动物营养学报 >

2019 , Vol. 31 >Issue 4: 1887 - 1896

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.04.048

研究简报 Short Communications

饲粮添加蒙脱石和枯草芽孢杆菌对产蛋鸡盲肠菌群和肠道通透性的影响

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  • 湖南农业大学动物科学技术学院, 湖南畜禽安全生产协同创新中心, 湖南家禽安全生产工程技术研究中心, 长沙 410128
曲湘勇(1962-),男,湖南长沙人,教授,博士,主要研究方向为家禽生产科学。E-mail:quxy99@126.com

收稿日期: 2018-09-30

  网络出版日期: 2019-04-16

基金资助

湖南畜禽安全生产协同创新中心专项资金（CICAPS）；湖南农业大学产学研合作项目（13098）

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Effects of Dietary Montmorillonite and Bacillus subtilis on Cecal Microflora and Intestinal Permeability of Laying Hens

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  • Hunan Co-Innovation Center of Animal Production Safety, Hunan Engineering Research Center of Poultry Production Safety, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China

Received date: 2018-09-30

  Online published: 2019-04-16

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摘要

本试验旨在研究饲粮添加蒙脱石（MMT）和枯草芽孢杆菌（BS）对产蛋鸡盲肠菌群和肠道通透性的影响。采用2×2双因子随机设计，选择360只29周龄健康的罗曼粉壳蛋鸡，随机分为4组（每组6个重复，每个重复15只），分别饲喂基础饲粮（对照组）、基础饲粮+0.5 g/kg MMT（MMT组）、基础饲粮+5×108 CFU/kg BS（BS组）、基础饲粮+0.5 g/kg MMT+5×108 CFU/kg BS（MMT+BS组）。预试期7 d，正试期70 d。结果表明：1）BS组测序获得的有效序列显著多于其他各组（P<0.05），试验组测序获得的优质序列较对照组均有一定增加（P>0.05）。2）在门水平上，与对照组相比，MMT组梭杆菌门相对含量有降低的趋势（P=0.072），MMT+BS组迷踪菌门相对含量有降低的趋势（P=0.053）。在属水平上，与对照组相比，MMT组梭杆菌属相对含量有降低的趋势（P=0.072），BS组乳杆菌属相对含量显著提高（P<0.05），BS组和MMT+BS组巨单胞菌属相对含量显著提高（P<0.05），MMT+BS组梭菌属相对含量显著降低（P<0.05）。3）与对照组相比，饲粮添加BS显著降低了产蛋鸡血浆二胺氧化酶活性和内毒素含量（P<0.05），有降低血浆D-乳酸含量的趋势（P=0.051）。可见，MMT、BS及其联用均在一定程度上影响了产蛋鸡盲肠菌群的物种丰度，添加BS降低了肠道通透性。

关键词： 蒙脱石; 枯草芽孢杆菌; 肠道菌群; 肠道通透性; 产蛋鸡

本文引用格式

曲湘勇, 陈继发, 匡佑华, 康克浪, 唐圣果, 贺长青 . 饲粮添加蒙脱石和枯草芽孢杆菌对产蛋鸡盲肠菌群和肠道通透性的影响[J]. 动物营养学报, 2019 , 31(4) : 1887 -1896 . DOI: 10.3969/j.issn.1006-267x.2019.04.048

Abstract

This study was conducted to evaluate the effects of dietary montmorillonite (MMT) and Bacillus subtilis (BS) on cecal microflora and intestinal permeability of laying hens. The trial used a 2×2 factorial random design. A total of 360 healthy 29-week-old Lohmann pink hens were randomly arranged into 4 groups with 6 replicates per group and 15 hens in each replicate. The hens in 4 groups were fed a basal diet (control group), the basal diet+0.5 g/kg MMT (MMT group), the basal diet+5×108 CFU/kg BS (BS group), and the basal diet+0.5 g/kg MMT+5×108 CFU/kg BS (MMT+BS group), respectively. The adjustment period lasted for 7 days, and the experimental period lasted for 70 days. The results showed as follows:1) the quantities of effective sequences observed in the BS group were more than those in the control group, MMT group and MMT+BS group (P<0.05); the quantities of good sequences observed in experimental groups were more than those in the control group (P>0.05). 2) At the phylum level, the relative content of Fusobacteria in the MMT group tended to be lower than that in the control group (P=0.072), and the relative content of Elusimicrobia in the MMT+BS group tended to be lower than that in the control group (P=0.053). At the genus level, the relative content of Fusobacterium in the MMT group tended to be lower than that in the control group (P=0.072). Compared with the control group, the relative content of Lactobacillus in the BS group was significantly increased (P<0.05), the relative content of Megamonas in BS and MMT+BS groups was significantly increased (P<0.05), and the relative content of Clostridium in the MMT+BS group was significantly decreased (P<0.05). 3) Compared with control group, dietary BS significantly decreased the activity of diamine oxidase and endotoxin content in plasma (P<0.05), and tended to reduce plasma content of D-lactic acid (P=0.051). In conclusion, the species abundance of cecal microbiota in laying hens can be partially influenced by single or combined addition of MMT and BS, and dietary supplementation with BS decreases intestinal permeability of laying hens.

Key words： montmorillonite; Bacillus subtilis; intestinal microflora; gut permeability; laying hens

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