反刍与草食动物营养 Ruminant and herbivore nutrition

限制或过量添加脂溶性维生素对育肥阶段杜寒杂交肉羊屠宰性能、内脏器官发育和肉品质的影响

展开
  • 1. 塔里木大学动物科学学院, 阿拉尔 843300;
    2. 中国农业科学院饲料研究所, 农业部饲料生物技术重点实验室, 北京 100081;
    3. 中国农业科学院农产品加工研究所, 北京 100193;
    4. 巴彦淖尔市农牧业科学研究院, 巴彦淖尔 015000;
    5. 内蒙古富川饲料科技股份有限公司, 巴彦淖尔 015000
周丽雪(1993-),女,新疆乌鲁木齐人,硕士研究生,从事反刍动物生理营养研究。E-mail:browneyes_daisy@sina.com

收稿日期: 2018-11-16

  网络出版日期: 2019-05-15

基金资助

现代农业产业技术体系建设专项资金资助(CARS-38);内蒙古自治区科技重大专项

Effects of Restriction and Excess Amount of Fat-Soluble Vitamins on Slaughter Performance, Internal Organ Development and Meat Quality of Dorper×Thin-Tailed Han Crossbred Lambs during Fattening Stage

Expand
  • 1. College of Animal Science, Tarim University, Alar 843300, China;
    2. Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    3. Institute of Agro-Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    4. Scientific Research Institute of Agricultural and Animal Husbandry in Bayanur, Bayanur 015000, China;
    5. Inner Mongolia Fuchuan Feed Science and Technology Ltd., Co., Bayanur 015000, China

Received date: 2018-11-16

  Online published: 2019-05-15

摘要

本试验通过研究饲粮中限制或过量添加脂溶性维生素(维生素A、维生素D、维生素E)对育肥阶段杜寒杂交肉羊屠宰性能、内脏器官发育和肉品质的影响,旨在评估NRC(2007)提出的脂溶性维生素推荐量是否适用于我国肉羊养殖。采用单因素试验设计,选择120只90日龄断奶后体重为(26.00±0.11) kg的杜寒杂交肉用绵羊,根据体重随机分为5组,每组12个重复,每个重复2只(公母各占1/2)。对照组(PC组)饲喂基础饲粮,按需要量提供维生素A、维生素D和维生素E复合制剂;试验组分别在PC组基础上扣除30%的维生素A(PC-VA组)、维生素D(PC-VD组)和维生素E(PC-VE组);另设过量添加组,其3种脂溶性维生素添加量均为对照组的7.5倍(PC-7.5组)。试验期100 d。结果表明:1)PV-7.5组的眼肌面积有高于PC组的趋势(P=0.06),各组肉羊的宰前活重(LWBS)、胴体重和屠宰率均差异不显著(P>0.05)。各组肉羊内脏器官重量及其占LWBS的比例均没有显著差异(P>0.05)。各组肉羊各胃室重量及其占复胃总重的比例均没有显著差异(P>0.05)。2)PC-7.5组的肉色亮度值显著低于除PC组外的其他各组(P<0.05);PC-VA组和PC-VE组的背最长肌C10:0含量有低于PC组的趋势(P=0.07),PC-VA组和PC-VE组的背最长肌C20:0含量有低于PC-7.5组的趋势(P=0.09);PC组的背最长肌半胱氨酸(Cys)含量显著低于其他各组(P<0.05),且PC组的背最长肌丝氨酸(Ser)含量显著低于PC-VA组和PC-7.5组(P<0.05)。结果提示,扣除30%或摄入过量的脂溶性维生素对杜寒杂交肉羊的屠宰性能和肌肉脂肪酸含量均无显著影响,但影响了肌肉氨基酸含量,从而对肉品质产生影响。

