猪与禽营养 Swine and poultry nutrition

豆粕的鸭酶水解物总能与代谢能相关性研究

展开
  • 中国农业科学院北京畜牧兽医研究所, 动物营养学国家重点实验室, 北京 100193
魏杰(1992-),男,河南安阳人,硕士研究生,从事饲料营养价值评定研究。E-mail:weijiejawin@163.com

收稿日期: 2018-09-29

  网络出版日期: 2019-04-16

基金资助

现代水禽产业技术体系建设专项资金(CARS-42)

Correlation between Enzyme Hydrolysate Gross Energy and Metabolizable Energy of Soybean Meal for Ducks

Expand
  • State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2018-09-29

  Online published: 2019-04-16

摘要

本试验旨在研究不同粗蛋白质(CP)含量豆粕的鸭酶水解物总能(EHGE)与代谢能(ME)的相关性。采用豆皮和去皮豆粕配制成CP含量分别为36%、38%、40%、42%和44%的5种调制豆粕,再将其与玉米淀粉配制成CP含量均为20%的5种混合饲粮。采用仿生消化法测定饲料原料和混合饲粮的鸭EHGE,每种待测料5个重复,每个重复1根消化管;采用代谢试验测定5种混合饲粮和玉米淀粉的ME,每种混合饲粮选用10只成年雄性北京鸭进行代谢试验。结果显示:1)各种饲料原料和混合饲粮EHGE的变异系数(CV)均小于2.0%,表明仿生消化法测定EHGE具有良好的精确度。2)5种混合饲粮的EHGE实测值与其表观代谢能(AME)、真代谢能(TME)均呈显著线性正相关,回归方程分别为AME=1.028×EHGE-0.759(R2=0.934,P=0.007 3),TME=1.062×EHGE+0.221(R2=0.953,P=0.004 5)。3)5种调制豆粕的EHGE实测值分别为10.71、11.55、12.09、12.89、13.54 MJ/kg,AME分别为11.07、11.94、12.22、12.61、13.72 MJ/kg,TME分别为12.37、13.23、13.67、13.98、15.07 MJ/kg,EHGE实测值与AME、TME也均呈显著线性正相关,回归方程分别为AME=0.853×EHGE+1.941(R2=0.952,P=0.004 8),TME=0.877×EHGE+3.004(R2=0.960,P=0.003 5)。由此可见,调制豆粕和混合饲粮的鸭EHGE与ME均呈显著线性正相关,可以通过线性回归模型以其EHGE估测ME,有助于鸭饲粮有效能的快速测定。

本文引用格式

魏杰, 谢明, 张琪, 唐静, 侯水生 . 豆粕的鸭酶水解物总能与代谢能相关性研究[J]. 动物营养学报, 2019 , 31(4) : 1623 -1629 . DOI: 10.3969/j.issn.1006-267x.2019.04.019

Abstract

The purpose of this experiment was to study the correlation between enzyme hydrolysate gross energy (EHGE) and metabolizable energy (ME) for ducks based on soybean meal with different crude protein (CP) contents. Soybean hull and dehulled soybean meal were mixed into 5 kinds of modulation soybean meal with CP contents of 36%, 38%, 40%, 42% and 44%, respectively, and these 5 kinds of modulation soybean meal and corn starch were formulated into 5 kinds of mixed diets with 20% CP content. The EHGE of all feedstuffs and mixed diets were determined by simulated digestion method in vitro, and each sample was assigned with 5 replicates, and 1 digestion tube per replicate. The ME of 5 kinds of mixed diets and corn starch were determined by metabolism trial, and 10 adult male Pekin ducks were selected for the metabolism trial in each mixed diet. The results showed as follows:1) the coefficient of variation (CV) of EHGE of all feedstuffs and mixed diets was less than 2.0%, so there was good accuracy in EHGE determination by simulated digestion method in vitro. 2) There were significantly linear positive correlations between EHGE measured value and apparent metabolic energy (AME) and true metabolic energy (TME) of 5 kinds of mixed diets, and the regression equations were as follows:AME=1.019×EHGE-0.634 (R2=0.934, P=0.007 3), TME=1.062×EHGE+0.221 (R2=0.953, P=0.004 5). 3) The EHGE measured values of 5 kinds of modulation soybean meal were 10.71, 11.55, 12.09, 12.89 and 13.54 MJ/kg, respectively; AME were 11.07, 11.94, 12.22, 12.61 and 13.72 MJ/kg, respectively; and TME were 12.37, 13.23, 13.67, 13.98 and 15.07 MJ/kg, respectively. There were significantly linear positive correlations between EHGE measured value and AME, TME, and the regression equations were as follows:AME=0.853×EHGE+1.941 (R2=0.952, P=0.004 8), TME=0.877×EHGE+3.004 (R2=0.960, P=0.003 5). In conclusion, there is a significantly linear positive correlation between EHGE and ME of modulation soybean meal and mixed diet for ducks, so ME can be predicted by EHGE by establishing linear regression model, and in order to rapidly determine the effective energy of feeds for ducks.

