动物营养学报 >

2019 , Vol. 31 >Issue 7: 3260 - 3267

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.07.038

饲料科学与技术 Feed science and technology

枯草芽孢杆菌对肥育猪血浆生化和免疫指标及粪便菌群的影响

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  • 1. 湖南农业大学动物科学技术学院, 湖南省畜禽安全生产协同创新中心, 长沙 410128;
    2. 朝日可尔必思健康管理(上海)有限公司, 上海 200233;
    3. 上海牧冠企业发展有限公司, 上海 201700
贺长青(1965-),男,湖南祁东人,副教授,硕士,主要研究方向为畜禽安全生产技术。E-mail:2681633710@qq.com

收稿日期: 2018-12-04

  网络出版日期: 2019-07-19

基金资助

畜禽安全生产协同创新中心专项资金（CICAPS）；湖南农业大学产学研合作项目（13098）；湖南省自然科学基金项目（2017JJ2120）

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Effects of Bacillus subtilis on Plasma Biochemical and Immune Indices and Fecal Microflora of Finishing Pigs

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  • 1. Hunan Co-Innovation Center of Animal Production Safety, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
    2. Asahi Calpis Wellness(Shanghai) Co., Ltd., Shanghai 200233, China;
    3. Shanghai Naseco Products Company, Shanghai 201700, China

Received date: 2018-12-04

  Online published: 2019-07-19

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摘要

本试验旨在研究枯草芽孢杆菌（Bacillus subtilis，BS）对肥育猪血浆生化和免疫指标及粪便菌群的影响。选取120头平均体重为（62.90±2.37）kg的"杜×长×大"三元杂交肥育猪，随机分为3个组，每组5个重复，每个重复8头猪。对照组（A组）饲喂基础饲粮，试验组（B、C组）分别在基础饲粮中添加100和200 mg/kg BS。预试期7 d，正试期63 d。结果表明：1）与对照组相比，B、C组肥育猪血浆碱性磷酸酶活性极显著降低（P<0.01），B组血浆球蛋白含量显著降低（P<0.05），血浆白蛋白含量显著提高（P<0.05）；2）C组肥育猪血浆免疫球蛋白A含量和猪圆环病毒抗体水平较对照组极显著提高（P<0.01）；3）饲粮中添加BS对肥育猪血浆和肌肉中内毒素含量均无显著影响（P>0.05）；4）与对照组相比，B、C组粪便中双歧杆菌相对含量显著提高（P<0.05），B组粪便中巨大芽孢杆菌相对含量显著提高（P<0.05），C组粪便中产气荚膜梭菌相对含量极显著降低（P<0.01）。由此可见，饲粮中添加BS可在一定程度上改善肥育猪机体代谢，增强机体免疫功能，优化肠道菌群。

关键词： 枯草芽孢杆菌; 肥育猪; 血浆生化指标; 血浆免疫指标; 粪便菌群

本文引用格式

贺长青, 朱瑾, 匡佑华, 陈继发, 曲湘勇, 郭松长, 今林宽和, 周学彬 . 枯草芽孢杆菌对肥育猪血浆生化和免疫指标及粪便菌群的影响[J]. 动物营养学报, 2019 , 31(7) : 3260 -3267 . DOI: 10.3969/j.issn.1006-267x.2019.07.038

Abstract

The aim of this study was to investigate the effects of Bacillus subtilis (BS) on plasma biochemical and immune indices and fecal microflora of finishing pigs. A total of 120 Duroc×Landrace×Yorkshire crossbred finishing pigs with an average body weight of (62.90±2.37) kg were randomly divided into 3 groups with 5 replicates per group and 8 pigs per replicate. The control group (group A) was fed a basal diet, and the experimental groups (groups B and C) were fed the basal diets supplemented with 100 and 200 mg/kg BS, receptivity. The preparation period was 7 days and formal experiment period was 63 days. The results showed as follows:1) compared with the control group, the plasma alkaline phosphatase activity of finishing pigs in groups B and C was extremely significantly decreased (P<0.01); the plasma globulin content of finishing pigs in group B was significantly decreased (P<0.05) and the plasma albumin content was significantly increased (P<0.05). 2) The plasma immunoglobulin A content and porcine circovirus antibody level of finishing pigs in group C were extremely significantly higher than those in control group (P<0.01). 3) The addition of Bacillus subtilis in diets had no significant effect on the endotoxin content in plasma and muscle of finishing pigs (P>0.05). 4) Compared with the control group, the relative content of Bifidobacteria in faeces of finishing pigs in groups B and C was significantly increased (P<0.05), the relative content of Bacillus megaterium in faeces of finishing pigs in group B was significantly increased (P<0.05), and the relative content of Clostridium perfringens in faeces of finishing pigs in group C was extremely significantly decreased (P<0.01). It can be seen that adding BS to diet can improve metabolism, enhance immune function and optimize intestinal flora of finishing pigs to a certain extent.

Key words： Bacillus subtilis; finishing pigs; plasma biochemical indices; plasma immune indices; fecal microflora

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