竹叶提取物对奶牛泌乳性能、血液常规指标、免疫和抗氧化性能的影响

栗明月, 方洛云, 苏汉书, 李义, 郝磊, 蒋林树, 熊本海

doi:10.3969/j.issn.1006-267x.2019.07.043

动物营养学报 >

2019 , Vol. 31 >Issue 7: 3302 - 3309

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.07.043

饲料科学与技术 Feed science and technology

竹叶提取物对奶牛泌乳性能、血液常规指标、免疫和抗氧化性能的影响

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1. 北京农学院动物科学技术学院, 奶牛营养学北京市重点实验室, 北京 102206;
2. 中国农业科学院北京畜牧兽医研究所, 北京 100193

栗明月(1995-),女,北京人,硕士研究生,从事反刍动物营养研究。E-mail:1848467829@qq.com

收稿日期: 2018-12-24

网络出版日期: 2019-07-19

基金资助

国家自然科学基金（31772629）

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Effects of Bamboo Leaf Extracts on Lactation Performance, Blood Routine Indexes and Immune and Antioxidant Ability of Dairy Cows

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1. Key Laboratory for Dairy Cow Nutrition of Beijing, College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China;
2. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2018-12-24

Online published: 2019-07-19

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摘要

本试验旨在研究竹叶提取物对奶牛泌乳性能、血液常规指标、免疫和抗氧化性能的影响。试验选用20头泌乳日龄、体重、胎次及产奶量相近的中国荷斯坦奶牛，采用完全随机设计原则随机分为4组，每组5个重复，每个重复1头牛。对照组饲喂基础饲粮，试验组分别在基础饲粮中添加30、60、90 g/d的竹叶提取物。预试期14 d，正试期35 d。结果表明：1）与对照组相比，试验组奶牛产奶量均有所升高，但差异不显著（P>0.05）。30 g/d组乳糖率、乳蛋白率和乳脂校正率显著高于对照组（P<0.05），90 g/d组体细胞数显著低于其他各组（P<0.05）。2）60 g/d组血液淋巴细胞数量和含量显著高于对照组（P<0.05），30、60 g/d组血液中性粒细胞数量和含量显著高于对照组（P<0.05）。3）30 g/d组血浆中免疫球蛋白M、免疫球蛋白A、免疫球蛋白G含量均显著高于对照组（P<0.05）。4）试验组血浆中过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化酶活性均显著高于对照组（P<0.05），试验组血浆中丙二醛含量显著低于对照组（P<0.05），但各试验组之间无显著差异（P>0.05）。综上所述，饲粮中添加竹叶提取物能够提高奶牛机体免疫和抗氧化性能。本试验条件下，饲粮中竹叶提取物适宜添加水平为30 g/d。

关键词： 竹叶提取物; 奶牛; 免疫; 抗氧化

本文引用格式

栗明月, 方洛云, 苏汉书, 李义, 郝磊, 蒋林树, 熊本海 . 竹叶提取物对奶牛泌乳性能、血液常规指标、免疫和抗氧化性能的影响[J]. 动物营养学报, 2019 , 31(7) : 3302 -3309 . DOI: 10.3969/j.issn.1006-267x.2019.07.043

