黄芪多糖和丁酸梭菌对蛋雏鸭免疫性能、抗氧化性能以及肠道形态的影响

赵文文, 袁文华, 沈军达, 袁慧坤, 王源, 李国勤, 卢立志, 刁新平

doi:10.3969/j.issn.1006-267x.2018.10.040

动物营养学报 >

2018 , Vol. 30 >Issue 10: 4143 - 4150

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.10.040

饲料营养 Feed science and technology

黄芪多糖和丁酸梭菌对蛋雏鸭免疫性能、抗氧化性能以及肠道形态的影响

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1. 东北农业大学动物科学技术学院, 哈尔滨 150030;
2. 浙江省农业科学院畜牧兽医研究所, 杭州 310000;
3. 农业部农产品信息溯源重点实验室, 杭州 310021

赵文文(1992-),女,河南周口人,硕士研究生,动物营养与饲料科学专业。E-mail:zww0925wen@126.com

收稿日期: 2018-03-31

网络出版日期: 2018-10-20

基金资助

国家水禽产业技术体系杭州实验站项目（CARS-42-39）；浙江省农业（畜禽）新品种选育重大科技专项（2016C02054-12）

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Effects of Astragalus Polysaccharides and Clostridium butyricum on Immune Function, Antioxidant Ability and Intestinal Morphology of Laying Ducklings

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1. College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China;
2. Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310000, China;
3. Key Laboratory of Information Traceability for Agricultural Products, Ministry of Agriculture of China, Hangzhou 310021, China

Received date: 2018-03-31

Online published: 2018-10-20

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摘要

本试验旨在研究饲粮中添加黄芪多糖、丁酸梭菌及其复合剂对蛋雏鸭免疫性能、抗氧化性能以及肠道形态的影响。选取1日龄健康绍兴公鸭600只，随机分为5组，每组6个重复，每个重复20只鸭。Ⅰ组（对照组）饲喂基础饲粮，Ⅱ~Ⅴ组分别在基础饲粮的基础上添加40 mg/kg的杆菌肽锌（抗生素组）、800 mg/kg的黄芪多糖、250 mg/kg的丁酸梭菌以及800 mg/kg的黄芪多糖+250 mg/kg的丁酸梭菌的复合剂。试验期为28 d。结果表明：1）Ⅴ组的血清免疫球蛋白A（IgA）含量显著高于Ⅰ组、Ⅱ组和Ⅳ组（P<0.05），Ⅴ组的血清免疫球蛋白G（IgG）及补体3（C3）、补体4（C4）的含量高于其他各组（P>0.05）。2）Ⅴ组的胸腺指数和脾脏指数均显著高于Ⅰ组和Ⅱ组（P<0.05），Ⅴ组的法氏囊指数与其他各组差异不显著（P>0.05）。3）Ⅲ组、Ⅳ组、Ⅴ组的血清和肝脏中的总抗氧化能力（T-AOC）显著高于Ⅰ组（P<0.05），血清和肝脏中的总超氧化物歧化酶（T-SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性均显著高于Ⅰ组和Ⅱ组（P<0.05），Ⅳ组的血清和肝脏中的丙二醛（MDA）含量显著低于Ⅰ组（P<0.05）。4）Ⅴ组的十二指肠、空肠和回肠的隐窝深度显著低于Ⅰ组和Ⅱ组（P<0.05），Ⅴ组的空肠和回肠的绒毛高度/隐窝深度值显著高于Ⅰ组和Ⅱ组（P<0.05）。由此可见，饲粮中添加黄芪多糖和丁酸梭菌复合剂比单独添加黄芪多糖或丁酸梭菌能更好地提高蛋雏鸭的免疫性能、抗氧化性能，改善肠道绒毛形态。

关键词： 黄芪多糖; 丁酸梭菌; 免疫; 抗氧化性能; 肠道形态

本文引用格式

赵文文, 袁文华, 沈军达, 袁慧坤, 王源, 李国勤, 卢立志, 刁新平 . 黄芪多糖和丁酸梭菌对蛋雏鸭免疫性能、抗氧化性能以及肠道形态的影响[J]. 动物营养学报, 2018 , 30(10) : 4143 -4150 . DOI: 10.3969/j.issn.1006-267x.2018.10.040

