动物营养学报 >

2019 , Vol. 31 >Issue 4: 1789 - 1796

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.04.037

饲料科学与技术 Feed science and technology

凹土纳米氧化锌对断奶仔猪血清、肝脏和肠道黏膜抗氧化指标的影响

展开
  • 扬州大学动物科学与技术学院, 扬州 225009
毛俊舟(1993-),男,安徽六安人,硕士研究生,从事单胃动物营养研究。E-mail:781446817@qq.com

收稿日期: 2018-09-12

  网络出版日期: 2019-04-16

基金资助

江苏省基础研究计划（自然科学基金）-青年基金项目（BK20170490）；扬州市产学研合作专项（市校合作）（YZ2016257）；扬州大学镇江高新技术研究院开放课题

收起

Effects of Attapulgite Nano Zinc Oxide on Antioxidant Indexes in Serum, Liver and Intestinal Mucosa of Weaned Piglets

Expand
  • College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2018-09-12

  Online published: 2019-04-16

Fold

摘要

本试验旨在研究凹土纳米氧化锌对断奶仔猪血清、肝脏和肠道黏膜抗氧化指标的影响。选取210头21日龄体重（6.30±0.51）kg的健康杜×长×大断奶仔猪，随机分为7个组，每组6个重复，每个重复5头猪。各组分别为对照组（CON组，基础饲粮）、抗生素组（ANT组，基础饲粮+抗生素）、氧化锌组（ZO组，基础饲粮+3 000 mg/kg氧化锌）、纳米氧化锌组（NZO组，基础饲粮+800 mg/kg纳米氧化锌）、低剂量凹土纳米氧化锌组（LANZ组，基础饲粮+700 mg/kg凹土纳米氧化锌）、中剂量凹土纳米氧化锌组（MANZ组，基础饲粮+1 000 mg/kg凹土纳米氧化锌）和高剂量凹土纳米氧化锌组（HANZ组，基础饲粮+1 300 mg/kg凹土纳米氧化锌）。预试期3 d，正试期9 d。结果表明：1）ZO和LANZ组的血清过氧化氢酶（CAT）活性显著高于CON组（P<0.05）；ANT、ZO、LANZ、MANZ和HANZ组的血清总抗氧化能力（T-AOC）显著高于CON组（P<0.05），且ANT组的血清T-AOC显著高于其他各组（P<0.05）。2）LANZ组的肝脏CAT活性显著高于其他各组（P<0.05）。3）ZO、NZO、LANZ、MANZ和HANZ组的空肠黏膜超氧化物歧化酶（SOD）活性显著高于ANT组（P<0.05）。ZO、LANZ组的空肠黏膜CAT活性显著高于CON和ANT组（P<0.05）。ANT、ZO、NZO、LANZ和HANZ组的空肠黏膜T-AOC显著高于CON和MANZ组（P<0.05）。4）HANZ组的回肠黏膜CAT活性显著低于CON组（P<0.05），ZO、NZO组的回肠黏膜谷胱甘肽过氧化物酶（GSH-Px）活性显著低于CON、ANT和LANZ组（P<0.05）。由此可见，断奶仔猪饲粮中添加适量凹土纳米氧化锌能够提高血清、肝脏和肠道中抗氧化酶的活性，增强仔猪的抗氧化性能。本试验中，饲粮中凹土纳米氧化锌适宜添加量为700 mg/kg。

关键词： 凹土纳米氧化锌; 血清; 肝脏; 肠道黏膜; 抗氧化

本文引用格式

毛俊舟, 董丽, 彭程, 张永胜, 喻礼怀 . 凹土纳米氧化锌对断奶仔猪血清、肝脏和肠道黏膜抗氧化指标的影响[J]. 动物营养学报, 2019 , 31(4) : 1789 -1796 . DOI: 10.3969/j.issn.1006-267x.2019.04.037

