动物营养学报 >

2019 , Vol. 31 >Issue 7: 3197 - 3206

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.07.031

水产营养 Aquaculture Nutrition

饲料中添加丙氨酰-谷氨酰胺二肽对黄颡鱼幼鱼生长性能、抗氧化能力、免疫应答能力及抗逆能力的影响

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  • 1. 宁波大学海洋学院, 宁波 315211;
    2. 贵州大学动物科学学院, 贵阳 550025;
    3. 贵州大学高原山地动物遗传育种与繁殖教育部重点实验室, 贵阳 550025
李雪(1995-),女,吉林白山人,硕士研究生,从事水生动物营养与免疫研究。E-mail:1005518588@qq.com

收稿日期: 2018-12-14

  网络出版日期: 2019-07-19

基金资助

国家自然科学基金项目（31872541，31872222）；贵州省自然科学技术基金基础研究计划项目（黔科合基础[2019]1114）

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Effects of Alanyl-Glutamine Dipeptide Supplementation on Growth Performance, Antioxidant Status, Immune Response and Stress Resistance of Juvenile Yellow Catfish (Pelteobagrus fulvidraco)

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  • 1. School of Marine Sciences, Ningbo University, Ningbo 315211, China;
    2. College of Animal Sciences, Guizhou University, Guiyang 550025, China;
    3. Key Laboratory for Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region of Ministry of Education, Guizhou University, Guiyang 550025, China

Received date: 2018-12-14

  Online published: 2019-07-19

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摘要

本试验旨在研究饲料中添加丙氨酰-谷氨酰胺二肽（AGD）对黄颡鱼幼鱼生长性能、抗氧化能力、免疫应答能力及抗逆能力的影响。以平均体重为（1.98±0.01）g的黄颡鱼幼鱼为研究对象，随机分成5组，每组3个重复，每个重复30尾鱼，分别投喂添加0（对照）、0.25%、0.50%、0.75%和1.00% AGD的5种试验饲料，5种试验饲料中AGD含量的实测值依次为0.08%、0.23%、0.56%、0.69%和1.09%。摄食生长试验持续56 d。结果表明：随着饲料中AGD添加量的增加，试验鱼的增重、特定生长率以及血清总蛋白、白蛋白、球蛋白、补体3和补体4含量与超氧化物歧化酶、过氧化氢酶、谷胱甘肽、溶菌酶、碱性磷酸酶和酸性磷酸酶活性均先增加后降低，0.50%和0.75% AGD组均显著高于对照组（P<0.05）。饲料中添加不同水平的AGD均显著提高了试验鱼血清总抗氧化能力（P<0.05），显著降低了丙二醛含量（P<0.05）。氨氮胁迫96 h后，对照组试验鱼大脑中氨和谷氨酰胺含量显著高于各AGD添加组（P<0.05），同时累积死亡率也显著高于各AGD添加组（P<0.05）。由此可见，饲料中添加适宜水平的AGD能够提高黄颡鱼幼鱼的生长性能、抗氧化能力、免疫应答能力及抗逆能力；基于特定生长率的二次回归模型拟合，获得黄颡鱼幼鱼饲料中AGD的最适添加量为0.56%；综合考虑生长性能、抗氧化能力、免疫应答能力及抗逆能力，推荐黄颡鱼幼鱼饲料中AGD的添加量为0.50%~0.75%。

关键词： 丙氨酰-谷氨酰胺二肽; 黄颡鱼幼鱼; 生长性能; 抗氧化能力; 免疫应答能力; 抗逆能力

本文引用格式

李雪, 张木子, 黎明, 章倩, 王日昕, 姜海波 . 饲料中添加丙氨酰-谷氨酰胺二肽对黄颡鱼幼鱼生长性能、抗氧化能力、免疫应答能力及抗逆能力的影响[J]. 动物营养学报, 2019 , 31(7) : 3197 -3206 . DOI: 10.3969/j.issn.1006-267x.2019.07.031

Abstract

A 56-day feeding trial was conducted to determine the alanyl-glutamine dipeptide (AGD) supplementation on growth performance, antioxidant status, immune response and stress resistance of juvenile yellow catfish (Pelteobagrus fulvidraco). Juvenile yellow catfish with an average body weight of (1.98±0.01) g were randomly assigned to 5 groups with 3 replicates per group and 30 juvenile fish per replicate. Juvenile fish in the 5 groups were fed 5 experimental diets supplemented with 0 (control) 0.25%, 0.50%, 0.75% and 1.00% AGD, and the measured AGD contents in the 5 experimental diets were 0.08%, 0.23%, 0.56%, 0.69% and 1.09%, respectively. The results showed that the weight gain, specific growth rate, serum total protein, albumin, globulin, complement 3, complement 4 contents and superoxide dismutase, catalase, glutathione, lysozyme, alkaline phosphatase, acid phosphatase activities were firstly increased and then increased with dietary AGD supplemental level increasing, and above indexes in 0.50% and 0.75%AGD groups were significantly higher than those in control group (P<0.05). Diet supplemented different levels of AGD could significantly increase the serum total antioxidant capacity (P<0.05), but significantly decrease the serum malondialdehyde content (P<0.05). After 96 h post-challenge on ammonia exposure, the cumulative mortality, brain ammonia and glutamate contents in control group were significantly higher than those in each AGD supplementation groups (P<0.05). In conclusion, suitable level of AGD supplemented into diets can increase the growth performance, antioxidant status, immune response and stress resistance of juvenile yellow catfish. The optimal dietary AGD supplemental level is estimated to be 0.56% in the diet of juvenile yellow catfish by quadratic regression analysis base on specific growth rate. By a comprehensive consideration of growth performance, antioxidant status, immune response and stress resistance, the supplemental level of 0.50% to 0.75% AGD is recommended in diets of juvenile yellow catfish.

Key words： alanyl-glutamine dipeptide; juvenile yellow catfish; growth performance; antioxidant status; immune response; stress resistance

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