动物营养学报 >

2019 , Vol. 31 >Issue 4: 1754 - 1764

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.04.033

饲料科学与技术 Feed science and technology

α-硫辛酸对热应激绵羊生长性能、抗氧化及免疫的影响

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  • 1. 河南农业大学牧医工程学院, 农业部动物生化与营养重点试验室, 郑州 450002;
    2. 河南农业大学现代实验技术与管理中心, 郑州 450002
张红瑞(1988-),女,河南信阳人,博士研究生,从事动物营养与饲料科学。E-mail:hongrui0207@163.com

收稿日期: 2018-09-25

  网络出版日期: 2019-04-16

基金资助

国家现代奶牛产业技术体系建设专项（CARS-36）；河南省现代农业产业技术体系专项基金（S2013-08-01）

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Effects of α-Lipoic Acid on Growth Performance, Antioxidant and Immunity of Sheep under Heat Stress

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  • 1. Key Laboratory of Animal Biochemistry and Nutrition of Ministry of Agriculture, College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China;
    2. Advanced Experimental Techniques and Managing Centre, Henan Agricultural University, Zhengzhou 450002, China

Received date: 2018-09-25

  Online published: 2019-04-16

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摘要

本试验旨在研究α-硫辛酸（α-LA）对热应激绵羊生长性能、抗氧化及免疫的影响，以期阐述其抗热应激的机理。选择4~5月龄、体重35~40 kg的绵羊15只，随机分为对照组（CTL）、低剂量组（LA-L，添加600 mg/kg α-LA）、高剂量组（LA-H，添加900 mg/kg α-LA），在热应激条件下（7~8月）饲喂40 d。结果表明：1）与对照组相比，α-LA添加组平均日采食量增加，低剂量组和高剂量组分别比对照组增加了7.3%和2.7%（P>0.05）；高剂量组血清中丙氨酸转氨酶（ALT）、门冬氨酸转氨酶（AST）活性及α-LA添加组血浆中丙二醛（MDA）、肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）、白细胞介素-8（IL-8）含量显著下降（P<0.05）；α-LA添加组血浆中三碘甲腺原氨酸（T3）、热休克蛋白70（HSP70）、免疫球蛋白G（IgG）含量及超氧化物歧化酶（SOD）活性显著升高（P<0.05）。2）与对照组相比，α-LA添加组肝脏中炎性相关因子IL-1β、IL-8、核因子-κB（NF-κB）mRNA表达量显著下降（P<0.05），抗炎因子干扰素-γ（IFN-γ）mRNA表达量显著升高（P<0.05）。综上，α-LA显著增强热应激绵羊的抗氧化能力，减少炎症相关因子的表达，增强抗炎因子的表达，提高机体免疫机能，且与α-LA剂量有关。

关键词： &alpha; -硫辛酸; 热应激; 生化指标; 免疫; 抗氧化; 炎性因子; 绵羊

本文引用格式

张红瑞, 高燕, 闫灵敏, 杨改青, 王林枫, 付彤, 廉红霞, 高腾云, 李明 . α-硫辛酸对热应激绵羊生长性能、抗氧化及免疫的影响[J]. 动物营养学报, 2019 , 31(4) : 1754 -1764 . DOI: 10.3969/j.issn.1006-267x.2019.04.033

Abstract

The aim of this study was to investigate the effects of α-lipoic acid (α-LA) on the growth performance, antioxidant and immunity of sheep under heat stress, in order to elucidate the mechanism of its anti-heat stress. Fifteen sheep aged 4 to 5 months and bodyweight between 35 to 40 kg were randomly selected, and equally divided into three groups:control group (CTL), low-dose group (LA-L, supplemented with 600 mg/kg α-LA) and high-dose group (LA-H, supplemented with 900 mg/kg α-LA), and animals fed under heat stress (7 to 8 months) for 40 d. The results showed as follows:1) compared with control group, average daily feed intake in α-LA group increased, and that in low-dose group and high-dose group increased by 7.3% and 2.7% (P>0.05); serum alanine aminotransferase, aspartate aminotransferase activities in high-dose group, malonaldehyde, tumor necrosis factor-α, interleukin-1β, interleukin-8 in α-LA group significantly decreased (P<0.05); the plasma contents of triiodothyronine, heat shock protein 70, immunoglobulin G, and superoxide dismutase activity in α-LA group significantly increased (P<0.05). 2) Compared with control group, the mRNA expression levels of inflammatory cytokines interleukin-1β, interleukin-8, nuclear factor-κB significantly decreased (P<0.05), and the mRNA expression levels of anti-inflammatory cytokine interferon-γ significantly increased (P<0.05). To sum up, α-LA significantly enhances the antioxidant capacity of sheep under heat stress, decreases the expression of inflammatory factors, and improves the expression of anti-inflammatory factors and immune function; the effect is related to the dosage of α-LA.

Key words： α-lipoic acid; heat stress; biochemical indexes; immune; anti-oxidation; inflammatory factors; sheep

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