猪与禽营养 Swine and poultry nutrition

饲喂频率对哺乳仔猪生长性能和骨骼肌蛋白质合成的影响

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  • 1. 西南科技大学生命科学与工程学院, 绵阳 621010;
    2. 中国农业科学院北京畜牧兽医研究所, 动物营养学国家重点实验室, 北京 100193
刘静波(1985-),男,四川德阳人,副研究员,博士,主要从事单胃动物营养和分子营养研究。E-mail:liuswust@163.com

收稿日期: 2019-01-02

  网络出版日期: 2019-07-19

基金资助

四川省科技计划资助(2018JY0225);四川省教育厅资助科研项目(17ZA0413)

Effects of Meal Frequency on Growth Performance and Muscle Protein Synthesis in Suckling Piglets

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  • 1. School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China;
    2. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2019-01-02

  Online published: 2019-07-19

摘要

本试验旨在比较饲喂频率对哺乳仔猪生长性能、胴体组成和骨骼肌蛋白质合成相关基因表达的影响。试验选用来自于4头母猪的16头4日龄的"杜×长×大"仔猪,按体重和性别配对成8对,每对仔猪随机分配到每天饲喂6次组(M6组)和每天饲喂12次组(M12组),每组8头猪,每个配对内仔猪采食量保持一致,人工乳饲喂21 d。结果表明:1)与M12组相比,M6组仔猪的末重,第2周、第3周和全期的平均日增重显著提高(P<0.05),第2周、第3周和全期的料重比显著降低(P<0.05)。2)与M12组相比,M6组仔猪胴体瘦肉率、背最长肌重和半腱肌重显著提高(P<0.05)。3)与M12组相比,M6组仔猪背最长肌胰岛素样生长因子1(IGF1)基因表达量有提高的趋势(P=0.05);与M12组相比,仔猪背最长肌胰岛素样生长因子1受体(IGF1R)、哺乳动物雷帕霉素靶蛋白(mTOR)、真核细胞翻译起始因子4E(EIF4E)和核糖体蛋白S6(RPS6)的mRNA表达量显著提高(P<0.05),ⅡB型活化素受体(ACVR2B)和Ⅰ型活化素受体样激酶5(ALK5)的mRNA表达量显著降低(P<0.05);与M12组相比,M6组仔猪背最长肌肌肉生长抑素(MSTN)mRNA表达量没有显著变化(P>0.05)。综上所述,在本试验条件下,降低饲喂频率可促进胴体瘦肉沉积和骨骼肌生长,主要通过上调IGF1-IGF1R-mTOR通途路下调MSTN受体表达来实现。

本文引用格式

刘静波, 曹山川, 杨勇, 张宏福 . 饲喂频率对哺乳仔猪生长性能和骨骼肌蛋白质合成的影响[J]. 动物营养学报, 2019 , 31(7) : 3049 -3057 . DOI: 10.3969/j.issn.1006-267x.2019.07.015

Abstract

The present study was conducted to determine the effects of meal frequency on growth performance, carcass composition and muscular protein synthesis-related genes expression in suckling piglets. Sixteen piglets of a crossbred genotype[Duroc×(Landrace×Large White)] were obtained from 4 litters in 4 successive replicates and grouped into 8 pairs according to sex and body weight. At 4 days of age, piglets within each pair were offered the same amount of feed allowance either in 6 (M6 group, n=8) or 12 (M12 group, n=8) meals per day during a 3-week interventional period. The results showed as follows:1) compared with M12 group, the final body weight and average daily body weight at week 2, week 3 and during the whole trial in M6 group significantly increased (P<0.05), and the feed/gain at week 2, week 3 and during the whole trial significantly decreased (P<0.05). 2) Compared with M12 group, the carcass lean percentage and weight of longissimus muscle and semitendinosus muscle in M6 group significantly increased (P<0.05). 3) Compared with M12 group, the mRNA expression of insulin-like growth factor 1 (IGF1R) in M6 group had an increasing trend (P=0.05); compared with M12 group, the mRNA expression of insulin-like growth factor 1 receptor (IGF1R), mammalian target of rapamcin (mTOR), eukaryotic cells translate initiation factor 4E (EIF4E) and ribosomal protein S6 (RPS6) in M6 group significantly increased (P<0.05), and the mRNA expression of Ⅱ type B activated receptor (ACVR2B) and type Ⅰ activated kinase receptor (ALK5) in M6 group significantly decreased (P<0.05); compared with M12 group, the mRNA expression of myostatin (MSTN) in M6 group had no significant change (P>0.05). Collectively, reduced meal frequency results in accelerated carcass lean deposition and muscle hypotrophy, which may be mediated by activated IGF1-IGF1R-mTOR pathway and down-regulated expression of MSTN receptors.

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