Abstract

This experiment was designed to investigate the effects of Astragalus polysaccharides, Clostridium butyricum and their compound agent on immune function, antioxidant ability and intestinal morphology of laying ducklings. A total of 600 one-day-old healthy Shaoxing male ducklings were randomly assigned to 5 groups with 6 replicates per group and 20 ducklings per replicate. Ducklings in the group Ⅰ (control group) were fed a basal diet, while others in groups Ⅱ to Ⅴ were fed the basal diet supplemented with 40 mg/kg Bacitracin (antibiotic group), 800 mg/kg Astragalus polysaccharides, 250 mg/kg Clostridium butyricum and 800 mg/kg Astragalus polysaccharides+250 mg/kg Clostridium butyricum, respectively. The experiment lasted for 28 days. The results showed as follows:1) the serum immunoglobulin A (IgA) content in group Ⅴ was significantly higher than that groups Ⅰ, Ⅱ and Ⅳ (P<0.05). The contents of immunoglobulin G (IgG), complement 3 (C3) and complement 4 (C4) in serum in group Ⅴ were higher than those in other groups (P>0.05). 2) The thymus index and spleen index in group Ⅴ were significantly higher than those in groups Ⅰ and Ⅱ (P<0.05), the bursa of Fabricius index in group Ⅴ had no significant different compared with other groups (P>0.05). 3) The total antioxidant capacity (T-AOC) in serum and liver in groups Ⅲ, Ⅳ and Ⅴ was significantly higher than that in group Ⅰ (P<0.05), and the activities of total superoxide dismutase (T-SOD), and glutathione peroxidase (GSH-Px) in serum and liver in groups Ⅲ, Ⅳ and Ⅴ were significantly higher than those in groups Ⅰ and Ⅱ (P<0.05), the content of malondialdehyde (MDA) in serum and liver in group Ⅳ was significantly lower than that in group Ⅰ (P<0.05). 4) The crypt depth in duodenum, jejunum and ileum in group Ⅴ was significantly lower than that in groups Ⅰ and Ⅱ (P<0.05), the villous height to crypt depth value in jejunum and ileum was significantly higher than that in groups Ⅰ and Ⅱ (P<0.05). It is concluded that the addition of Astragalus polysaccharides and Clostridium butyricum in diets can improve the immune function and antioxidant ability of laying duckling, which is better than the single addition of Astragalus polysaccharides or Clostridium butyricum, and improve intestinal villus morphology.

Key words： Astragalus polysaccharides; Clostridium butyricum; immune; antioxidant ability; intestinal morphology

参考文献

[1] 辛娜,刁其玉,张乃锋.芽孢杆菌在动物营养与饲料中的应用[J].中国饲料,2010(14):26-29.

[2] 王润之,黄艳群,陈文,等.酪酸菌在家禽生产中的应用[J].饲料研究,2010(6):22-23.

[3] 郑璇,郑育洪.国内外超级细菌的研究进展及防控措施[J].中国畜牧兽医文摘,2012(1):69-75.

[4] 柳志余.黄芪多糖对珍珠鸡生长性能、免疫功能和血液指标的影响[J].黑龙江畜牧兽医,2013(7):130-132.

[5] CHEN H L,LI D F,CHANG B Y,et al.Effects of Chinese herbal polysaccharides on the immunity and growth performance of young broilers[J].Poultry Science,2003,82(3):364-370.

[6] 肖娅楠.黄芪多糖及其在肉仔鸡生产中的应用[J].山东畜牧兽医,2013(4):81-82.

[7] NEWBOLD C J,WALLACE R J,MCLNTOSH F M.Mode of action of the yeast Saccharomyces cerevisiae as a feed additive for ruminants[J].British Journal of Nutrition,1996,76(2):249-261.

[8] 刘阳,启航,闫晓梅.中药黄芪有效成分对益生菌的促生作用[J].大连轻工业学院学报,2005,24(3):192-194.

[9] 岑路,陈蕾,张小利,等.益生菌和黄芪多糖临床改善仔猪生长性能的研究[J].中国畜牧兽医,2009,36(12):17-19.