Abstract

This experiment was conducted to explore the effects of attapulgite nano zinc oxide on antioxidant indexes in serum, liver and intestinal mucosa of weaned piglets. A total of 210 healthy 21-day-old piglets with a similar body weight of (6.30±0.51) kg were randomly allocated into 7 groups with 6 replicates per group and 5 piglets per replicate. The 7 groups were control group (CON group, basal diet), antibiotic group (ANT group, basal diet+antibiotic), zinc oxide group (ZO group, basal diet+3 000 mg/kg zinc oxide), nano zinc oxide group (NZO group, basal diet+800 mg/kg nano zinc oxide), low dose attapulgite nano zinc oxide group (LANZ group, basal diet+700 mg/kg attapulgite nano zinc oxide), middle dose attapulgite nano zinc oxide group (MANZ group, basal diet+1 000 mg/kg attapulgite nano zinc oxide) and high dose attapulgite nano zinc oxide group (HANZ group, basal diet+1 300 mg/kg attapulgite nano zinc oxide). The pre-experimental period lasted for 3 days, and the experimental period lasted for 9 days. The results showed as follows:1) the serum catalase (CAT) activity of ZO and LANZ groups was significantly higher than that of CON group (P<0.05); the serum total antioxidant capacity (T-AOC) of ANT, ZO, LANZ, MANZ and HANZ groups was significantly higher than that of CON group (P<0.05), and the serum T-AOC of ANT group was significantly higher than that of other groups (P<0.05). 2) The liver CAT activity of LANZ group was significantly higher than that of other groups (P<0.05). 3) The jejunum mucosa superoxide dismutase (SOD) activity of ZO, NZO, LANZ, MANZ and HANZ groups was significantly higher than that of ANT group (P<0.05). The jejunum mucosa CAT activity of ZO and LANZ groups was significantly higher than that of CON and ANT groups (P<0.05). The jejunum mucosa T-AOC of ANT, ZO, NZO, LANZ and HANZ groups was significantly higher than that of CON and MANZ groups (P<0.05). 4) The ileal mucosa CAT activity of HANZ group was significantly lower than that of CON group (P<0.05), the ileal mucosa glutathione peroxidase (GSH-Px) activity of ZO and NZO groups was significantly lower than that of CON, ANT and LANZ groups (P<0.05). The results indicat that the appropriate supplementation of attapulgite nano zinc oxide can improve the activity of antioxidant enzymes in serum, liver and intestine, and enhance the antioxidant performance of piglets. The suitable supplementation of attapulgite nano zinc oxide is 700 mg/kg in this experiment.

Key words： attapulgite nano zinc oxide; serum; liver; intestinal mucosa; antioxidant

参考文献

[1] HU C H,SONG J,YOU Z T,et al.Zinc oxide-montmorillonite hybrid influences diarrhea,intestinal mucosal integrity,and digestive enzyme activity in weaned pigs[J].Biological Trace Element Research,2012,149(2):190-196.  

[2] 陈凤鸣,陈佳亿,彭伟,等.氧化应激对猪肠道损伤机制的研究进展[J].动物营养学报,2018,30(8):2887-2893.

[3] HOFER U.Microbiome:effects of in-feed antibiotics on pig microbiota[J].Nature Reviews Microbiology,2014,12(4):234.

[4] BARTON M D.Antibiotic use in animal feed and its impact on human healt[J].Nutrition Research Reviews,2000,13(2):279-299.  

[5] SAHOO A,SWAIN R K,MISHRA S K.Effect of inorganic,organic and nano zinc supplemented diets on bioavailability and immunity status of broilers[J].International Journal of Advanced Research,2014,2(11):828-837.

[6] 毛俊舟,董丽,王淑楠,等.凹土纳米氧化锌对断奶仔猪生长性能、器官指数及血液生化指标的影响[J].动物营养学报,2018,30(4):1471-1480.

[7] 方洛云,邹晓庭,蒋林树,等.不同锌源对断奶仔猪免疫和抗氧化作用的影响[J].中国兽医学报,2005,25(2):201-203.

[8] SWAIN P S,RAO S B N,RAJENDRAN D,et al.Nano zinc,an alternative to conventional zinc as animal feed supplement:a review[J].Animal Nutrition,2016,2(3):134-141.  

[9] WANG B,FENG W Y,WANG M,et al.Acute toxicological impact of nano-and submicro-scaled zinc oxide powder on healthy adult mice[J].Journal of Nanoparticle Research,2008,10(2):263-276.  

[10] CHEN H,WANG A Q.Kinetic and isothermal studies of lead ion adsorption onto palygorskite clay[J].Journal of Colloid and Interface Science,2007,307(2):309-316.  

[11] WANG W B,TIAN G Y,ZHANG Z F,et al.A simple hydrothermal approach to modify palygorskite for high-efficient adsorption of methylene blue and Cu(Ⅱ) ions[J].Chemical Engineering Journal,2015,265:228-238.

[12] ZHOU S Y,XUE A L,ZHANG Y,et al.Novel polyamidoamine dendrimer-functionalized palygorskite adsorbents with high adsorption capacity for Pb2+ and reactive dyes[J].Applied Clay Science,2015,107:220-229.

