动物营养学报 >

2019 , Vol. 31 >Issue 5: 2357 - 2366

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.05.043

饲料科学与技术 Feed science and technology

白酒糟酵母培养物对肉仔鸡生长性能、血清抗氧化指标和肠道形态结构的影响

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  • 1. 中国农业科学院饲料研究所, 农业部饲料生物技术重点开放实验室, 北京 100081;
    2. 路德生物环保技术(古蔺)有限公司, 泸州 646509
周建民(1993-),男,山东威海人,硕士研究生,从事单胃动物营养研究。E-mail:zjmspeed@163.com

收稿日期: 2018-10-24

  网络出版日期: 2019-05-15

基金资助

北京市家禽创新团队（BAIC04-2018）；中国农业科学院重大产出科研选题（CAAS-ZDXT2018008-1）

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Effects of Yeast Culture Using Distiller’s Grains on Growth Performance, Serum Antioxidant Indices and Intestinal Morphology of Broilers

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  • 1. Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, The Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    2. LOAD Biological Environmental Technology(Gulin) Co., Ltd., Luzhou 646509, China

Received date: 2018-10-24

  Online published: 2019-05-15

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摘要

本试验旨在研究白酒糟酵母培养物（DGYC）对肉仔鸡生长性能、血清抗氧化指标和肠道形态结构的影响，以确定肉仔鸡饲粮中DGYC的适宜添加水平。选取525只1日龄健康的爱拔益加肉仔鸡公雏，随机分为5个组，每组7个重复，每个重复15只鸡。对照组饲喂基础饲粮，试验组在基础饲粮基础上分别添加0.25%、0.50%、1.00%和2.00%的DGYC。试验期42 d。结果表明：1）与对照组相比，饲粮添加1.00%DGYC提高了肉仔鸡生长后期（22~42日龄）平均日增重（0.05≤P<0.10），显著降低了料重比（P<0.05），且均随DGYC添加水平提高呈二次曲线变化（P<0.05）。2）与对照组相比，饲粮添加0.25%DGYC显著降低了肉仔鸡21日龄腹脂率（P<0.05），显著提高了42日龄腿肌率（P<0.05）；饲粮添加0.50%和1.00%DGYC有降低肉仔鸡42日龄胸肌滴水损失的趋势（0.05≤P<0.10）。3）饲粮添加DGYC线性提高了肉仔鸡21和42日龄血清谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）活性和总抗氧化能力（T-AOC）（P<0.05）；饲粮添加DGYC二次性提高了肉仔鸡21日龄血清GSH-Px活性以及42日龄血清SOD活性和T-AOC（P<0.05）。与对照组相比，饲粮添加1.00%DGYC显著提高了肉仔鸡21日龄血清GSH-Px活性（P<0.05）；饲粮添加0.50%、1.00%和2.00%DGYC显著提高了肉仔鸡21日龄血清SOD活性（P<0.05），显著提高了肉仔鸡21和42日龄血清T-AOC（P<0.05）；饲粮添加0.50%和1.00%DGYC显著提高了肉仔鸡42日龄血清SOD活性（P<0.05）。4）1.00%DGYC添加组肉仔鸡21日龄空肠绒毛高度和42日龄绒毛高度/隐窝深度（V/C）显著高于对照组（P<0.05），且均随DGYC添加水平提高呈二次性升高（P<0.05）。5）肉仔鸡生长性能、血清抗氧化指标和空肠形态结构随DGYC添加水平升高存在二次曲线效应。二次曲线拟合结果表明，DGYC添加水平为0.9%~1.1%时，空肠形态结构最佳；添加水平为1.1%~1.2%时，生长性能和血清抗氧化能力最佳。由此可见，饲粮添加DGYC能提高肉仔鸡血清抗氧化能力，改善空肠黏膜形态结构，从而改善生长性能。以肉仔鸡生长性能、血清抗氧化指标和肠黏膜形态结构为判断指标，推荐肉仔鸡基础饲粮中DGYC添加水平为0.9%~1.2%。

关键词： 白酒糟酵母培养物; 生长性能; 抗氧化指标; 肠道形态结构

本文引用格式

周建民, 马友彪, 张海军, 王晶, 武书庚, 齐广海, 刘建忠, 吕亮 . 白酒糟酵母培养物对肉仔鸡生长性能、血清抗氧化指标和肠道形态结构的影响[J]. 动物营养学报, 2019 , 31(5) : 2357 -2366 . DOI: 10.3969/j.issn.1006-267x.2019.05.043

Abstract

The experiment was to investigate the effects of yeast culture using distiller's grains (DGYC) on growth performance, serum antioxidant indices and intestinal morphology of broilers. A total of 525 one-day-old Arbor Acres (AA) male broilers were randomly allotted into 5 groups with 7 replicates of 15 birds each. The chicks in the control group were fed a basal diet, and those in experimental groups were fed the basal diet supplemented with 0.25%, 0.50%, 1.00% and 2.00% DGYC, respectively. The trial lasted for 42 days. The results showed as follows: 1) compared with the control group, dietary 1.00% DGYC increased the average daily gain of broilers during later growth period (22 to 42 days of age) (0.05≤P<0.10), significantly decreased the ratio of feed to gain of broilers (P<0.05), and dietary DGYC had a quadratic effect on both above indices (P<0.05). 2) Compared with the control group, dietary 0.25% DGYC significantly decreased the abdominal fat rate of broilers at 21 days of age (P<0.05), and significantly increased the leg muscle rate of broilers at 42 days of age (P<0.05); dietary 0.50% and 1.00% DGYC tended to decreased the drip loss in breast muscle of broilers at 42 days of age (0.05≤P<0.10). 3) Dietary DGYC linearly improved the activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) and the total antioxidant capacity (T-AOC) in serum of broilers at both 21 and 42 days of age (P<0.05), and had a quadratic effect on the serum GSH-Px activity of broilers at 21 days of age and the SOD activity and T-AOC in serum of broilers at 42 days of age (P<0.05). Compared with the control group, dietary 1.00% DGYC significantly increased the serum GSH-Px activity of broilers at 21 days of age (P<0.05); dietary 0.50%, 1.00% and 2.00% DGYC significantly increased the serum SOD activity of broilers at 21 days of age (P<0.05), and significantly increased the serum T-AOC of broilers at both 21 and 42 days of age (P<0.05); dietary 0.50% and 1.00% DGYC significantly increased the serum SOD activity of broilers at 42 days of age (P<0.05). 4) The villus height at 21 days of age and the villus height to crypt depth ratio at 42 days of age in jejunum of broilers in 1.00% DGYC supplementation group were significantly higher than those in the control group (P<0.05), and all of them increased quadratically with DGYC supplemental level increasing (P<0.05). 5) Dietary DGYC caused quadratic effects on growth performance, serum antioxidant indices and jejunum morphology of broilers. The predicted optimal DGYC supplemental level for the best jejunum morphology was 0.9% to 1.1%, and for the best growth performance and serum antioxidant capacity was 1.1% to 1.2%. These results indicate that dietary DGYC can increase serum antioxidant capacity, improve morphology of jejunal mucosa, and enhance the growth performance of broilers. Based on the growth performance, serum antioxidant indices and intestinal mucosal morphology of broilers, the DGYC supplemental level in the basal diet for broilers is recommended to be 0.9% to 1.2%.

Key words： yeast culture using distiller's grains; growth performance; antioxidant indices; intestinal morphology

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