分子营养 Molecular Nutrition

表皮生长因子对脂多糖刺激断奶仔猪肠道钠磷转运载体蛋白Ⅱb表达的影响

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  • 湖南农业大学动物科学技术学院, 湖南畜禽安全生产协同创新中心, 长沙 410128
汤小朋(1986-),男,湖南邵阳人,博士研究生,从事动物营养与饲料研究。E-mail:tangxiaopeng110@126.com

收稿日期: 2018-03-29

  网络出版日期: 2018-10-20

基金资助

湖南省自然基金面上项目(2018JJ2163);国家自然科学基金面上项目(31572419);湖南研究生科研创新项目(CX2016B276)

Effects of Epidermal Growth Factor on Intestinal Type Ⅱb Sodium-Phosphate Cotransporter Expression of Weaned Piglets Challenged by Lipopolysaccharide

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  • College of Animal Science and Technology, Hunan Agricultural University, Hunan Co-Innovation Center of Animal Production Safety, Changsha 410128, China

Received date: 2018-03-29

  Online published: 2018-10-20

摘要

本试验旨在研究表皮生长因子(EGF)对脂多糖(LPS)刺激的应激状态下仔猪肠道钠磷转运载体蛋白Ⅱb(NaPi-Ⅱb)表达的影响。本试验由2部分组成,1)细胞试验:以猪小肠上皮细胞(IPEC-J2)为模型,试验设4个组,对照组(0 ng/mL EGF,0 μg/mL LPS)、EGF组(100 ng/mL EGF,0 μg/mL LPS)、LPS组(0 ng/mL EGF,1.0 μg/mL LPS)、EGF+LPS组(100 ng/mL EGF,1.0 μg/mL LPS),每组3个重复;2)动物试验:选取24头体重接近、健康状况良好的21日龄"长白×大白"二元杂交断奶阉公猪[平均体重(5.76±0.38)kg],随机分为4个组,对照组(基础饲粮)、EGF组(基础饲粮+2 mg/kg EGF)、LPS组(基础饲粮+腹腔注射100 μg/kg BW LPS)、EGF+LPS组(基础饲粮+2 mg/kg EGF+腹腔注射100 μg/kg BW LPS),每组6个重复,每个重复1头猪。结果表明:1)细胞试验中,与对照组相比,EGF组IPEC-J2细胞NaPi-Ⅱb mRNA及蛋白表达显著下降(P<0.05),而EGF+LPS组细胞NaPi-Ⅱb mRNA及蛋白表达显著增加(P<0.05),EGF与LPS免疫应激的互作效应对NaPi-Ⅱb mRNA及蛋白表达影响显著(P<0.05)。2)动物试验中,各组间血清钙(Ca)含量无显著差异(P>0.05),LPS组血清磷(P)含量显著高于对照组、EGF组、EGF+LPS组(P<0.05),EGF组血清碱性磷酸酶(ALP)活性显著高于LPS组(P<0.05),EGF与LPS免疫应激互作效应对血清P含量影响显著(P<0.05),对血清Ca含量和ALP活性影响不显著(P>0.05)。与对照组相比,EGF组空肠与回肠NaPi-Ⅱb mRNA表达显著下降(P<0.05),而EGF+LPS组空肠与回肠NaPi-Ⅱb mRNA表达显著增加(P<0.05),EGF与LPS免疫应激互作效应对空肠与回肠NaPi-Ⅱb mRNA表达影响显著(P<0.05)。细胞与动物试验结果表明,EGF对NaPi-Ⅱb的表达起抑制作用,但在免疫应激状态下可促进NaPi-Ⅱb的表达,表明EGF可促进应激状态下肠道P的主动转运。

本文引用格式

汤小朋, 徐荣, 李成良, 彭鹏, 禹琪芳, 方热军 . 表皮生长因子对脂多糖刺激断奶仔猪肠道钠磷转运载体蛋白Ⅱb表达的影响[J]. 动物营养学报, 2018 , 30(10) : 4020 -4027 . DOI: 10.3969/j.issn.1006-267x.2018.10.027

Abstract

The aim of this experiment was to study the effects of epidermal growth factor (EGF) on intestinal type Ⅱb sodium-phosphate cotransporter (NaPi-Ⅱb) expression of weaned piglets under stress condition challenged by lipopolysaccharide (LPS). The study was consisted of two parts, 1) cell experiment:intestinal porcine intestinal epithelial cells (IPEC-J2) were as the experimental model, and divided into 4 groups with 3 replicates in each, control group (0 ng/mL EGF, 0 μg/mL LPS), EGF group (100 ng/mL EGF, 0 μg/mL LPS), LPS group (0 ng/mL EGF, 1.0 μg/mL LPS), EGF+LPS group (100 ng/mL EGF, 1.0 μg/mL LPS); 2) animal experiment:twenty-four 21-day-old healthy weaned piglets (Large White×Landrace) with an average body weight of (5.76±0.38) kg were randomly divided into 4 groups, control group (basal diet), EGF group (basal diet+2.0 mg/kg EGF), LPS group (basal diet+intraperitoneal injection of 100 mg/kg LPS), EGF+LPS group (basal diet+2.0 mg/kg EGF+intraperitoneal injection of 100 mg/kg LPS), with 6 replicates per group and 1 piglet in each replicate. The results showed as follows:1) in the cell experiment, compared with the control group, the expression of NaPi-Ⅱb mRNA and protein in IPEC-J2 cells was significantly decreased in EGF group (P<0.05), whereas, that in IPEC-J2 cells was significantly increased in EGF+LPS group (P<0.05), and there was a significant interaction between EGF and LPS immunological stress on the expression of NaPi-Ⅱb mRNA and protein (P<0.05); 2) in the animal experiment, there was no significant difference in serum Ca content among all groups (P>0.05), serum P content in LPS group was significantly higher than that in control group, EGF group, and EGF+LPS group (P<0.05), alkaline phosphatase (ALP) activity in EGF group was significantly higher than that in LPS group (P<0.05), there was a significant interaction between EGF and LPS immunological stress on serum P content (P<0.05), but not on Ca content and ALP activity (P>0.05). Compared with the control group, the expression of NaPi-Ⅱb mRNA in the jejunum and ileum in EGF group was significantly decreased (P<0.05), while, that in EGF+LPS group was significantly increased (P<0.05), and there was a significant interaction between EGF and LPS immunological stress on the expression of NaPi-Ⅱb mRNA in the jejunum and ileum (P<0.05). The results of cell experiment and animal experiment show that the EGF can inhibit the NaPi-Ⅱb expression, but can improve NaPi-Ⅱb expression under immunological stress condition, which indicate that EGF can promote the active absorption of P.

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