[10] 易力,倪学勤, 潘康成,等.微生态制剂对仔鸡生产性能和免疫功能的影响[J].中国家禽,2004,26(23):10-13.

[11] LIN J,KANG H,LIANG J,et al.CpG oligonucleotides and Astragalus polysaccharides are effective adjuvants in cultures of avian bone-marrow-derived dendritic cells[J].British Poultry Science,2015,56(1):30-38.

[12] 王海凤,郭兵.黄芪多糖对肉仔鸡十二指肠黏膜的免疫调节作用[J].动物营养学报,2015,27(5):1534-1539.

[13] YANG C M,CAO G T,FERKET P R,et al.Effects of probiotic,Clostridium butyricum,on growth performance,immune function,and cecal microflora in broiler chickens[J].Poultry Science,2012,91(9):2121-2129.

[14] 郑有秀,王超,邹晓庭,等.酸梭菌对断奶仔猪生长性能、肠道结构和免疫功能的影响[J].动物营养学报,2018,30(7):2683-2689.

[15] 邹知明,梁龙华,崔涵,等.功能性氨基酸与黄芪多糖、益生素的互作对仔猪肠道微生态环境和肠道健康的影响[J].饲料工业,2016,37(21):35-39.

[16] 黄克宏,游纯波,周学光,等.苏氨酸和黄芪多糖、益生素的互作对仔猪免疫机能的影响及其机理研究[J].黑龙江畜牧兽医,2015(23):122-125.

[17] 王虹玲,刘丹丹,姜诗文,等.复合微生态制剂与黄芪多糖对肉鸡生长性能、肠道菌群和免疫功能的影响[J].饲料工业,2014,35(6):10-14.

[18] 林显华,孙合美,谷巍,等.枯草芽孢杆菌B7对肉鸡生长性能及免疫性能的影响[J].中国畜牧兽医,2013,40(2):91-95.

[19] 苏兰利,王思敏,姜雨桦,等.黄芪多糖浸泡种蛋对雏鸡免疫器官抗氧化性能的影响[J].黑龙江畜牧兽医,2014(11):157-159.

[20] 王俊丽,章世元,徐春燕,等.黄芪多糖对肉仔鸡生产性能和部分免疫指标的影响[J].中国饲料,2010(7):18-21.

[21] 贾志新.丁酸梭菌对樱桃谷肉鸭生长性能、免疫和抗氧化功能及肠道食糜VFA含量的影响[D].硕士学位论文.南京:南京农业大学,2014.

[22] 吕鑫.黄芪多糖和益生菌对海兰褐蛋鸡生产性能和免疫功能的影响[J].饲料博览,2014(12):24-28.

[23] 赵云焕,李迎晓,焦凤超,等.黄芪多糖、益生菌对固始鸡生产性能和免疫效果的影响[J].江苏农业科学,2012,40(9):202-203.

[24] 杨维军,王华,杨坚.益生菌的功效及其在食品中的应用[J].四川食品与发酵,2005,41(1):27-30.

[25] 马森.谷胱甘肽过氧化物酶和谷胱甘肽转硫酶研究进展[J].动物医学进展,2008,29(10):53-56.

[26] 王咏梅.自由基与谷胱甘肽过氧化物酶[J].解放军药学学报,2005,21(5):369-371.

[27] 孙波,陈静,刘江,等.饲料中添加黄芪多糖对肉鸡肠道菌群及免疫器官指数的影响[J].黑龙江畜牧兽医,2014(13):86-88.

[28] 陶浩.黄芪多糖对肉仔鸡肠道发育及抗氧化能力的影响[D].硕士学位论文.长春:吉林农业大学,2012.

[29] 左兆云,杨维仁,杨在宾,等.日粮添加黄芪多糖对蛋鸡机体抗氧化能力和鸡蛋品质的影响[J].中国兽医学报,2012,32(1):130-134.

[30] 郑嫩珠,李丽,辛清武,等.酵母硒、黄芪多糖及其复合剂对半番鸭生长性能、肉品质和抗氧化能力的影响[J].动物营养学报,2016,28(12):3856-3866.

[31] 赵熙,冉陆,杨宝兰,等.丁酸梭菌活菌制剂对肠道菌群影响的研究[J].中国微生态学杂志,1999,11(6):332-333.

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