动物营养学报 >

2018 , Vol. 30 >Issue 10: 4210 - 4218

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.10.047

饲料资源开发 Feed Resources Development

大黑山薏苡草粉替代苜蓿草粉对生长肉兔肠道形态、消化酶活性及盲肠发酵参数的影响

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  • 四川农业大学动物营养研究所, 动物抗病营养教育部重点实验室, 成都 611130
田刚(1974-),男,重庆人,副教授,博士,主要从事家兔营养与饲料高效利用研究。E-mail:tgang2008@126.com

收稿日期: 2018-03-20

  网络出版日期: 2018-10-20

基金资助

四川省科技支撑计划项目（2016NZ0002）；四川农业大学"双支计划"项目

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Effects of Dietary Replacement of Alfalfa Meal by Coix lacryma-jobi cv. Daheishan Meal on Intestinal Morphology, Digestive Enzyme Activities and Cecal Fermentation Parameters of Growing Meat Rabbits

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  • Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2018-03-20

  Online published: 2018-10-20

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摘要

本试验旨在研究饲粮中不同比例大黑山薏苡草粉替代苜蓿草粉对生长肉兔肠道形态、消化酶活性及盲肠发酵参数的影响。试验选取体重接近的35日龄断奶新西兰白兔100只，随机分为5组，每组10个重复，每个重复2只兔。对照组（C组）和4个替代组（S25、S50、S75和S100组）分别饲喂5种不同比例（0、25%、50%、75%和100%）大黑山薏苡草粉替代苜蓿草粉的试验饲粮。试验期15 d。试验第15天早晨空腹称重后，从各组不同重复中选取4只接近平均体重的试验兔，屠宰取小肠和盲肠组织、黏膜和食糜样品用于分析。结果表明：各组间平均日采食量、平均日增重和料重比均无显著差异（P>0.05）。各组间小肠各段的绒毛高度、隐窝深度、绒毛高度与隐窝深度比值及盲肠隐窝深度均无显著差异（P>0.05）。S50组十二指肠中蔗糖酶活性显著高于S75和S100组（P<0.05），而各组间其他各肠道各消化酶活性均无显著差异（P>0.05），但均以S50组活性较高。各组间盲肠pH和氨态氮、总挥发性脂肪酸、乙酸、丙酸、丁酸含量以及乙酸/（丙酸+丁酸）均无显著差异（P>0.05）。基于16S rRNA二代测序结果发现，C组与S100组盲肠微生物在门水平及在属水平上的组成均无明显差异。由此可见，大黑山薏苡草粉替代饲粮中苜蓿草粉不影响生长肉兔肠道形态、消化酶活性及盲肠发酵指标，大黑山薏苡草粉可完全替代生长肉兔饲粮中的苜蓿草粉，但推荐替代比例为50%。

关键词： 大黑山薏苡草粉; 生长肉兔; 肠道形态; 肠道酶活性; 盲肠发酵

本文引用格式

田刚, 鲁院院, 余冰, 曾绘锦, 陈代文, 罗玉衡, 蔡景义 . 大黑山薏苡草粉替代苜蓿草粉对生长肉兔肠道形态、消化酶活性及盲肠发酵参数的影响[J]. 动物营养学报, 2018 , 30(10) : 4210 -4218 . DOI: 10.3969/j.issn.1006-267x.2018.10.047