本文引用格式

周丽雪, 王振宇, 马涛, 崔凯, 杨东, 潘腾, 王文义, 王韵斐, 陈怀森, 刁其玉 . 限制或过量添加脂溶性维生素对育肥阶段杜寒杂交肉羊屠宰性能、内脏器官发育和肉品质的影响[J]. 动物营养学报, 2019 , 31(5) : 2176 -2186 . DOI: 10.3969/j.issn.1006-267x.2019.05.025

Abstract

The purpose of this experiment was to investigate the effects of restriction and excess amount of fat-soluble vitamins (vitamin A, vitamin D and vitamin E) on slaughter performance, internal organ development and meat quality of Dorper×thin-tailed Han crossbred lambs during fattening stage, with an aim to evaluate if the recommendation of fat-soluble vitamins provided by NRC (2007) is suitable for the sheep production in China. A total of 120 Dorper×thin-tailed Han crossbred sheep (half male and half female) with average body weight of (26.00±0.11) kg after weaning at 90 days of age were used in the current study according to a single factor experimental design. All sheep were randomly divided into 5 groups according to their body weight with 12 replicates per group and 2 sheep per replicate (half male and half female). The control group (PC group) fed a basal diet which provided vitamin A, vitamin D and vitamin E compounds as required; 30% of vitamin A (PC-VA group), vitamin D (PC-VD group) and vitamin E (PC-VE group) was deducted from PC group in the experimental groups, respectively; a high-dose group (PC-7.5 group) was set up which all fat-soluble vitamins were 7.5 times higher than that of the control group. The experiment lasted for 100 days. The results showed as follows: 1) the eye muscle area of PV-7.5 group was tended to higher than that of PC group (P=0.06), there were no significant differences in live weight before slaughter (LWBS), carcass weight and dressing percentage of sheep among all groups (P>0.05). There were no significant differences in internal organ weights and their percentage of LWBS of sheep among all groups (P>0.05). There were no significant differences in each stomach weights and their percentage of total compound stomach weight of sheep among all groups (P>0.05). 2) The meat color brightness value of PC-7.5 group was significantly lower than that of the other groups except the PC group (P<0.05); the Longissimus dorsi C10:0 content of PC-VA group and PC-VE group was tended to lower than that of PC group (P=0.07), the Longissimus dorsi C20:0 content of PC-VA group and PC-VE group was tended to lower than that of PC-7.5 group (P=0.09); the Longissimus dorsi cystine content of PC group was significantly lower than that of other groups (P<0.05), and the Longissimus dorsi serine content of PC group was significantly lower than that of PC-VA group and PC-7.5 group (P<0.05). The results suggeste that deduction of 30% or excess amount of fat-soluble vitamins intake have no significant impact on slaughter performance and muscle fatty acid content of Dorper×thin-tailed Han crossbred sheep, however, it affects the muscle amino acid content, which have potential impacts on meat quality.

参考文献

[1] 冯仰廉.反刍动物营养学[M].北京:科学出版社,2004.

[2] BENDICH A,GABRIEL E,MACHLIN L J.Dietary vitamin E requirement for optimum immune responses in the rat[J].The Journal of Nutrition,1986,116(4):675-681.  

[3] MACHLIN L J.Handbook of vitamins[M].2nd ed.New York:Marcel Dekker,Inc.,1991.

[4] SALVATORI G,PANTALEO L,DI CESARE C,et al.Fatty acid composition and cholesterol content of muscles as related to genotype and vitamin E treatment in crossbred lambs[J].Meat Science,2004,67(1):45-55.  

[5] TURNER K E,MCCLURE K E,WEISS W P,et al.Alpha-tocopherol concentrations and case life of lamb muscle as influenced by concentrate or pasture finishing[J].Journal of Animal Science,2002,80(10):2513-2521.

[6] MACIT M,AKSAKAL V,EMSEN E,et al.Effects of vitamin E supplementation on fattening performance,non-carcass components and retail cut percentages,and meat quality traits of Awassi lambs[J].Meat Science,2003,64(1):1-6.  

