动物营养学报 >

2019 , Vol. 31 >Issue 4: 1564 - 1573

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.04.013

猪与禽营养 Swine and poultry nutrition

卷曲乳杆菌对生长猪生长性能、粪便菌群和短链脂肪酸组成以及血清长链脂肪酸组成的影响

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  • 北京市农林科学院畜牧兽医研究所, 北京 100097
张董燕(1981-),女,河北邯郸人,博士,副研究员,主要从事动物营养研究。E-mail:zhdy203@126.com

收稿日期: 2018-09-19

  网络出版日期: 2019-04-16

基金资助

生猪产业技术体系北京市创新团队建设项目（BAIC02-2018）；北京市农林科学院科技创新能力建设专项（KJCX20161503）；北京市农林科学院青年科研基金（QNJJ201827）

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Effects of Lactobacillus crispatus on Growth Performance, Fecal Microbiota and Short Chain Fatty Acid Composition and Serum Long Chain Fatty Acid Composition of Growing Pigs

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  • Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China

Received date: 2018-09-19

  Online published: 2019-04-16

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摘要

本试验旨在研究猪源卷曲乳杆菌对生长猪生长性能、粪便菌群和短链脂肪酸组成以及血清长链脂肪酸组成的影响。选取体重（20.35±0.53）kg的二元杂交（长×大）生长猪120头，随机分成2组，每组4个重复，每个重复15头。对照组饲喂基础饲粮，试验组在基础饲粮中添加卷曲乳杆菌冻干制剂（卷曲乳杆菌添加量为5.5×109 CFU/kg），饲喂期为30 d。饲养试验结束当天采集新鲜粪便用于16S rRNA V3~V4区菌群和短链脂肪酸组成的测定，采集血清用于长链脂肪酸组成的测定。结果显示：1）与对照组相比，试验组生长猪平均日增重提高了7.32%（P<0.05），料重比降低了5.68%（P<0.05）；2）饲粮中添加卷曲乳杆菌能够提高生长猪粪便菌群物种丰富度，增加厚壁菌门及乳酸杆菌属和光岗菌属的比例；3）与对照组相比，试验组生长猪粪便中丁酸含量增加了6.20%（P<0.05），乙酸含量减少了9.88%（P<0.05）；4）试验组猪群血清中亚油酸和花生四烯酸含量分别比对照组提高了11.12%（P<0.05）和6.28%（P<0.05）。上述结果表明，在饲粮中添加5.5×109 CFU/kg卷曲乳杆菌能够影响生长猪的粪便菌群组成，增加粪便中丁酸的含量，并改善血清中不饱和脂肪酸的组成，从而对生长猪的肠道健康及生长起到促进作用。

关键词： 卷曲乳杆菌; 生长猪; 生长性能; 菌群组成; 短链脂肪酸; 长链脂肪酸

本文引用格式

张董燕, 季海峰, 刘辉, 王四新, 张伟, 王晶, 王雅民 . 卷曲乳杆菌对生长猪生长性能、粪便菌群和短链脂肪酸组成以及血清长链脂肪酸组成的影响[J]. 动物营养学报, 2019 , 31(4) : 1564 -1573 . DOI: 10.3969/j.issn.1006-267x.2019.04.013

Abstract

This experiment was conducted to study the effects of Lactobacillus crispatus on growth performance, fecal microbiota and short chain fatty acid composition and serum long chain fatty acid composition of growing pigs. A total of 120 two-way cross (Landrace×Yorkshire) growing pigs with an average body weight of (20.35±0.53) kg were randomly distributed into 2 groups with 4 replicates per group and 15 pigs per replicate. Piglets in control group were fed a basal diet, and those in trial group were fed the basal diet supplemented with Lactobacillus crispatus preparation (the Lactobacillus crispatus supplemental level was 5.5×109 CFU/kg). The experiment period was 30 d. Feces samples were collected and used for sequencing of the V3 to V4 hypervariable region of the 16S rRNA gene. Feces samples were also used to measure the short chain fatty acid composition and serum samples were analyzed for long chain fatty acid composition. The results showed as follows:1) compared with the control group, the average daily gain (ADG) was increased by 7.32% (P<0.05), and feed/gain (F/G) was decreased by 5.68% (P<0.05) in the trial group. 2) Dietary supplemented with Lactobacillus crispatus increased the microbiota richness, the Firmicutes at phylum, the Lactobacillus and Megasphaera at genus were also increased compared with the control group. 3) The content of butyric acid in feces was increased by 6.20% (P<0.05), and the content of acetic acid in feces was decreased by 9.88% (P<0.05) in the trial group compared with the control group. 4) The contents of serum C18:2n6c and C20:4n6 were increased by 11.12% (P<0.05) and 6.28% (P<0.05) in trial group, respectively. The results indicate that Lactobacillus crispatus has a beneficial effect on the intestinal microbiota composition, and increases the content of butyric acid in feces, improves serum long chain fatty acid composition, and then promotes the intestinal health and growth of growing pigs.

Key words： Lactobacillus crispatus; growing pigs; growth performance; microbiota composition; short chain fatty acids; long chain fatty acids

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