Abstract

This experiment was conducted to investigate the effects of dietary different proportions Coixies lacryma-jobi cv. Daheishan meal replacement alfalfa meal on intestinal morphology, digestive enzyme activitives and cecal fermentation parameters of growing meat rabbits. A total of 100 weaned New Zealand white rabbits (35-day-old) with a similar weight were randomly assigned to 5 groups with 10 replicates per group and 2 rabbits per replicate. Rabbits in the control group (C group) and 4 replacement groups (S25, S50, S75 and S100 groups) were fed 5 experimental diets with different proportions (0, 25%, 50%, 75% and 100%) of alfalfa meal replaced by Coix lacryma-jobi cv. Daheishan meal. The experiment lasted for 15 days. On the 15th day of the experiment, 4 rabbits with an average body weight from each group were selected and slaughtered, and the tissue, mucous and digesta samples in small intestine and cecum were collected immediately for further analysis. The results indicated that there were no significant differences in the average daily intake, average daily gain and feed to gain ratio among all the groups (P>0.05). There were no significant differences in the villus height, crypt depth and the ratio of villous height to crypt depth in small intestine and crypt depth in cecum among all the groups (P>0.05). The duodenum sucrase activity in S50 group was significantly higher than that in S75 and S100 groups (P<0.05), however, there were no significant differences in the activities of other digestive enzymes in other intestinal tract among all groups (P>0.05), and the digestive enzyme activity was the highest in S50 group. There were no significant differences in the pH and contents of ammonia nitrogen, total volatile fatty acids, acetic acid, propionic acid and butyric acid, and acetic acid/(propionic acid+butyric acid) in cecum among all the groups (P>0.05). According to the results of 16S rRNA sequencing, the diversity of cecal microorganisms showed no difference at the phylum level and genus level between C group and S100 group. The results indicated that the dietary alfalfa meal replaced by the Coixlacryma-jobi cv. Daheishan meal has no significant effects on intestinal morphology, digestive enzyme activity and cecal fermentation parameters of growing meat rabbits, and the dietary alfalfa meal can be completely replaced by Coixlacryma-jobi cv. Daheishan meal, and the recommended substitution ratio is 50%.

Key words： Coix lacryma-jobi cv. Daheishan meal; growing meat rabbits; intestinal morphology; digestive enzyme activity; caecum fermentation

参考文献

[1] GIDENNE T.Dietary fibres in the nutrition of the growing rabbit and recommendations to preserve digestive health:a review[J].Animal,2015,9(2):227-242.  

[2] 王熙遥.中国苜蓿市场供给和需求关系研究[D].硕士学位论文.呼和浩特:内蒙古大学,2017:12-16.

[3] 赵晓明.薏苡[M].北京:中国林业出版社,2000:1-21.

[4] LIM T K.Coix lachryma-jobi[M].Netherlands:Springer,2013:243-261.

[5] 国家药典委员会.中华人民共和国药典[M].北京:化学工业出版社,2010:353-354.

[6] NISHIMURA M,OHKAWARA T,KAGAMI-KATSUYAMA H,et al.Alteration of intestinal flora by the intake of enzymatic degradation products of adlay (Coix lachryma-jobi,L.var.ma-yuen,Stapf) with improvement of skin condition[J].Journal of Functional Foods,2014,7:487-494.

[7] CHUNG C P,HSIA S M,LEE M Y,et al.Gastroprotective activities of Adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) on the growth of the stomach cancer AGS cell line and indomethacin-induced gastric ulcers[J].Journal of Agricultural and Food Chemistry,2011,59(11):6025-6033.  

[8] 张明发,沈雅琴.薏苡仁油抗消化系肿瘤的基础和临床研究[J].中国执业药师,2011,8(8):19-23.

[9] 李艳玲,齐玉梅.添加薏苡仁的肠内营养对脑卒中患者肠屏障功能的影响[J].卫生研究,2011,40(4):503-504.

[10] 朱自平,王红武,张明发,等.薏苡仁的消化系统药理研究[J].现代中药研究与实践,1998(4):36-38.

[11] CHIANG W,CHENG C Y,CHIANG M T,et al.Effects of dehulled adlay on the culture count of some microbiota and their metabolism in the gastrointestinal tract of rats.[J].Journal of Agricultural and Food Chemistry,2000,48(3):829-832.  

[12] 李爽,沈晓庆,章琦,等.薏苡仁及其麸炒品对动物胃肠动力的影响[J].亚太传统医药,2012,8(4):29-32.

[13] WANG C Y,LIN H T,WU S C.Influence of dietary supplementation with Bacillus-fermented adlay on lipid metabolism,antioxidant status and intestinal microflora in hamsters[J].Journal of the Science of Food & Agriculture,2011,91(12):2271-2276.

[14] BAO C,ZENG H L,ZHANG Y,et al.Structural characteristics of semen coicis resistant starch and its effect on the proliferation of Bifidobacterium bifidum[J].Chinese Journal of Structural Chemistry,2017,36(3):511-521.

[15] 易醒,侯海涛,吴达成,等.在体单向灌流法研究W/O/W型薏苡仁油复乳的大鼠肠吸收特性[J].南昌大学学报(理科版),2016,40(3):244-249.

[16] 杨宝贵.薏仁米秸杆饲料化利用前景分析[J].经济管理(文摘版),2016(3):323.