[7] ALVAREZ I,DE LA FUENTE J,DÍAZ M T,et al.Estimation of α-tocopherol concentration necessary to optimise lamb meat quality stability during storage in high-oxygen modified atmosphere using broken-line regression analysis[J].Animal,2008,2(9):1405-1411.  

[8] BHATT R S,SAHOO A,KARIM S A,et al.Effects of calcium soap of rice bran oil fatty acids supplementation alone and with DL-α-tocopherol acetate in lamb diets on performance,digestibility,ruminal parameters and meat quality[J].Journal of Animal Physiology and Animal Nutrition,2016,100(3):578-589.  

[9] OBROCHTA K M,KROIS C R,CAMPOS B,et al.Insulin regulates retinol dehydrogenase expression and all-trans-retinoic acid biosynthesis through FoxO1[J].Journal of Biological Chemistry,2015,290(11):7259-7268.  

[10] MANOLESCU D C,SIMA A,BHAT P V.All-trans retinoic acid lowers serum retinol-binding protein 4 concentrations and increases insulin sensitivity in diabetic mice[J].The Journal of Nutrition,2010,140(2):311-316.  

[11] REDONNET A,FERRAND C,BAIRRAS C,et al.Synergic effect of vitamin A and high-fat diet in adipose tissue development and nuclear receptor expression in young rats[J].British Journal of Nutrition,2008,100(4):722-730.  

[12] NETO O R M,CHIZZOTTI M L,RAMOS E M,et al.Fatty acid profile and meat quality of young bulls fed ground soybean or ground cottonseed and vitamin E[J].Animal,2015,9(2):362-372.  

[13] ZHOU G H,YANG A,TUME R K.A relationship between bovine fat colour and fatty acid composition[J].Meat Science,1993,35(2):205-212.  

[14] KRUK Z A,MALAU-ADULI A E O,PITCHFORD WS,et al.Genetics of fat colour in cattle[C]//Proceedings of the 6th World Congress on Genetics Applied to Livestock.Armidale,NSW,Australia,1998,23:121-124.

[15] DONOGHUE S,KRONFELD D S,SKLAN D.Retinol homeostasis in lambs given low and high intakes of vitamin A[J].British Journal of Nutrition,1983,50(2):235-248.  

[16] DONOGHUE S,DONAWICK W J,KRONFELD D S.Transfer of vitamin a from intestine to plasma in lambs fed low and high intakes of vitamin A[J].The British Journal of Nutrition,1983,113(11):2197-2204.  

[17] NRC.Nutrient requirements of sheep[S].6th ed.Washington D.C.:National Academy Press,1985.

[18] HENSEL D R,DIRECTOR C.Agricultural research center[Z].Hastings:Florida,1981.

[19] DE RANCOURT M,FOIS N,LAVÍN M P,et al.Mediterranean sheep and goats production:an uncertain future[J].Small Ruminant Research,2006,62(3):167-179.  

[20] KOBAYASHI S,TAKASAKI K.Effects of DL-α-tocopherol on body weight gains and meat qualities of holstein steers[J].Nihon Chikusan Gakkaiho,1985,56(10):802-806.  

[21] ARNOLD R N,SCHELLER K K,ARP S C,et al.Effect of long-or short-term feeding of α-tocopheryl acetate to Holstein and crossbred beef steers on performance,carcasscharacteristics,and beef color stability[J].Journal of Animal Science,1992,70(10):3055-3065.  

[22] KRUKA Z A,BOTTEMA C D K,DAVIS J J,et al.Effects of vitamin A on growth performance and carcass quality in steers[J].Livestock Science,2008,119(1/2/3):12-21.

[23] BIRCH K S,THOMAS J D,ROSS T T.Growth and carcass characteristics of newly received feeder lambs treated with probiotics and vitamin E[J].Sheep and Goat Research Journal,1994,10:201-206.

[24] CASSENSRG,FAUSTMAN C,JIMENEZ-COLMENEROF.Modern developments in research on color of meat[M]//KROL B,VAN ROON P,HOUBEN J.Trends in modern meat technology.Wageningen:The Netherlands,1988.

