饲粮中添加沙棘果渣对体外瘤胃发酵参数和营养物质有效降解率的影响

刁小高, 郝小燕, 赵俊星, 丁娜, 于胜晨, 项斌伟, 张文佳, 张建新

doi:10.3969/j.issn.1006-267x.2019.05.050

动物营养学报 >

2019 , Vol. 31 >Issue 5: 2423 - 2430

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.05.050

研究简报 Short communications

饲粮中添加沙棘果渣对体外瘤胃发酵参数和营养物质有效降解率的影响

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1. 山西农业大学动物科技学院, 太谷 030801;
2. 山西省右玉县畜牧局, 右玉 037200

刁小高(1991-),男,河南夏邑人,硕士研究生,从事反刍动物营养与饲料科学研究。E-mail:237640529@qq.com

收稿日期: 2018-11-21

网络出版日期: 2019-05-15

基金资助

国家现代肉羊技术体系（CARS-38）；山西省协同创新中心项目；国家星火计划项目（2015GA630004）；山西省重点研发计划项目（201603D21107-2）

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Effects of Dietary Sea Buckthorn Pomace on Rumen Fermentation Parameters in Vitro and Effective Degradability of Nutrients

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1. College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China;
2. Animal Husbandry Bureau of Youyu County, Youyu 037200, China

Received date: 2018-11-21

Online published: 2019-05-15

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摘要

本试验旨在研究饲粮中添加沙棘果渣（SBP）对体外瘤胃发酵参数和营养物质有效降解率的影响。试验选用6只健康、1.5岁左右、体重30~45 kg、安装瘤胃瘘管的杜×寒杂交F1肉羊羯羊。试验采用体外产气法和尼龙袋法对添加不同水平SBP[0（对照组）、8%（8SBP组）、16%（16SBP组）、24%（24SBP组）]的全混合日粮72 h产气量、瘤胃液pH、氨态氮（NH₃-N）、挥发性脂肪酸（VFA）、微生物蛋白（MCP）浓度以及营养物质有效降解率进行测定。结果表明：1）16SBP和24SBP组的72 h产气量和潜在产气量均显著高于对照组（P<0.05），SBP的添加对产气速率没有产生显著的影响（P>0.05）。2）发酵液pH随着SBP添加水平的提高而降低，24SBP组pH最低，显著低于其他各组（P<0.05）；与对照组相比，各试验组发酵液NH₃-N和MCP浓度都有上升的趋势，但差异不显著（P>0.05）；甲烷（CH₄）和二氧化碳（CO₂）产量也有所变化，但没有产生显著差异（P>0.05）；试验组乙酸和总VFA浓度均显著高于对照组（P<0.05）。3）与对照组相比，16SBP和24SBP组干物质（DM）、酸性洗涤纤维（NDF）有效降解率显著提高（P<0.05），8SBP和16SBP组粗蛋白质（CP）有效降解率显著提高（P<0.05）。综上，饲粮中添加SBP改善了体外发酵并且提高了营养物质的降解率，但饲粮中最适SBP添加水平还需要进一步通过试验确定。

关键词： 沙棘果渣; 体外产气法; 尼龙袋法; 产气量; 有效降解率

本文引用格式

刁小高, 郝小燕, 赵俊星, 丁娜, 于胜晨, 项斌伟, 张文佳, 张建新 . 饲粮中添加沙棘果渣对体外瘤胃发酵参数和营养物质有效降解率的影响[J]. 动物营养学报, 2019 , 31(5) : 2423 -2430 . DOI: 10.3969/j.issn.1006-267x.2019.05.050

Abstract

The purpose of this experiment was to investigate the effects of dietary sea buckthorn pomace (SBP) on rumen fermentation parameters and effective degradability of nutrients in vitro. Six healthy, 1.5-year-old, weight of 30 to 45 kg Dorper×thin-tailed Han crossbred Capricorn meat sheep F₁ with installed rumen fistulas were selected. In vitro gas production method and nylon bag method were used to determine the 72 h gas production, rumen fluid pH, ammoniacal nitrogen (NH₃-N), volatile fatty acids (VFA), microbe protein (MCP) concentrations and the effective degradability of nutrients of total mixed ration with SBP at different supplemental levels of 0 (control group), 8% (8SBP group), 16% (16SBP group) and 24% (24SBP group), respectively. The results showed as follows: 1) 72 h gas production and potential gas production in 16SBP and 24SBP groups were significantly higher than those in the control group (P<0.05), but adding SBP had no significant effect on the gas production rate (P>0.05). 2) The pH decreased with the increase of SBP supplement level, and the 24SBP group was the lowest (P<0.05). Compared with the control group, the NH₃-N and MCP concentrations in experimental groups had an increased trend, but the difference was not significant (P>0.05); methane (CH₄) and CO₂ production were affected, but there were no significant differences among groups (P>0.05). The concentrations of acetic acid and total VFA in experimental groups were significanlty higher than those in the control group (P<0.05). 3) Compared with the control group, the effective degradability of dry matter and acid detergent fiber in 16SBP and 24SBP groups significantly increased (P<0.05), and the effective degradability of crude protein in 8SBP and 16SBP groups significantly increased (P<0.05). It is concluded that the addition of SBP to the diet can improve in vitro fermentation and the degradation rate of nutrients, but the optimal supplemental level of SBP in the diet needs to be further determined by feeding experiments.

Key words： sea buckthorn pomace; in vitro gas production method; nylon bag method; gas production; effective degradability

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