饲料科学与技术 Feed science and technology

不同功能性寡糖对细菌增殖与黏附性影响

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  • 河南农业大学牧医工程学院, 郑州 450002
杜晨红(1991-),女,河南柘城人,硕士研究生,动物营养与饲料科学专业。E-mail:duchenhong8800@163.com

收稿日期: 2018-11-26

  网络出版日期: 2019-05-15

基金资助

河南省重大科技专项子课题(141100110100)

Effects of Different Functional Oligosaccharides on Bacteria Proliferation and Adhesion

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  • College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China

Received date: 2018-11-26

  Online published: 2019-05-15

摘要

本试验旨在研究不同功能性寡糖对乳酸杆菌、大肠杆菌和沙门氏菌增殖的影响及大肠杆菌、沙门氏菌对猪空肠上皮细胞(IPEC-J2)黏附性的影响。试验用8种功能性寡糖以0.25%的添加量分别增殖干酪乳杆菌、植物乳杆菌、大肠杆菌和沙门氏菌,测定其对增殖的影响;并以IPEC-J2作为体外模型,通过菌落计数的方法,研究不同功能性寡糖对大肠杆菌、沙门氏菌黏附IPEC-J2的影响。结果表明:1)8种功能性寡糖对细菌的增殖作用有差异,果寡糖对有益菌的增殖效果最显著,且对有害菌增殖效果最低并有一定抑制生长的趋势。2)8种功能性寡糖均能显著降低大肠杆菌和沙门氏菌对IPEC-J2的黏附性(P<0.05),其中甘露寡糖对沙门氏菌的黏附抑制作用最好,果寡糖则对大肠杆菌黏附抑制作用最明显。由此可见,在本试验条件下,功能性寡糖不仅能够促进有益菌大量增殖,而且对有害菌增殖也有一定促进作用,且不同功能性寡糖对细菌的促进作用差异显著;8种功能性寡糖能够显著抑制大肠杆菌、沙门氏菌对肠道上皮细胞黏附的作用。

本文引用格式

杜晨红, 杨东吉, 朱随亮, 刘倩, 张静静, 张晓图, 王志祥 . 不同功能性寡糖对细菌增殖与黏附性影响[J]. 动物营养学报, 2019 , 31(5) : 2378 -2387 . DOI: 10.3969/j.issn.1006-267x.2019.05.045

Abstract

This experiment was conducted to study the effects of different oligosaccharides on the proliferation of Lactobacillus, Escherichia coli and Salmonella, and the influence of Escherichia coli and Salmonella on the adhesion of pig jejunal epithelial cells (IPEC-J2).The effects of 8 functional oligosaccharides on the proliferation of Lactobacillus casei, Lactobacillus plantarum, Escherichia coli and Salmonella were determined with 0.25% addition. The effects of different functional oligosaccharides on the adhesion of Escherichia coli and Salmonella to IPEC-J2 were studied by using IPEC-J2 as an in vitro model and colony counting method. The results showed as follows: 1) there were significant differences in the proliferation of bacteria between the 8 functional oligosaccharides. Among them, fructo-oligosaccharides had the best proliferative effect on the two beneficial bacteria, while it had the lowest proliferative effect on pathogenic bacteria and had a trend to inhibit the growth. 2) Eight functional oligosaccharides could significantly reduce the adhesion of Escherichia coli and Salmonella to IPEC-J2 (P<0.05). Among them, manna oligosaccharides had the best inhibitory effect on the adhesion of Salmonella, while fructo-oligosaccharides had the most obvious effect on Escherichia coli. It is concluded that, in the experiments, functional oligosaccharides can not only promote the proliferation of beneficial bacteria, but also harmful bacteria, and the effects of different functional oligosaccharides on bacteria are significantly different; in addition, 8 functional oligosaccharides can significantly inhibit the adhesion of Escherichia coli and Salmonella to intestinal epithelial cells.

参考文献

[1] 陈惠娜,王蜀金,郭春华,等.功能性寡糖在仔猪中的作用机制及在仔猪饲养中的应用效果[J].饲料研究,2015(5):19-23.

[2] FLICKINGER E,WOLF B K,CHOW J,et al.Glucose-based oligosaccharides exhibit different in vitro fermentation patterns and affect in vivo apparent nutrient digestibility and microbial populations in dogs[J].Journal of Nutrition,2000,130(5):1267-1273.  

[3] ZHAO C,WU Y J,LIU X Y,et al.Functional properties,structural studies and chemo-enzymatic synthesis of oligosaccharides[J].Trends in Food Science & Technology,2017,66:135-145.

