动物营养学报 >

2018 , Vol. 30 >Issue 10: 4048 - 4058

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.10.030

分子营养 Molecular Nutrition

基于16S rDNA高通量测序分析技术研究甘露寡糖对奶牛瘤胃菌群结构的影响

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  • 宁夏大学农学院, 银川 750021
郭婷婷(1993-),女,山东菏泽人,硕士研究生,研究方向为反刍动物营养。E-mail:guoyx729@163.com

收稿日期: 2018-03-14

  网络出版日期: 2018-10-20

基金资助

国家自然科学基金项目（31460619）

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Effects of Mannan Oligosaccharides on Rumen Microflora Structure of Dairy Cows Based on 16S rDNA High-Throughput Sequencing Technology

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  • Collage of Agriculture, Ningxia University, Yinchuan 750021, China

Received date: 2018-03-14

  Online published: 2018-10-20

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摘要

本试验旨在利用16S rDNA高通量测序分析技术研究甘露寡糖对奶牛瘤胃菌群结构的影响。选择泌乳阶段相近、胎次相同的泌乳早期奶牛4头，随机分为2组，对照组饲喂基础饲粮，试验组饲喂基础饲粮，同时添加60 g/头甘露寡糖，通过口腔灌注添加。采用2×2交叉试验设计，每期21 d，预试期14 d，采样期7 d。结果表明：1）与对照组相比，添加甘露寡糖极显著提高了瘤胃液乙酸的浓度（P<0.01），乙酸/丙酸提高了8.47%（P>0.05）。2）与对照组相比，门水平上，试验组蓝藻门相对丰度极显著降低（P<0.01），装甲菌门的相对丰度极显著提高（P<0.01）；属水平上，试验组厌氧螺菌属的相对丰度显著提高（P<0.05），梭菌属的相对丰度显著降低（P<0.05），锥形杆菌属的相对丰度极显著降低（P<0.01），瘤胃球菌属、假丁酸弧菌属以及毛螺菌属的相对丰度分别增加24.34%、12.83%、31.80%（P>0.05），普雷沃氏菌属的相对丰度降低4.66%（P>0.05），未注释韦荣氏菌属的相对丰度提高143.11%（P>0.05），脱硫弧菌属的相对丰度提高4.88%（P>0.05）。在本试验条件下，添加甘露寡糖降低了瘤胃细菌的多样性，对纤维素降解菌群、半纤维素降解菌群影响明显，显著提高了瘤胃液乙酸浓度，提高了瘤胃液pH，但差异不显著。

关键词： 奶牛; 瘤胃; 甘露寡糖; 16S rDNA; 菌群

本文引用格式

郭婷婷, 胡丹丹, 付子琳, 李娜, 徐晓锋 . 基于16S rDNA高通量测序分析技术研究甘露寡糖对奶牛瘤胃菌群结构的影响[J]. 动物营养学报, 2018 , 30(10) : 4048 -4058 . DOI: 10.3969/j.issn.1006-267x.2018.10.030

Abstract

This experiment was conducted to study the effects of mannan oligosaccharides on rumen microflora structure of dairy cows based on 16S rDNA high-throughput sequencing technology. Four healthy Chinese Holstein lactating dairy cows with the same parity in the similar lactation period were randomly divided into 2 groups. Cows in control group were fed a basal diet and those in experimental group were fed the basal diet with 60 g/cow mannan oligosaccharides through oral perfusion. The experiment was designed through a 2×2 cross-over test with 21 days in each stage (14 days for pre-experiment and 7 days for samples collection). The results showed as follows:1) compared with the control group, adding mannose oligosaccharides significantly increased acetic acid concentration in the rumen (P<0.01), and acetic acid/propionic acid also increased by 8.47% (P>0.05). 2) Compared with the control group, at the phylum level, the relative abundance of Cyanobacteria in experimental group decreased significantly (P<0.01), and that of Armatimonadetes increased significantly (P<0.01). At the genus level, the relative abundance of Anaerobiospirillum in experimental group significantly increased (P<0.05), and that of Clostridium significantly decreased (P<0.05). The relative abundance of Pyramidobacter decreased significantly (P<0.01). The relative abundances of Ruminococcus, Pseudobutyrivibrio and Lachnospira increased by 24.34%, 12.83% and 31.80% (P>0.05), respectively. The relative abundance of Prevotella reduced by 4.66%, and that of Veillonellaceae_NA and Desulfovibrio increased by 143.11% and 4.88% (P>0.05), respectively, In this experiment, adding mannan oligosaccharide reduces the diversity of rumen bacteria of dairy cows, which obviously affects cellulolytic bacteria flora, hemicellulose-degrading bacteria flora and significantly increases the concentration of acetic acid in rumen fluid,and increases pH in rumen fluid, but has no significant effect.

Key words： dairy cows; rumen; mannan oligosaccharides; 16S rDNA; microflora

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