Key words: ducks; soybean meal; EHGE; ME; correlation

参考文献

[1] 孙小恒,高玉鹏,齐广海,等.利用动物饲养试验评估优质豆粕代谢能的研究[J].西北农业学报,2008,17(2):43-47.

[2] 沈慧乐.豆粕是家禽理想的蛋白质[J].中国家禽,2005,27(18):32-35.

[3] YEGANI M,SWIFT M L,ZIJLSTRA R T,et al.Prediction of energetic value of wheat and triticale in broiler chicks:a chick bioassay and an in vitro digestibility technique[J].Animal Feed Science and Technology,2013,183(1/2):40-50.

[4] MOEHN S,ATAKORA J,BALL R O.Using net energy for diet formulation:potential for the Canadian pig industry[J].Advances in Pork Production,2005,16:119-127.

[5] KONG C,ADEOLA O.Evaluation of amino acid and energy utilization in feedstuff for swine and poultry diets[J].Asian-Australasian Journal of Animal Sciences,2014,27(7):917-925.  

[6] HUANG G,SAUER W C,HE J,et al.The nutritive value of hulled and hulless barley for growing pigs.1.Determination of energy and protein digestibility with the in vivo and in vitro method[J].Journal of Animal and Feed Sciences,2003,12(4):759-769.  

[7] 叶宏涛,刘国华.体外消化法在动物上的应用[J].饲料工业,2007,28(21):20-23.

[8] 张铁鹰,汪儆,卢庆萍,等.肉仔鸡体外消化模拟技术的研究[J].中国农业科学,2007,40(11):2600-2606.

[9] 赵峰.用酶法评定鸭饲料代谢能的方法学研究[D].博士学位论文.北京:中国农业科学院,2006:73-78.

[10] ZHAO F,ZHANG L,MI B M,et al.Using a computer-controlled simulated digestion system to predict the energetic value of corn for ducks[J].Poultry Science,2014,93(6):1410-1420.  

[11] 施学仕,聂光达.猪体外消化试验方法的研究[J].上海农业学报,1985(1):68-77.

[12] 张丽英.饲料分析及饲料质量检测技术[M].3版.北京:中国农业大学出版社,2007:48-183.

[13] 李辉,赵峰,计峰,等.仿生消化系统测定鸭饲料原料代谢能的重复性与精密度检验[J].动物营养学报,2010,22(6):1709-1716.

[14] 赵峰,李辉,张宏福.单胃动物仿生消化系统测定鸭饲料酶水解物能值可加性的研究[J].动物营养学报,2015,27(2):495-502.

[15] 张春雷.鸭饲料代谢能的生物学评定方法研究[D].硕士学位论文.杨凌:西北农林科技大学,2004:38-40.

[16] 张婵娟,王建萍,丁雪梅,等.不同来源豆粕对大恒肉鸡的能量和氨基酸营养价值评定[J].动物营养学报,2018,30(4):1320-1332.

[17] ADEOLA O,RAGLAND D,KING D.Feeding and excreta collection techniques in metabolizable energy assays for ducks[J].Poultry Science,1997,76(5):728-732.  

[18] 赵江涛,赵峰,张宏福,等.直接法与差量法测定豆粕鸭代谢能值的比较研究[J].动物营养学报,2008,20(3):242-248.

[19] 付趁,马慧慧,徐彬,等.体内法和体外法对玉米代谢能的评定效果比较[J].河南农业科学,2017,46(2):127-130,135.

[20] 郑卫宽.用仿生消化仪测定棉粕鸭真代谢能值的研究[D].硕士学位论文.北京:中国农业科学院,2009:35-36.

[21] 赵峰,张宏福,张子仪.基于模拟消化液和开发仿生消化系统评定家禽饲料代谢能值的研究进展[C]//2010中国畜牧兽医学会动物营养学分会第六次全国饲料营养学术研讨会论文集.杨凌:中国畜牧兽医学会,2010.

[22] 赵峰,李辉,米宝民,等.基于密闭容器的体外消化法与仿生消化系统估测玉米鸭代谢能值的比较研究[C]//第七届中国饲料营养学术研讨会论文集.郑州:中国畜牧兽医学会动物营养学分会,2014.

[23] 赵峰,赵江涛,张宏福.基于人工消化液与密闭消化器的酶法测定豆粕鸭代谢能值的研究[J].畜牧兽医学报,2011,42(7):946-954.
文章导航

/