动物营养学报 >

2018 , Vol. 30 >Issue 10: 4229 - 4236

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.10.049

研究简报 Short communications

沙子岭猪脂联素及其相关基因的mRNA和蛋白表达发育性变化规律

展开
  • 湖南农业大学动物科学技术学院, 湖南畜禽安全生产协同创新中心, 长沙 410001
陈宇光(1977-),男,湖南湘乡人,副教授,博士,动物生产与畜牧工程专业。E-mail:86851365@qq.com

收稿日期: 2018-04-06

  网络出版日期: 2018-10-20

基金资助

湖南省教育厅重点项目（13A035）；湖南省重点研发计划（2015NK2004，2015JC3100，2015NK1002）

收起

Developmental Changes on Related Gene and Protein Expression of Adiponectin of Shaziling Pigs

Expand
  • College of Animal Science and Technology, Hunan Agricultural University;Hunan Livestock Safety Production Cooperative Innovation Center, Changsha 410001, China

Received date: 2018-04-06

  Online published: 2018-10-20

Fold

摘要

本试验旨在对沙子岭猪不同组织中脂联素（ADIPOQ）、脂联素受体（ADIPOR）及其相关基因的mRNA和蛋白表达量的发育性变化规律进行研究。选取12头沙子岭猪（1、28、140日龄各4头），测定不同组织器官中ADIPOQ、ADIPOR1、ADIPOR2的mRNA和蛋白及2型拓扑异构酶β型（TOP2Β）、TATA结合蛋白（TBP）mRNA的发育性变化。结果表明：猪不同组织中ADIPOQ、ADIPOR1、ADIPOR2、TOP2β和TBP的mRNA表达量基本随着猪的生长而逐渐升高，140日龄时大部分组织器官中这几种基因的mRNA表达量显著或极显著高于1日龄（P<0.05或P<0.01）。大部分组织器官中ADIPOQ、ADIPOR1和ADIPOR2的蛋白表达量呈现出降低的趋势。由此可见，ADIPOQ及相关基因mRNA和蛋白的表达量与猪的脂肪沉积呈负相关，机体能根据自身需要对其进行调节。

关键词： 脂联素; 脂联素受体; 基因; 发育性变化; 沙子岭猪

本文引用格式

陈宇光, 秦龙山, 贺喜 . 沙子岭猪脂联素及其相关基因的mRNA和蛋白表达发育性变化规律[J]. 动物营养学报, 2018 , 30(10) : 4229 -4236 . DOI: 10.3969/j.issn.1006-267x.2018.10.049

Abstract

The experiment was conducted to research the developmental changes of mRNA and protein expression of adiponectin (ADIPOQ), adiponectin receptors (ADIPOR) and related gene in Shaziling pigs. The developmental changes on mRNA and protein expression of ADIPOQ, ADIPOR1, ADIPOR2, topoisomerase 2β (TOP2β) and TATA binding protein (TBP) of different tissues and organs were measured in 12 Shaziling pigs (1, 28 and 140 days of age, 4 head per age). The results showed that the mRNA expressions of ADIPOQ, ADIPOR1, ADIPOR2, TOP2β and TBP in different tissues and organs of pigs were tend to increase with the growth of pigs, the mRNA expressions of the above genes in most tissues and organs on 140 day of age were significantly higher than those on 1 day of age (P<0.05 or P<0.01). The protein expressions of ADIPOD, ADIPOR1 and ADIPOR2 in most tissue and organ showed a decreased trend. The results showed that the mRNA expressions of related gene and proteins of ADIPOQ are negatively correlation with the fat deposition of pigs, and the body can adjust according to its own needs.

Key words： adiponectin; adiponectin receptor; gene; developmental change; Shaziling pigs

参考文献

[1] ?EPICA S,MASOPUST M,KNOLL A,et al.Linkage and RH mapping of the porcine adiponectin gene on chromosome 13[J].Animal Genetics,2005,36(3):276-277.  

[2] DAI M H,XIA T,ZHANG G D,et al.Cloning,expression and chromosome localization of porcine adiponectin and adiponectin receptors genes[J].Domestic Animal Endocrinology,2006,30(2):117-125.  

[3] YAMAUCHI T,KAMON J,MINOKOSHI Y,et al.Adiponectin stimulates glucose utilization and fatty-acid oxidation by activating AMP-activated protein kinase[J].Nature Medicine,2002,8(11):1288-1295.  

[4] KADOWAKI T,YAMAUCH T,KUBOTA N,et al.Adiponectin and adiponectin receptors in obesity-linked insulin resistance[J].Novartis Foundation Symposium,2007,286:164-176.

[5] DING S T,LIU B H,KO Y H.Cloning and expression of porcine adiponectin and adiponectin receptor 1 and 2 genes in pigs[J].Journal of Animal Science,2004,82(11):3162-3174.  

[6] 宋虎威.沙子岭猪脂联素基因的克隆及其真核表达[D].硕士学位论文.长沙:湖南农业大学,2012.

[7] SIMMS D,CIZDZIEL P E,CHOMCZYNSKI P.TrizolTM:a new reagent for optimal single-step isolation of RNA[J].Focus,1993,15:532-535.

[8] RAMSAY T G,CAPERNA T J.Ontogeny of adipokine expression in neonatal pig adipose tissue[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,2009,152(1):72-78.  

[9] LI C J,ZHU F L,SUN H W,et al.Cloning of rabbit adiponectin and its relationship to age and high-cholesterol diet[J].Journal of Endocrinological Investigation,2008,31(9):755-759.  

[10] LEDOUX S,CAMPOS D B,LOPES F L,et al.Adiponectin induces periovulatory changes in ovarian follicular cells[J].Endocrinology,2006,147(11):5178-5186.  

[11] OCÓN-GROVE O M,KRZYSIK-WALKER S M,MADDINENI S R,et al.Adiponectin and its receptors are expressed in the chicken testis:influence of sexual maturation on testicular ADIPOR1 and ADIPOR2 mRNA abundance[J].Reproduction,2008,136(5):627-638.  

[12] PISCHON T,GIRMAN C J,RIFAI N,et al.Association between dietary factors and plasma adiponectin concentrations in men[J].The American Journal of Clinical Nutrition,2005,81(4):780-786.  

[13] DE ROSA A,MONACO M L,NIQRO E,et al.Tissue-specific downregulation of the adiponectin "system":possible implications for fat accumulation tendency in the pig[J].Domestic Animal Endocrinology,2013,44(3):131-138.  

[14] GAO R,SCHELLBNERG M J,HUANG S Y N,et al.Proteolytic degradation of topoisomerase Ⅱ (Top2) enables the processing of Top2·DNA and Top2·RNA covalent complexes by tyrosyl-DNA-phosphodiesterase 2(TDP2)[J].Journal of Biological Chemistry,2014,289(26):17960-17969.  
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2