动物营养学报 >

2019 , Vol. 31 >Issue 5: 2232 - 2242

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.05.030

实验动物营养 Laboratory animal nutrition

枯草芽孢杆菌铜对先天性缺铜大鼠生长发育、器官指数、铜沉积量、血清生化和抗氧化指标的影响

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  • 1. 青岛农业大学食品科学与工程学院, 青岛 266109;
    2. 国家水禽产业技术体系营养与饲料功能研究室, 青岛 266109
刘国栋(1994-),男,山东安丘人,硕士研究生,研究方向为营养与保健。E-mail:1373657529@qq.com

收稿日期: 2018-10-15

  网络出版日期: 2019-05-15

基金资助

国家水禽产业技术体系专项基金（CARS-42-14）；国家重点研发计划"绿色水禽高效安全养殖技术应用与示范"（2018YFD0501501）

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Influences of Bacillus subtilis Copper on Growth and Development, Organ Indexes, Copper Deposition and Serum Biochemical and Antioxidant Indices of Congenital Copper Deficiency Rats

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  • 1. College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China;
    2. National Waterfowl Industrial Technology System Nutrition and Feed Function Laboratory, Qingdao 266109, China

Received date: 2018-10-15

  Online published: 2019-05-15

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摘要

本试验旨在研究枯草芽孢杆菌铜对先天性缺铜大鼠生长发育、器官指数、铜沉积量、血清生化和抗氧化指标的影响。试验分为模型期和干预试验期。模型期选取怀孕10 d的SD大鼠72只，平均分为模型组和对照组，每组6个重复，每个重复6只。模型组大鼠孕期饲喂缺铜饲粮（铜含量为0.33 mg/kg），对照组大鼠孕期饲喂正常饲粮（在缺铜饲粮基础上添加6.0 mg/kg五水硫酸铜），持续到哺乳期结束，验证建模是否成功。干预试验期选择24日建模成功的缺铜幼龄大鼠108只，随机分成6个试验组，Ⅰ~Ⅲ组为硫酸铜组（缺铜饲粮基础上分别添加3.0、6.0和9.0 mg/kg五水硫酸铜），Ⅳ~Ⅵ组为枯草芽孢杆菌铜组（缺铜饲粮基础上分别添加3.0、6.0和9.0 mg/kg枯草芽孢杆菌铜），每个组3个重复，每个重复6只。试验期35 d。结果表明：1）在铜添加量相同条件下，Ⅳ组体重和血清谷丙转氨酶（ALT）、谷胱甘肽过氧化物酶（GSH-Px）活性显著或极显著高于Ⅰ组（P<0.05或P<0.01），Ⅳ组血清谷草转氨酶/谷丙转氨酶（AST/ALT）显著低于Ⅰ组（P<0.05）。Ⅴ组血清铜蓝蛋白（CP）、碱性磷酸酶（AKP）活性显著或极显高于Ⅱ组（P<0.05或P<0.01），Ⅴ组血清丙二醛（MDA）含量显著低于Ⅱ组（P<0.05）。Ⅵ组血清AKP活性和肾脏中铜沉积量显著或极显高于Ⅲ组（P<0.05或P<0.01）。2）在硫酸铜组中，Ⅱ、Ⅲ组体重、体长显著高于Ⅰ组（P<0.05），Ⅲ组血清CP活性显著或极显著高于Ⅰ、Ⅱ组（P<0.05或P<0.01），Ⅲ组血清AKP活性显著高于Ⅱ组（P<0.05），Ⅲ组血清谷草转氨酶（AST）、GSH-Px和铜锌超氧化物歧化酶（CuZn-SOD）活性及肾脏中铜沉积量显著或极显著高于Ⅰ组（P<0.05或P<0.01），Ⅲ组血清MDA含量极显著低于Ⅰ组（P<0.01）。在枯草芽孢杆菌铜组中，Ⅵ组体重、体长，血清AST、GSH-Px和CuZn-SOD活性，心脏、肝脏、肾脏中铜沉积量显著或极显著高于Ⅳ组（P<0.05或P<0.01），Ⅵ组血清CP和AKP活性显著或极显著高于Ⅳ、Ⅴ组（P<0.05或P<0.01）。由此可见，枯草芽孢杆菌铜能提高先天性缺铜大鼠体重，血清CP、AKP、ALT活性，提高机体抗氧化能力，增加肝脏、肾脏中铜沉积量，效果优于硫酸铜，且降低了饲粮中铜添加量。