[25] MCKENNA D R,MIES P D,BAIRD B E,et al.Biochemical and physical factors affecting discoloration characteristics of 19 bovine muscles[J].Meat Science,2005,70(4):665-682.  

[26] LIU Q,LANARI M C,SCHAEFER D M.A review of dietary vitamin e supplementation for improvement of beef quality[J].Journal of Animal Science,1995,73(10):3131-3140.  

[27] D'ALESSANDRO A G,MAIORANO G,KOWALISZYNB,et al.How the nutritional value and consumer acceptability of suckling lambs meat is affected by the maternal feeding system[J].Small Ruminant Research,2012,106(2/3):83-91.

[28] 李晓亚,唐德富,李发弟,等.反刍动物肌肉脂肪酸对肉品质的影响及其调控因素[J].动物营养学报,2016,28(12):3749-3756.

[29] CABRERA M C,SAADOUN A.An overview of the nutritional value of beef and lamb meat from South America[J].Meat Science,2014,98(3):435-444.  

[30] ORIANI G,MAIORANO G,FILETTI F,et al.Effect of age on fatty acid composition of Italian Merino suckling lambs[J].Meat Science,2005,71(3):557-562.  

[31] ELMORE J E,MOTTRAM D S.The role of lipid in the flavour of cooked beef[J].Developments in Food Science,2006,43:375-378.

[32] 双金,敖力格日玛,敖长金.苏尼特羊体脂脂肪酸组成的研究[J].畜牧兽医学报,2015,46(8):1363-1374.

[33] ÁLVAREZ I,DE LA FUENTE J,CAÑEQUE V,et al.Changes in the fatty acid composition of M.longissimusdorsi of lamb during storage in a high-oxygen modified atmosphere at different levels of dietary vitamin E supplementation[J].Journal of Agricultural and Food Chemistry,2009,57(1):140-146.  

[34] BELLÉS M,LEAL L N,DÍAZ V,et al.Effect of dietary vitamin E on physicochemical and fatty acid stability of fresh and thawed lamb[J].Food Chemistry,2018,239:1-8.

[35] ARNETT A M,DIKEMAN M E,SPAETH C W,et al.Effects of vitamin A supplementation in young lambs on performance,serumlipid,andlongissimus muscle lipid composition[J].Journal of Animal Science,2007,85(11):3062-3071.  

[36] LIND V,BERG J,EILERTSEN S M,et al.Effect of gender on meat quality in lamb from extensive and intensive grazing systems when slaughtered at the end of the growing season[J].Meat Science,2011,88(2):305-310.  

[37] WHEELER K B,LATSHAW J D.Sulfur amino acid requirements and interactions in broilers during two growth periods[J].Poultry Science,1981,60(1):228-236.  

[38] MURDOCH R N.Metabolic fate of parenterally administered sulphur-containing amino acids in sheep and effects on growth and composition of wool[J].Australian Journal of Biological Sciences,1970,23(5):1077-1088.

[39] KATO N,MOCHIZUKI S,KAWAI K,et al.Effect of dietary level of sulfur-containing amino acids on liver drug-metabolizing enzymes,serum cholesterol and urinary ascorbic acid in rats fed PCB[J].The Journal of Nutrition,1982,112(5):848-854.  

[40] KATAYAMA T,MOMOTA Y,WATANABE Y,et al.Elevated concentrations of α-tocopherol,ascorbicacid,and serum lipids in rats fed polychlorinated biphenyls,chlorobutanol,or phenobarbital[J].The Journal of Nutritional Biochemistry,1991,2(2):92-96.  

[41] PACK M,RODEHUTSCORD M,JACOBS S,et al.Amino acid requirements of rainbow trout (Oncorhynchusmykiss) Ⅱ.Protein deposition as function of dietary methionine,threonine and arginine[J].Journal of Applied Ichthyology,1995,11(3/4):390-393.
文章导航

/