[4] SELIM K M,REDA R M.Beta-glucans and mannan oligosaccharides enhance growth and immunity in Nile Tilapia[J].North American Journal of Aquaculture,2015,77(1):22-30.  

[5] BARRANGOU R,ALTERMANN E,HUTKINS R,et al.Functional and comparative genomic analyses of an operon involved in fructooligosaccharide utilization by Lactobacillus acidophilus[J].Proceedings of the National Academy of Sciences of the United States of America,2003,100(15):8957-8962.  

[6] 袁缨,闫际平,陈立华,等.不同寡糖对肉仔鸡肠道主要菌群和免疫器官指数的影响[J].中国饲料,2007(15):15-17.

[7] 李梅,刘文利,赵桂英,等.不同寡糖对仔猪免疫力和生产性能的影响研究[J].安徽农业科学,2010,38(28):15655-15657.

[8] 马岩.木二糖与木三糖分离纯化及其对鸡肠道细菌增殖和细胞黏附率的影响[D].硕士学位论文.广州:华南农业大学,2015.

[9] EBERSBACH T,ANDERSEN J B,BERGSTRÖM A,et al.Xylo-oligosaccharides inhibit pathogen adhesion to enterocytes in vitro[J].Research in Microbiology,2012,163(1):22-27.  

[10] ZHOU X L,KONG X F,LIAN G Q,et al.Dietary supplementation with soybean oligosaccharides increases short-chain fatty acids but decreases protein-derived catabolites in the intestinal luminal content of weaned Huanjiang mini-piglets[J].Nutrition Research,2014,34(9):780-788.  

[11] COTTER P A,MILLER J F.Triggering bacterial virulence[J].Science,1996,273(5279):1183-1185.  

[12] 范小兵,周丛,杭晓敏,等.昂立植物乳杆菌粘附肠上皮细胞的研究[J].中国微生态学杂志,2004,16(6):353-355.

[13] GHASEMIAN M,JAHANIAN R.Dietary mannan-oligosaccharides supplementation could affect performance,immunocompetence,serum lipid metabolites,intestinal bacterial populations,and ileal nutrient digestibility in aged laying hens[J].Animal Feed Science and Technology,2016,213:81-89.

[14] SLAVIN J.Fiber and prebiotics:mechanisms and health benefits[J].Nutrients,2013,5(4):1417-1435.  

[15] GÄNZLE M G,FOLLADOR R.Metabolism of oligosaccharides and starch in lactobacilli:a review[J].Frontiers in Microbiology,2012,3:340.

[16] NAGPAL R,KAUR A.Synbiotic effect of various prebiotics on in vitro activities of probiotic lactobacilli[J].Ecology of Food and Nutrition,2011,50(1):63-68.  

[17] 房晓,段荣帅,王凤山.低聚糖对乳杆菌生物活性的作用[J].药物生物技术,2014(4):338-342.

[18] CARDELLE-COBAS A,CORZO N,OLANO A,et al.Galactooligosaccharides derived from lactose and lactulose:influence of structure on Lactobacillus,Streptococcus and Bifidobacterium growth[J].International Journal of Food Microbiology,2011,149(1):81-87.  

[19] KAPLAN H,HUTKINS R W.Fermentation of fructooligosaccharides by lactic acid bacteria and bifidobacteria[J].Applied and Environmental Microbiology,2000,66(6):2682-2684.  

[20] 潘晓东.若干寡糖的功能特性及对肠道生理生态调控机制的研究[D].博士学位论文.杭州:浙江大学,2009.

[21] 孙芝兰.卷曲乳杆菌益生特性及黏附机理研究[D].博士学位论文.济南:山东大学,2012.

[22] ORTIZ G G.Natural sources against veterinary pathogens:evaluation of the anti-adhesive and anti-biofilm activity of wheat bran[J].Canadian Review of Sociology/revue Canadienne De Sociologie,2013,23(1):97-117.

[23] ZOU P,YANG X,WANG J,et al.Advances in characterisation and biological activities of chitosan and chitosan oligosaccharides[J].Food Chemistry,2016,190:1174-1181.

[24] COPPA G V,ZAMPINI L,GALEAZZI T,et al.Human milk oligosaccharides inhibit the adhesion to Caco-2 cells of diarrheal pathogens:Escherichia coli,Vibrio cholerae,and Salmonella fyris[J].Pediatric Research,2006,59(3):377-382.  

[25] 王凤英,王玉梅,常青,等.母乳及婴儿食品对致病性大肠杆菌粘附的影响[J].第三军医大学学报,2001,23(4):478-480.

[26] COZENS D,READ R C.Anti-adhesion methods as novel therapeutics for bacterial infections[J].Expert Review of Anti-Infective Therapy,2012,10(12):1457-1468.  
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