关键词： 枯草芽孢杆菌铜; 大鼠; 先天性缺铜; 生长发育; 血清生化指标; 抗氧化; 铜沉积

本文引用格式

刘国栋, 王宝维, 黄燕萍, 葛文华, 张名爱, 岳斌, 高凯, 孔敏 . 枯草芽孢杆菌铜对先天性缺铜大鼠生长发育、器官指数、铜沉积量、血清生化和抗氧化指标的影响[J]. 动物营养学报, 2019 , 31(5) : 2232 -2242 . DOI: 10.3969/j.issn.1006-267x.2019.05.030

Abstract

This experiment was conducted to study the influences of Bacillus subtilis copper on growth and development, organ indices, copper deposition and serum biochemical and antioxidant parameters of congenital copper deficiency rats. The experiment was divided into model period and intervention period. In the model period, seventy-two pregnant SD rats were divided into 2 groups (model group and control group) with 6 replicates per group and 6 replicates per replicate. The pregnancy rats in the model group were fed a low copper diet (copper content was 0.33 mg/kg), and the others in the control group were fed a normal diet (low copper diets supplemented with 6.0 mg/kg copper sulfate pentahydrate), continue until the end of the lactation period and to verify that the model was successful. In the intervention period, a total of 108 successfully modeled copper deficiency young rats were randomly divided into 6 groups, groups Ⅰ to Ⅲ were copper sulfate groups (low copper diets supplemented with 3.0, 6.0 and 9.0 mg/kg copper sulfate pentahydrate, respectively), and groups Ⅳ to Ⅵ were Bacillus subtilis copper groups (low copper diets supplemented with 3.0, 6.0 and 9.0 mg/kg Bacillus subtilis copper, respectively), each group had 3 replicates and each replicate had 6 rates. The experiment lasted for 35 days. The results showed as follows: 1) under the same copper addition condition, the body weight and activities of glutamic-pyruvic transaminase (ALT) and glutathione peroxidase (GSH-Px) in serum of group Ⅳ were significantly higher than those of group Ⅰ (P < 0.05 or P < 0.01), and the serum glutamic-oxaloacetic transaminase/glutamic-pyruvic transaminase (AST/ALT) of group Ⅳ was significantly lower than that of group Ⅰ (P < 0.05). The activities of ceruloplasmin (CP) and alkaline phosphatase (AKP) in serum of group Ⅴ were significantly higher than those of group Ⅱ (P < 0.05 or P < 0.01), and the serum malonaldehyde (MDA) content of group Ⅴ was significantly lower than that of group Ⅱ (P < 0.05). The serum AKP activity and kidney copper deposition of group Ⅵ were significantly higher than those of group Ⅲ (P < 0.05 or P < 0.01). 2) In copper sulfate groups, the body weight and body length of groups Ⅱ and Ⅲ were significantly higher than those of group Ⅰ (P < 0.05), the serum CP activity of group Ⅲ was significantly lower than that of groups Ⅰ and Ⅱ (P < 0.05 or P < 0.01), the serum AKP activity of group Ⅲ was significantly lower than that of group Ⅱ (P < 0.05), the activities of glutamic-oxaloacetic transaminase (AST), GSH-Px and Cu-Zn superoxide dismutase (CuZn-SOD) in serum and kidney copper deposition of group Ⅲ were significantly higher than those of group Ⅰ (P < 0.05 or P < 0.01), and the serum MDA content of group Ⅲ was significantly lower than that of group Ⅰ (P < 0.05). In Bacillus subtilis copper groups, the body weight, body length and the activities of AST, GSH-Px and CuZn-SOD in serum and the copper deposition in heart, liver and kidney of group Ⅵ were significantly higher than those of group Ⅳ (P < 0.05 or P < 0.01), the activities of CP and AKP in serum of group Ⅵ were significantly higher than those of groups Ⅳ and Ⅴ (P < 0.05 or P < 0.01). In conclusion, the Bacillus subtilis copper can increase the body weight and the activities of CP, AKP and ALT in serum of congenital copper deficiency rats, improve the body antioxidant capacity, increase the copper deposition in liver and kidney, the effects are better than copper sulfate, and reduce the copper addition in the diet.

Key words： Bacillus subtilis copper; rats; congenital copper deficiency; growth and development; serum biochemical parameters; antioxidant; copper deposition

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