分子营养 Molecular Nutrition

瘤胃保护性5-羟基色氨酸对绵羊胃肠道内容物和血浆中5-羟基色氨酸、褪黑素含量的影响

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  • 新疆农业大学动物科学学院, 新疆肉乳用草食动物营养重点实验室, 乌鲁木齐 830052
赵芳(1989-),女,新疆阿勒泰人,博士研究生,研究方向为草食动物营养代谢。E-mail:937317612@qq.com

收稿日期: 2018-04-05

  网络出版日期: 2018-10-20

基金资助

国家自然科学基金项目(31760681)

Effects of Rumen Protected 5-Hydroxytryptophan on Contents of 5-Hydroxytryptophan, Melatonin in Gastrointestinal Tract Digesta and Plasma of Sheep

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  • Xinjiang Agricultural University, Xinjiang Key Laboratory of Meat & Milk Production Herbivore Nutrition, Urumqi 830052, China

Received date: 2018-04-05

  Online published: 2018-10-20

摘要

本试验旨在研究绵羊胃肠道内容物中褪黑素的分布特点,探究瘤胃保护性5-羟基色氨酸对绵羊胃肠道内容物和血浆中5-羟基色氨酸、褪黑素含量的影响,探讨通过5-羟基色氨酸调节绵羊肠道中褪黑素合成的可能性。试验选取健康、平均体重为(47.79±3.70)kg的3岁哈萨克母羊15只,按体重分为3组,每组5只,分别为对照组和试验Ⅰ、Ⅱ组。每天每只羊的粉状精料饲喂量为体重的1.2%,玉米青贮饲喂量为0.9 kg,混合干草自由采食,在此基础上,试验Ⅰ、Ⅱ组羊只分别饲喂111、222 mg/kg BW瘤胃保护性5-羟基色氨酸,进行15 d的饲养试验。结果表明:绵羊胃肠道内容物中5-羟基色氨酸含量的分布特点是盲肠 > 空肠、结肠 > 瘤胃液、十二指肠、回肠;褪黑素含量分布特点是十二指肠 > 瘤胃液、空肠 > 回肠、盲肠 > 结肠。除盲肠和结肠外,试验Ⅰ、Ⅱ组绵羊胃肠道内容物中5-羟基色氨酸含量极显著高于对照组(P<0.01)。试验Ⅱ组十二指肠内容物中5-羟色胺、N-乙酰-5-羟色胺含量极显著高于对照组(P<0.01),结肠内容物中5-羟色胺、N-乙酰-5-羟色胺含量显著高于对照组(P<0.05);试验Ⅰ组空肠、回肠内容物中5-羟色胺、N-乙酰-5-羟色胺含量极显著低于对照组、试验Ⅱ组(P<0.01)。试验Ⅰ、Ⅱ组结肠内容物中褪黑素含量极显著高于对照组(P<0.01)。试验Ⅰ、Ⅱ组十二指肠、空肠内容物中5-羟基吲哚乙酸含量极显著低于对照组(P<0.01),但试验Ⅱ组盲肠、结肠内容物中5-羟基吲哚乙酸含量极显著高于对照组(P<0.01)。试验Ⅱ组血浆中5-羟基色氨酸、5-羟色胺、褪黑素含量均显著或极显著高于对照组(P<0.05或P<0.01)。由此可见,绵羊胃肠道内容物中5-羟基色氨酸含量的分布特点是:盲肠 > 空肠、结肠 > 瘤胃液、十二指肠、回肠;褪黑素含量分布特点是:十二指肠 > 瘤胃液、空肠 > 回肠、盲肠 > 结肠。瘤胃保护性5-羟基色氨酸补喂量为222 mg/kg BW时能提高胃肠道内容物中5-羟基色氨酸含量,但对5-羟色胺、褪黑素含量的影响在各胃肠段表现不同。瘤胃保护性5-羟基色氨酸补喂量为111、222 mg/kg BW时均能提高绵羊血浆中5-羟基色氨酸、5-羟色胺、褪黑素含量。

本文引用格式

赵芳, 陈晖, 王根, 高超, 赵国栋, 马晨, 杨开伦 . 瘤胃保护性5-羟基色氨酸对绵羊胃肠道内容物和血浆中5-羟基色氨酸、褪黑素含量的影响[J]. 动物营养学报, 2018 , 30(10) : 4037 -4047 . DOI: 10.3969/j.issn.1006-267x.2018.10.029

Abstract

This study aimed to investigate the distribution of melatonin in gastrointestinal tract digesta of sheep, to study the effects of rumen protected 5-hydroxytryptophan (5-HTP) on contents of 5-HTP, melatonin in gastrointestinal tract digesta and plasma of sheep, and to explore the possibility of regulating sheep gastrointestinal tract melatonin synthesis by 5-HTP. Fifteen 3-year-old Kazak sheep with an average body weight of (47.79±3.70) kg were divided into 3 groups (control group, trial group Ⅰ and trial group Ⅱ) with 3 sheep per group. All sheep were fed 1.2% body weight powder concentrate, and 0.9 kg corn silage per sheep per day, and had free feeding mixed with hay, additionally, each sheep in trail groups Ⅰ and Ⅱ was fed 111 and 222 mg/kg BW rumen protected 5-HTP, respectively. The feeding experiment lasted for 15 days. The results showed that the distribution characteristics of the content of 5-HTP in gastrointestinal tract digesta of sheep were:caecum > jejunum, colon > rumen fluid, duodenum, ileum; and the distribution characteristics of melatonin content were:duodenum > rumen fluid, jejunum > ileum, caecum > colon. Expect caecum and colon, the content of 5-HTP in gastrointestinal tract digesta of sheep in trail groups Ⅰ and Ⅱ was significantly higher than that in the control group (P<0.01). The contents of 5-hydroxytryptamine (5-HT) and N-acetylserotonin (NAS) in duodenum digesta in trail group Ⅱ were significantly higher than those in the control group (P<0.01), and the contents of 5-HT and NAS in colon digesta in trail group Ⅱ were significantly higher than those in the control group (P<0.05); the contents of 5-HT and NAS in jejunum and ileum digesta in trial group Ⅰ were significantly lower than those in the control group and trial group Ⅱ (P<0.01). The content of melatonin in colon digesta in trail groups Ⅰ and Ⅱ was significantly higher than that in the control group (P<0.01). The content of 5-hydroxyindoleaceticacid (5-HIAA) in duodenum and jejunum digesta in trail groups Ⅰ and Ⅱ was significantly lower than that in the control group (P<0.01), however, the content of 5-HIAA in caecum and colon digesta in trial group Ⅱ was significantly higher than that in the control group (P<0.01). The contents of 5-HTP, 5-HT and melatonin in plasma in trial group Ⅱ were significantly higher than those in the control group (P<0.05 or P<0.01). Therefore, the distribution characteristics of the 5-HTP content in gastrointestinal tract digesta of sheep are:caecum > jejunum, colon > rumen fluid, duodenum, ileum, and the distribution characteristics of melatonin content are:duodenum > rumen fluid, jejunum > ileum, caecum > colon. Rumen protected 5-HTP supplementation at 222 mg/kg BW can increase the content of 5-HTP in gastrointestinal tract digesta, but its effect on 5-HT and melatonin contents perform differently in different gastrointestinal tract. Rumen protected 5-HTP supplementation at 111 and 222 mg/kg BW can increase the contents of 5-HTP, 5-HT and melatonin in plasma of sheep.

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