[13] EU Commission.Commission regulation (EU) No. 575/2011 of 16 June 2011 on the catalogue of feed materials[J].Official Journal of the European Union,2011,159:25-65.

[14] MASIMANGO N,REMACLE J,RAMAUT J L.The role of adsorption in the elimination of aflatoxin B1 from contaminated media[J].European Journal of Applied Microbiology and Biotechnology,1978,6(1):101-105.  

[15] 朱振海,郑茂松,孙新友,等.凹凸棒石粘土净化含强致癌物质黄曲霉毒素B1油脂工艺技术研究[J].非金属矿,1998,21(6):15-17.

[16] TANG C H,WANG X Q,ZHANG J M.Effects of supplemental palygorskite instead of zinc oxide on growth performance,apparent nutrient digestibility and fecal zinc excretion in weaned piglets[J].Animal Science Journal,2014,85(4):435-439.  

[17] LV Y F,TANG C H,WANG X Q,et al.Effects of dietary supplementation with palygorskite on nutrient utilization in weaned piglets[J].Livestock Science,2015,174:82-86.

[18] TOMITA T,SADAKATA H,TAMURA M,et al.Indomethacin-induced generation of reactive oxygen species leads to epithelial cell injury before the formation of intestinal lesions in mice[J]. Journal of Physiology and Pharmacology,2014,65(3):435-440.

[19] GIANNENAS I,DOUKAS D,KARAMOUTSIOS A,et al.Effects of Enterococcus faecium,mannan oligosaccharide,benzoic acid and their mixture on growth performance,intestinal microbiota,intestinal morphology and blood lymphocyte subpopulations of fattening pigs[J].Animal Feed Science and Technology,2016,220:159-167.

[20] LAN R X,KIM Y M,YUN H M,et al.Effects of Enterococcus faecium on growth performance,blood parameters,relative organ weight,breast muscle meat quality,excreta microbial shedding and noxious gas emission in broilers[J].Journal of Animal Science,2017,95(Suppl.2):125.

[21] 曹蓉,谢堃,侯德兴,等.厚朴总酚抗氧化作用及其机制研究进展[J].中国畜牧兽医,2016,43(9):2395-2400.

[22] GU X H,HAO Y,WANG X L.Overexpression of heat shock protein 70 and its relationship to intestine under acute heat stress in broilers:2.Intestinal oxidative stress[J].Poultry Science,2012,91(4):790-799.  

[23] REYES-BECERRIL M,TOVAR-RAMÍREZ D,ASCENCIO-VALLE F,et al.Effects of dietary live yeast Debaryomyces hansenii on the immune and antioxidant system in juvenile leopard grouper Mycteroperca rosacea exposed to stress[J].Aquaculture,2008,280(1/2/3/4):39-44.

[24] 冯梦.纳米氧化锌对小鼠十二指肠和空肠上皮细胞生长和抗氧化功能的影响[D].硕士学位论文.雅安:四川农业大学,2009.

[25] 李治学.新型氧化锌对断奶仔猪生产性能与肠道功能的影响及其作用机制研究[D].博士学位论文.杭州:浙江大学,2015.

[26] 潘建秋,蒲朝煜.氧自由基与肝细胞损伤[J].贵州医药,2000,24(12):756-758.

[27] SHAHEEN A A,EL-FATTAH A A A.Effect of dietary zinc on lipid peroxidation,glutathione,protein thiols levels and superoxide dismutase activity in rat tissues[J].The International Journal of Biochemistry & Cell Biology,1995,27(1):89-95.  

[28] BATTIN E E,BRUMAGHIM J L.Antioxidant activity of sulfur and selenium:a review of reactive oxygen species scavenging,glutathione peroxidase,and metal-binding antioxidant mechanisms[J].Cell Biochemistry and Biophysics,2009,55(1):1-23.  

[29] SERRA V,VON ZGLINICKI T,LORENZ M,et al.Extracellular superoxide dismutase is a major antioxidant in human fibroblasts and slows telomere shortening[J].Journal of Biological Chemistry,2003,278(9):6824-6830.  

[30] HU C H,XIAO K,SONG J,et al.Effects of zinc oxide supported on zeolite on growth performance,intestinal microflora and permeability,and cytokines expression of weaned pigs[J].Animal Feed Science and Technology,2013,181(1/2/3/4):65-71.
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2