动物营养学报 >

2019 , Vol. 31 >Issue 7: 3188 - 3196

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.07.030

反刍与草食动物营养 Ruminant and herbivore nutrition

补喂L-精氨酸或N-氨甲酰谷氨酸对伊犁马运动性能和血浆生化指标的影响

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  • 新疆农业大学动物科学学院, 新疆肉乳用草食动物营养重点实验室, 乌鲁木齐 830052
张仕琦(1993-),男,新疆额敏人,硕士研究生,研究方向草食动物营养代谢。E-mail:1505521966@qq.com

收稿日期: 2018-12-18

  网络出版日期: 2019-07-19

基金资助

自治区"十三五"重大专项-运动马健康养殖共性技术研发（2017A01002-2-01）

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Effects of Supplemented with L-Arginine or N-Carbamylglutamate on Athletic Performance and Plasma Biochemical Indexes of Yili Horses

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  • Xinjiang Key Laboratory of Meat & Milk Production Herbivore Nutrition, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China

Received date: 2018-12-18

  Online published: 2019-07-19

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摘要

本试验旨在探究补喂L-精氨酸或N-氨甲酰谷氨酸对伊犁马运动性能和血浆生化指标的影响。选取15匹年龄为2岁、体重[（388.88±23.24）kg]相近并经过严格训练的速度赛用伊犁马，分为3组，每组5匹（3♂+2♀），每日进行等强度训练。对照组补喂L-丙氨酸，试验Ⅰ组补喂L-精氨酸，试验Ⅱ组补喂N-氨甲酰谷氨酸，进行为期4周的试验。在试验第28天进行3 600 m速度赛，并采集血液样品。结果表明：与对照组相比，试验Ⅰ组、试验Ⅱ组试验初始和试验第28天时的3 600 m速度赛平均速度差异不显著（P>0.05）；试验Ⅰ组、试验Ⅱ组血浆一氧化氮含量分别较对照组升高了27.53%（P<0.01）、18.10%（P<0.01），且试验Ⅰ组较试验Ⅱ组升高了7.99%（P<0.01）；试验Ⅰ组、试验Ⅱ组血浆氨含量分别较对照组降低了25.44%（P<0.01）、14.04%（P<0.01），且试验Ⅰ组较试验Ⅱ组降低了13.27%（P<0.01）；血浆5-羟色胺、葡萄糖、丙酮酸含量各组间无显著差异（P>0.05）；与对照组相比，试验Ⅰ组血浆乳酸含量极显著降低（P<0.01），试验Ⅱ组则无显著变化（P>0.05）；试验Ⅰ组、试验Ⅱ组血浆过氧化氢酶活性分别较对照组升高了24.00%（P<0.01）、21.33%（P<0.01）；试验Ⅰ组、试验Ⅱ组血浆抗羟基自由基能力分别较对照组升高了5.48%（P<0.05）、8.47%（P<0.05）。因此，补喂L-精氨酸或N-氨甲酰谷氨酸均可提高伊利马血浆一氧化氮含量和抗氧化能力，降低血浆氨含量，且补喂L-精氨酸还能够降低伊犁马血浆乳酸含量，但二者对伊犁马3 600 m速度赛的平均速度无显著影响。L-精氨酸、N-氨甲酰谷氨酸可作为营养调控剂，用于缓解运动马运动疲劳或促进运动后的疲劳恢复。

关键词： 伊犁马; L-精氨酸; N-氨甲酰谷氨酸; 运动性能; 抗氧化能力

本文引用格式

张仕琦, 李晓斌, 张文杰, 韩明, 王世昌, 杨开伦 . 补喂L-精氨酸或N-氨甲酰谷氨酸对伊犁马运动性能和血浆生化指标的影响[J]. 动物营养学报, 2019 , 31(7) : 3188 -3196 . DOI: 10.3969/j.issn.1006-267x.2019.07.030

Abstract

The aim of this study was to investigate the effects of supplemented with L-arginine or N-carbamylglutamate on athletic performance and plasma biochemical indexes of Yili horses. Fifteen Yili horses with similar body weight[(388.88±23.24) kg] and aged 2 years old, were divided into 3 groups with 5 horses (3♂ +2♀) in each group, and all horses were trained according to the same training intensity every day. Horses in control group were supplemented with L-alanine, horses in trial group Ⅰ were supplemented with L-arginine, and horses in trial group Ⅱ were supplemented with N-carbamyleglutamate. The trial was lasted for 4 weeks. The 3 600 m speed race was conducted and blood samples were collected at the 28th day of trial. The results showed as follows:compared with control group, the average speed of the 36 00 m speed race on initial and day 28th of trial in trial groups Ⅰ and Ⅱ had no significant difference (P>0.05). Plasma nitric oxide content in trial groups Ⅰ and Ⅱ was increased by 27.53% (P<0.01) and 18.10% (P<0.01) compared with control group, respectively, and it in trial group Ⅰ was increased by 7.99% compared with trial group Ⅱ (P<0.01). The plasma ammonia content in trial groups Ⅰ and Ⅱ was decreased by 25.44% (P<0.01) and 14.04% (P<0.01) compared with control group, respectively, and it in trial group Ⅰ was decreased by 13.27% compared with trial group Ⅱ (P<0.01). There was no significant difference in plasma 5-hydroxy tryptamine, glucose and pyruvic acid contents among groups (P>0.05). Compared with control group, plasma lactate content in trial group Ⅰ was significantly decreased (P<0.01), but there was no significant difference in trial group Ⅱ (P>0.05). Compared with control group, plasma catalase activity in trial groups Ⅰ and Ⅱ was increased by 24.00% (P<0.01) and 21.33% (P<0.01), respectively, and plasma hydroxyl radical resistance in trial in groups Ⅰ and Ⅱ was increased by 5.48% (P<0.05) and 8.47% (P<0.05), respectively. Therefore, supplemented with L-arginine or N-carbamylglutamate can both increase plasma nitric oxide content and antioxidant capacity, and decrease plasma ammonia content, and supplemented with L-arginine can also decrease the plasma lactic acid content, but both of them have no significant effect on the average speed of 3 600 m speed race of Yili horses. L-arginine and NCG can be used as nutritional regulators to relieve exercise fatigue or promote fatigue recovery after exercise.

Key words： Yili horses; L-arginine; N-carbamylglutamate; athletic performance; antioxidant capacity

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