[17] 鲁院院,田刚,余冰,等.晒干大黑山薏苡全株在生长肉兔上的营养价值评定[J].草业科学,2017,34(5):1100-1106.

[18] 翁长江.薏苡副产品对兔及猪生长性能的影响[J].饲料博览,2013(10):37-39.

[19] 田刚,鲁院院,余冰,等.不同比例大黑山薏苡草粉饲粮对生长兔生产性能、养分消化率和屠宰性能的影响[J].动物营养学报,2018,30(5):1928-1935.

[20] BLASCO A,OUHAYOUN J,MASOERO G.Status of rabbit meat and carcass:criteria and terminology[J].Options Méditerranéennes-Série A:Séminaires,1992:105-120.

[21] SPILLER G A,CHERNOFF M C,HILL R A,et al.Effect of purified cellulose,pectin,and a low-residue diet on fecal volatile fatty acids,transit time,and fecal weight in humans[J].The American Journal of Clinical Nutrition,1980,33(4):754-759.  

[22] WEATHERBURN M W.Phenol-hypochlorite reaction for determination of ammonia[J].Analytical Chemistry,1967,39(8):971-974.  

[23] CASPARY W F.Physiology and pathophysiology of intestinal absorption[J].The American Journal of Clinical Nutrition,1992,55(1):299S-308S.

[24] DE BLAS C,GARCÍA J,CARABAÑO R.Role of fibre in rabbit diets.A review[J].Annales De Zootechnie,1999,48(1):3-13.  

[25] CHIOU P W S,YU B,LIN C.Effect of different components of dietary fiber on the intestinal morphology of domestic rabbits[J].Comparative Biochemistry and Physiology Part A:Physiology,1994,108(4):629-638.  

[26] 吕惠敏.复方中草药有效成分的分析及对兔肠道功能的影响[D].硕士学位论文.福建:福建农林大学,2012:44-50.

[27] HA D T,NAM T T,BICH T N,et al.Adlay seed extract (Coix lachryma-jobi L.) decreased adipocyte differentiation and increased glucose uptake in 3T3-L1 cells[J].Journal of Medicinal Food,2010,13(6):1331-1339.  

[28] GRAY G M.Carbohydrate digestion and absorption-role of the small intestine[J].New England Journal of Medicine,1975,292(23):1225-1230.  

[29] 张云刚,陈安国.小肠黏膜二糖酶的研究进展[J].饲料工业,2003,24(3):16-18.

[30] DE BLAS C,WISEMAN J.Nutrition of the rabbit[Z].CABI,2010.

[31] CALVERT R,SCHNEEMAN B O,SATCHITHANANDAM S,et al.Dietary fiber and intestinal adaptation:effects on intestinal and pancreatic digestive enzyme activities[J].The American Journal of Clinical Nutrition,1985,41(6):1249-1256.  

[32] GIDENNE T,JEHL N,SEGURA M,et al.Microbial activity in the caecum of the rabbit around weaning:impact of a dietary fibre deficiency and of intake level[J].Animal Feed Science and Technology,2002,99(1/2/3/4):107-118.

[33] 刘晓雯,刘克武,江琰,等.部分中药材及调味料对小肠蔗糖酶活性的影响[J].中国生化药物杂志,2003,24(5):229-232.

[34] UNI Z,NOY Y,SKLAN D.Posthatch development of small intestinal function in the poult[J].Poultry Science,1999,78(2):215-222.  

[35] SIDDONS R C.Effect of diet on disaccharidase activity in the chick[J].British Journal of Nutrition,1972,27(2):343-352.  

[36] 晁洪雨,李福昌.日粮ADF水平对生长肉兔氮平衡、免疫及消化酶活性的影响[J].中国饲料,2007(15):18-20,23.

[37] GIDENNE T.The caecal ecosystem in the growing rabbit:impact of nutritional and feeding factors[J].Rabbit Congress of the America,2006(3):1-17.

[38] 刘方正.干苹果渣对断奶幼兔生长性能和盲肠内环境的影响[D].硕士学位论文.杨凌:西北农林科技大学,2011:23-26.

[39] GARCÍA J,GIDENNE T,FALCAO-E-CUNHA L,et al.Identification of the main factors that influence caecal fermentation traits in growing rabbits[J].Animal Research,2002,51(2):165-173.  
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