酸钠、17α-双氢马烯雌酮硫酸钠的含量在数值上均高于对照组。试验Ⅲ组第30天时孕马尿中上述3种雌激素硫酸盐的含量均最高。3）试验第30天、第60天、第90天，试验Ⅲ组3种雌激素硫酸盐的日排出量均显著或极显著高于对照组（P<0.05或P<0.01）。由此可见，给哈萨克孕马补喂硫酸钠和微量元素的组合能够提高尿中雌酮硫酸钠、马烯雌酮硫酸钠、17α-双氢马烯雌酮硫酸钠的含量，从而显著或极显著提高雌酮硫酸钠、马烯雌酮硫酸钠、17α-双氢马烯雌酮硫酸钠的日排出量。

关键词： 哈萨克孕马; 硫酸钠; 微量元素; 雌激素硫酸盐

本文引用格式

李晓斌, 陈晖, 赵芳, 谢景龙, 杨菊青, 杨开伦 . 补喂硫酸钠、微量元素及其组合对哈萨克孕马3种雌激素硫酸盐日排出量的影响[J]. 动物营养学报, 2018 , 30(10) : 3941 -3949 . DOI: 10.3969/j.issn.1006-267x.2018.10.018

Abstract

The objective of this study was to investigate the effects of supplemental feeding sodium sulfate, trace elements and their combination on plasma and urine sodium estrone sulfate, sodium equilin sulfate, sodium 17α-dihydroequilin sulfate contents and above three kinds of estrogen sulfate daily outputs of Kazak female horses in mid-late pregnancy. Thirty-four 5-to 7-year-old and 2 to 4 fetuses Kazak horses in the middle of pregnancy were divided into 4 groups. The control group had 9 horses, trial group Ⅰ had 9 horses, trial group Ⅱ had 8 horses and trial group Ⅲ had 8 horses. Under the same feeding condition, the supplemental feeding experiment lasted for 90 days. On the 1st to 30th day, horses in the control group, trial group Ⅰ, trial group Ⅱ and trial group Ⅲ were fed 50 g corn flour, 50 g corn flour+6 g sodium sulfate, 50 g corn flour+5 g trace elements, and 50 g corn flour+6 g sodium sulfate+5 g trace elements per horse per day, respectively. On the 31st to 90th day, horses in the control group, trial group Ⅰ, trial group Ⅱ and trial group Ⅲ were fed 100 g corn flour, 100 g corn flour+12 g sodium sulfate, 100 g corn flour+5 g trace elements, and 100 g corn flour+12 g sodium sulfate+5 g trace elements per horse per day, respectively. The results showed as follows:1) compared with the control group, the sodium estrone sulfate, sodium equilin sulfate and sodium 17α-dihydroequilin sulfate contents in plasma in sodium sulfate, trace elements and their combination groups were increased, but the differences were not significant among groups (P>0.05). 2) On the each sampling time points, the sodium estrone sulfate, sodium equilin sulfate and sodium 17α-dihydroequilin sulfate contents in urine of trial groups had no significant differences compared with the control group (P>0.05), but the values were still higher than those in control group. On day 30, above three kinds of estrogen sulfate contents in urine in trial group Ⅲ were all the highest. 3) The sodium estrone sulfate, sodium equilin sulfate, sodium 17α-dihydroequilinsulfate daily outputs in trial group Ⅲ were significantly or extremely significantly higher than those in control group at day 30, 60 and 90 (P<0.05 or P<0.01). It is concluded that supplemental feeding sodium sulfate, trace elements and their combination can increase the contents of urine sodium estrone sulfate, sodium equilin sulfate and sodium 17α-dihydroequilin sulfate of Kazak pregnancy horses, thus increase the daily outputs of sodium estrone sulfate, sodium equilin sulfate and sodium 17α-dihydroequilin sulfate.

Key words： Kazak pregnancy horse; sodium sulfate; trace elements; estrogen sulfate

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