反刍与草食动物营养 Ruminant and herbivore nutrition

瘤胃保护性色氨酸饲粮中添加亚叶酸钙对绵羊血浆色氨酸、犬尿氨酸和褪黑素含量的影响

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  • 新疆农业大学动物科学学院, 新疆肉乳用草食动物营养重点实验室, 乌鲁木齐 830052
王根(1983-),男,山东菏泽人,硕士研究生,研究方向为草食动物营养代谢。E-mail:570610080@qq.com

收稿日期: 2018-04-07

  网络出版日期: 2018-10-20

基金资助

国家自然科学基金项目(31760681)

Effects of Adding Calcium Folate in Rumen Protected Trytophan Diets on Plasma Tryptophan, Kynurenine and Melatonin Contents in Sheep

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  • Xinjiang Agricultural University, Xinjiang Key Laboratory of Meat & Milk Production Herbivore Nutrition, Urumqi 830052, China

Received date: 2018-04-07

  Online published: 2018-10-20

摘要

本试验在瘤胃保护性色氨酸(RPTrp)饲粮中添加2种剂量的亚叶酸钙(CF),研究其对绵羊血浆色氨酸(Trp)、犬尿氨酸(Kyn)和褪黑素(MT)含量的影响,旨在探究调控反刍动物体内MT合成的方法。试验选取(3.0±0.5)岁、平均体重(64.45±3.48)kg的健康萨福克绵羊15只,随机分为3组,每组5只,分别为对照组、试验Ⅰ组和试验Ⅱ组。每天每只羊精料补充料饲喂量为12 g/kg BW,玉米青贮为1.8 kg,RPTrp为222.2 mg/kg BW,自由采食混合干草,在此基础上,试验Ⅰ组添加50 mg的CF、试验Ⅱ组添加100 mg的CF,进行15 d的饲养试验。结果表明:1)上午饲喂后0~12.0 h期间,各组间血浆Trp、Kyn含量差异均不显著(P>0.05);在上午饲喂后6.0、8.0 h时,试验组血浆Trp含量有降低的趋势(P=0.087 2、P=0.053 1);在上午饲喂后4.5、6.0、8.0、10.0 h时,试验组血浆Kyn含量也有降低的趋势(P=0.094 8、P=0.066 7、P=0.090 9、P=0.054 2)。2)上午饲喂后4.5、8.0 h时,试验组血浆5-羟色胺(5-HT)含量与对照组相比有增加的趋势(P=0.080 7、P=0.054 1),在上午饲喂后10.0 h时,试验组极显著升高(P=0.005 7);上午饲喂后6.0、8.0 h时,试验组MT含量也有增加的趋势(P=0.089 0、P=0.070 4),在上午饲喂后10.0 h时,试验组极显著高于对照组(P=0.000 2)。3)上午饲喂前0 h,与对照组相比,试验Ⅱ组血浆总抗氧化能力、谷胱甘肽过氧化物酶活性均显著提高(P<0.05),丙二醛含量显著降低(P<0.05),试验Ⅰ组血浆总抗氧化能力也显著提高(P<0.05)。因此,在RPTrp(222.2 mg/kg·BW)饲粮中每天每只绵羊添加50或100 mg的CF,在上午饲喂后4.5~10.0 h期间有降低血浆Kyn含量的趋势,对血浆Trp、5-HT、MT含量整体没有显著影响,但可提高绵羊血浆抗氧化能力。

本文引用格式

王根, 陈晖, 赵芳, 高超, 赵国栋, 李晓斌, 马晨, 杨开伦 . 瘤胃保护性色氨酸饲粮中添加亚叶酸钙对绵羊血浆色氨酸、犬尿氨酸和褪黑素含量的影响[J]. 动物营养学报, 2018 , 30(10) : 3968 -3975 . DOI: 10.3969/j.issn.1006-267x.2018.10.021

Abstract

The objective of this study was to explore the method of regulating the synthesis of melatonin (MT) in ruminants by detecting the effects of adding two doses of calcium folate (CF) in rumen protected trytophan (RPTrp) diets on plasma tryptophan (Trp), kynurenine (Kyn) and MT contents in sheep. Fifteen Suffolk sheep aged (3.0±0.5) years, with an average body weight (64.45±2.41) kg were divided into 3 groups (5 in each group):control group, trail group Ⅰ and trial group Ⅱ. The amount of feed (per day per sheep) were 12 g/kg BW of concentrate supplement, 1.8 kg corn silage, 222.2 mg/kg BW of RPTrp, and mixed hay was fed ad libitum. On the basis of this, sheep in trail group Ⅰ and trial group Ⅱ were fed 50 and 100 mg CF per sheep per day, respectively. The results showed as follows:1) there was no significant difference in plasma Trp and Kyn contents among groups during 0 to 12.0 h after feeding in the morning (P>0.05), but plasma Trp content in trial groups reduced at 6.0 and 8.0 h after feeding in the morning (P=0.087 2, P=0.053 1). Plasma Kyn content in the plasma of trial groups reduced too at 4.5, 6.0, 8.0 and 10.0 h after feeding in the morning (P=0.094 8, P=0.066 7, P=0.090 9, P=0.0542). 2) At 4.5 and 8.0 h after feeding in the morning, plasma 5-hydroxytryptamin (5-HT) content in trail groups increased compared with the control group (P=0.080 7, P=0.054 1). At 10.0 h after feeding in the morning, plasma 5-HT content in trail groups significantly increased (P=0.005 7). At 6.0 and 8.0 h after feeding in the morning, plasma MT content in trail groups also increased (P=0.089 0, P=0.070 4), and it was significantly higher at 10.0 h after feeding in the morning than that in the control group (P=0.000 2). 3) Compared with the control group, the plasma total antioxidant capacity and glutathione peroxidase activity in trail group Ⅱ were significantly increased (P<0.05), malonaldehyde content was significantly decreased (P<0.05), and total antioxidant capacity in trail group Ⅰ was also significantly improved (P<0.05). In conclusion, the RPTrp (222.2 mg/kg BW) diets with 50 or 100 mg CF per sheep per day has the tendency of reducing plasma Kyn content from 4.5 to 10.0 h after feeding in the morning, has no significant effect on plasma Trp, 5-HT, MT contents, but can improve the plasma antioxidant capacity of sheep.

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