动物营养学报 >

2019 , Vol. 31 >Issue 7: 3242 - 3250

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.07.036

实验方法 Experimental Methods

猪仿生消化中小肠阶段消化酶对饲粮总能与粗蛋白质消化率的贡献

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  • 中国农业科学院北京畜牧兽医研究所, 动物营养学国家重点实验室, 北京 100193
王亚(1991-),男,安徽亳州人,硕士研究生,从事饲料养分生物学效价评定的研究。E-mail:wangya920708@163.com

收稿日期: 2018-12-19

  网络出版日期: 2019-07-19

基金资助

中国农业科学院科技创新工程（ASTIP-IAS07）；广东温氏食品集团股份有限公司横向合作项目（2018-YF-01）

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Contribution of Digestive Enzymes in Simulated Digestion of Small Intestine to Digestibility of Gross Energy and Crude Protein of Feed for Pigs

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  • State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2018-12-19

  Online published: 2019-07-19

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摘要

本试验旨在探讨猪仿生消化中小肠阶段主要消化酶的组成及活性对饲粮养分消化率的贡献，为仿生消化法评定猪饲粮养分的消化率提供参考。试验采用单因素完全随机设计，5个处理分别为胃模拟消化、胃+小肠（淀粉酶）模拟消化、胃+小肠（淀粉酶+糜蛋白酶）模拟消化、胃+小肠（淀粉酶+糜蛋白酶+胰蛋白酶）模拟消化、胃+小肠（淀粉酶+糜蛋白酶+胰蛋白酶+小肠消化4 h时补充消化酶）模拟消化，每个处理5个重复，每个重复1根消化管。考察各处理条件下玉米、豆粕、小麦麸及玉米-豆粕型饲粮的干物质、粗蛋白质及总能消化率的差异和各消化酶的贡献。结果表明：1）模拟小肠液中淀粉酶、糜蛋白酶均可显著提高胃消化后玉米、豆粕、小麦麸及玉米-豆粕型饲粮的干物质、粗蛋白质以及总能的消化率（P<0.05）；胰蛋白酶可以进一步提高胃消化后豆粕、小麦麸的干物质、粗蛋白质以及总能消化率（P<0.05）；补充3种消化酶可以提高对小麦麸粗蛋白质及玉米-豆粕型饲粮干物质、粗蛋白质及总能的消化率（P<0.05）。2）小肠液中消化酶对饲粮养分消化率的贡献上，因饲粮底物的不同而异，其中淀粉酶、糜蛋白酶对总能和粗蛋白质消化率的贡献相对较高，而胰蛋白酶和补充消化酶对饲粮养分消化率的贡献虽然相对较少，但也可以引起消化率显著性差异（P<0.05）。在猪的小肠模拟消化阶段，淀粉酶、糜蛋白酶、胰蛋白酶及补充消化酶对饲粮养分的消化程度均有显著性贡献。

关键词： 猪; 仿生消化; 消化酶; 养分消化率

本文引用格式

王亚, 赵峰, 张虎, 党方昆, 高庆涛, 于耀, 杜中原, 萨仁娜 . 猪仿生消化中小肠阶段消化酶对饲粮总能与粗蛋白质消化率的贡献[J]. 动物营养学报, 2019 , 31(7) : 3242 -3250 . DOI: 10.3969/j.issn.1006-267x.2019.07.036

Abstract

This study was conducted to investigate the contribution of composition and activity of main digestive enzymes in simulated digestion of the small intestine to the digestibility of nutrients in feed for pig, which will provide a basis to improve the procedure of simulated digestion for determining nutrient digestibility of feed for pig. Five treatments consisted of gastric simulated digestion, gastric following amylase for small intestinal simulated digestion, gastric following amylase and chymotrypsin for intestinal simulated digestion, gastric following amylase, chymotrypsin and trypsin for intestinal simulated digestion, gastric following amylase, chymotrypsin, trypsin and supplemented digestive enzymes at 4 h for intestinal simulated digestion were arranged in a single factor completely randomized design. Each treatment contained 5 replicates with 1 digestion tube in each. The differences in the digestibility of dry matter, crude protein and gross energy and the contribution of each digestive enzyme to nutrient digestibility were investigated among various treatment conditions for each of corn, soybean meal, wheat bran and corn-soybean meal diet. The results showed as follows:1) amylase and chymotrypsin in simulated intestinal fluid could significantly increase the digestibilityof dry matter, crude protein and gross energy of corn, soybean meal, wheat bran and corn-soybean meal diet digested by simulated gastric fluid (P<0.05). Trypsin could further increase the digestibility of dry matter, crude protein and gross energy of soybean meal and wheat bran digested by simulated gastric fluid (P<0.05). The supplement of 3 digestive enzymes at 4 h of intestinal digestion could increase the crude protein digestibility of wheat bran, and the digestibility of dry matter, crude protein and gross energy of corn-soybean meal (P<0.05). 2) The contribution of digestive enzymes in the small intestinal fluid to the digestibility of feed nutrients varied depending on the feed substrate. Amylase and chymotrypsin had relative greater contribution to the digestibility of gross energy and crude protein contrast to trypsin and supplemented digestive enzymes. However, trypsin and supplemented digestive enzymes also significantly increased the nutrient digestibility (P<0.05). In the simulation of small intestine digestion for pigs, amylase, chymotrypsin, trypsin and supplemental digestive enzymes have significant contribution to the digestion of feed nutrients.

Key words： pig; simulated digestion; digestive enzyme; nutrient digestibility

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