动物营养学报 >

2019 , Vol. 31 >Issue 5: 2243 - 2253

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.05.031

实验动物营养 Laboratory animal nutrition

枯草芽孢杆菌锌对先天性缺锌大鼠免疫性能、抗氧化能力以及生殖器官发育的影响

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  • 1. 青岛农业大学食品科学与工程学院, 青岛 266109;
    2. 国家水禽产业技术体系营养与饲料功能研究室, 青岛 266109
黄燕萍(1993-),女,山东临沂人,硕士研究生,研究方向为营养与保健。E-mail:zipingangel@qq.com

收稿日期: 2018-10-31

  网络出版日期: 2019-05-15

基金资助

国家水禽产业技术体系专项基金（CARS-42-14）；国家重点研发计划"绿色水禽高效安全养殖技术应用与示范"（2018YFD0501501）

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Effects of Bacillus subtilis-Zinc on Immunity Function, Antioxidant Capacity and Reproduction Organ Development of Congenital Zinc Deficiency Rats

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  • 1. College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China;
    2. National Waterfowl Industrial Technology System Nutrition and Feed Function Laboratory, Qingdao 266109, China

Received date: 2018-10-31

  Online published: 2019-05-15

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摘要

本试验以枯草芽孢杆菌锌为研究素材，研究其对先天性缺锌大鼠免疫性能、抗氧化能力以及生殖器官发育的影响，以确定枯草芽孢杆菌锌的作用效果。试验分为2个阶段：先天性缺锌幼龄大鼠模型建立和缺锌大鼠后天干预试验。模型建立阶段：选取同期怀孕雌性SD大鼠72只，分为缺锌组和对照组，每组6个重复，每个重复6只；缺锌组大鼠从怀孕第10天开始饲喂缺锌饲粮（锌含量为13 mg/kg），正常组饲喂正常饲粮（锌含量为38 mg/kg），持续到后代24日龄。干预试验阶段：选择先天缺锌大鼠90只，随机分成5个试验组，分别为硫酸锌组、缺锌组和低、中、高剂量枯草芽孢杆菌锌组，均饲喂缺锌饲粮；选择模型组中正常组18只大鼠作对照组，继续饲喂正常饲粮，每组设3个重复，每个重复6只。试验期5周。结果表明：1）中、高剂量枯草芽孢杆菌锌能够促进缺锌大鼠肝脏、卵巢发育；提高先天性缺锌大鼠血清免疫球蛋白M（IgM）、单核细胞趋化蛋白-1（MCP-1）含量及铜锌超氧化物歧化酶（Cu-Zn SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性；促进卵泡的成熟及雌二醇（E2）、促卵泡生成素（FSH）、促黄体生成素（LH）的分泌。2）中剂量枯草芽孢杆菌锌在促进先天性缺锌大鼠肝脏、卵巢发育与提高免疫性能和抗氧化能力方面的功效优于硫酸锌。枯草芽孢杆菌锌能够促进先天性缺锌大鼠的肝脏、卵巢等器官发育，提高机体的免疫性能和抗氧化能力，从而促进缺锌大鼠生理机能的修复，且其干预效果优于同等锌含量的硫酸锌，可以降低饲粮中锌的添加剂量。

关键词： 枯草芽孢杆菌锌; 大鼠; 先天性缺锌; 免疫性能; 抗氧化能力; 生殖器官发育

本文引用格式

黄燕萍, 王宝维, 刘国栋, 葛文华, 张名爱, 岳斌, 王文杰 . 枯草芽孢杆菌锌对先天性缺锌大鼠免疫性能、抗氧化能力以及生殖器官发育的影响[J]. 动物营养学报, 2019 , 31(5) : 2243 -2253 . DOI: 10.3969/j.issn.1006-267x.2019.05.031

Abstract

Bacillus subtilis-zinc was used as a material in this study, and the effects of it on immunity function, antioxidant capacity and reproductive organ development of congenital zinc deficiency rats were investigated. The trial was divided into two stages: establishment of congenital zinc deficiency model and the intervention stage of zinc-deficient rats. Model establishment stage: a total of 72 SD rats in the same late pregnancy period were selected and divided into zinc deficiency group and control group with 6 replicates per group and 6 rats per replicate. The rats in the zinc deficiency group were fed a low-zinc diet (zinc content was 13 mg/kg) from 10 days after pregnancy, and those in the control group were fed a normal diet (zinc content was 38 mg/kg), until the offspring rats were 24 days of age. After that, intervention trial stage: a total of 90 rats with congenital zinc deficiency were randomly divided into 5 groups: zinc deficiency group, ZnSO4 group and low, medium and high doses of Bacillus subtilis-zinc groups, all rats were fed low-zinc diets; 18 offspring rats from the control group were randomly selected and used as a control group to feed the normal diet. Each group had 3 replicates, 6 rats in each replicate. The experiment lasted for 5 weeks. The results showed as follows: 1) the medium and high dose of Bacillus subtilis-zinc could promote the development the kidney and ovary of congenital zinc deficiency rats, increase the contents of immunoglobulin M (IgM), monocyte chemoattractant protein-1 (MCP-1) and the activites of zinc superoxide dismutase (Cu-Zn SOD), glutathione peroxidase (GSH-Px) in serum, contribute to the maturation of follicles, the secretion of sex hormones such as estradiol (E2), follicle-stimulating hormone (FSH) and luteinizing hormone (LH). 2) The medium-dose of Bacillus subtilis-zinc was superior to inorganic zinc in promoting liver and ovary organ development, improving immune function and antioxidant capacity in rats with congenital zinc deficiency. Bacillus subtilis-zinc can promote the development of liver, ovary and other organs in rats with congenital zinc deficiency, improve immune function and antioxidant capacity, thereby promoting the repair of physiological functions of zinc-deficient rats, and its intervention effect is better than the equivalent zinc content of ZnSO4, and can reduce the amount of zinc added to the diet.

Key words： Bacillus subtilis-zinc; rats; congenital zinc deficiency; immunity function; antioxidant capacity; reproductive organ development

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