Abstract

The aim of this study was to investigate the effects of bamboo leaf extracts on lactation performance, blood routine indexes and immune and antioxidant ability of dairy cows. Twenty Chinese Holstein cows with similar lactation age, body weight, parity and milk yield were selected, and using a completely random design, randomly divided into 4 groups with 5 replicates per group and 1 cow per replicate. Cows in the control group were fed a basal diet, and the others in experimental groups were fed the basal diets supplemented with 30, 60 and 90 g/d bamboo leaf extracts, respectively. The pre-experimental period lasted for 14 days, and the experimental period lasted for 35 days. The results showed as follows:1) compared with the control group, the milk yield of experimental groups were increased, but had no significant difference (P>0.05). The lactose rate, milk protein rate and milk fat correction rate of 30 g/d group were significantly higher than those of the control group (P<0.05), the somatic cell counts of 90 g/d group was significantly lower than that of other groups (P<0.05). 2) The blood lymphocyte number and content of 60 g/d group was significantly higher than that of the control group (P<0.05), the blood neutrophile granulocyte number and content of 30 and 60 g/d groups was significantly higher than that of the control group (P<0.05). 3) The contents of immunoglobulin M, immunoglobulin A and immunoglobulin G in plasma of 30 g/d group were significantly higher than those of the control group (P<0.05). 4) The activities of catalase, superoxide dismutase, glutathione peroxidase in plasma of experimental groups were significantly higher than those of the control group (P<0.05), the plasma malondialdehyde content of experimental groups was significantly higher than that of the control group (P<0.05), but there were no significant differences among all experimental groups (P>0.05). In summary, dietary bamboo leaf extracts can improve the body's immune and antioxidant ability of dairy cows. Under these experimental conditions, the suitable dietary bamboo leaf extracts supplemental level is 30 g/d.

Key words： bamboo leaf extracts; dairy cows; immune; antioxidan

参考文献

[1] WANG J,YUE Y D,JIANG H,et al.Rapid screening for flavone C-glycosides in the leaves of different species of bamboo and simultaneous quantization of four marker compounds by HPLC-UV/DAD[J].International Journal of Analytical Chemistry,2012,2012:205101.

[2] WANG J,YUE Y D,TANG F,et al.TLC screening for antioxidant activity of extracts from fifteen bamboo species and identification of antioxidant flavone glycosides from leaves of Bambusa.textilis McClure[J].Molecules,2012,17(10):12297-12311.

[3] ZHANG Y,XU W Z,WU X Q,et al.Addition of antioxidant from bamboo leaves as an effective way to reduce the formation of acrylamide in fried chicken wings[J].Food Additives & Contaminants,2007,24(3):242-251.

[4] ZHANG Y,ZHANG Y.Study on reduction of acrylamide in fried bread sticks by addition of antioxidant of bamboo leaves and extract of green tea[J].Asia Pacific Journal of Clinical Nutrition,2006,16(Suppl.1):131-136.

[5] NAIR M P,MAHAJAN S,REYNOLDS J L,et al.The flavonoid quercetin inhibits proinflammatory cytokine (tumor necrosis factor alpha) gene expression in normal peripheral blood mononuclear cells via modulation of the NF-κB system[J].Clinical and Vaccine Immunology,2006,13(3):319-328.

[6] SHI D,XU M,REN M,et al.Immunomodulatory effect of flavonoids of blueberry (Vaccinium corymbosum L.) leaves via the NF-κB signal pathway in LPS-stimulated RAW 264.7 cells[J].Journal of Immunology Research,2017:5476903.

[7] HU C,ZHANG Y,KITTS D D.Evaluation of antioxidant and prooxidant activities of bamboo Phyllostachys nigra var.Henonis leaf extract in vitro[J].Journal of Agricultural and Food Chemistry,2000,48(8):3170-3176.

[8] ZHANG S,CHEN J,SUN A D,et al.Protective effects and antioxidant mechanism of bamboo leaf flavonoids on hepatocytes injured by CCl₄[J].Food and Agricultural Immunology,2014,25(3):386-396.

[9] 贾可敬.竹叶黄酮提取、纯化及抗氧化活性研究[D].硕士学位论文.长沙:中南林业科技大学,2014.

[10] 冯仰廉,周建民,张晓明,等.我国奶牛饲料产奶净能值测算方法的研究[J].中国畜牧杂志,1987(1):8-11.

[11] 郭玉新.王不留行提取物对泌乳中期奶牛生产性能和血液指标的影响[D].硕士学位论文.郑州:河南农业大学,2013.

[12] 高靖.葛根提取物对奶牛泌乳性能的影响及其机理研究[D].硕士学位论文.郑州:河南农业大学,2006.

[13] 贾若愚.竹提取物对泌乳后期奶牛生产性能和血液生化指标的影响[D].硕士学位论文.合肥:安徽农业大学,2011.

[14] 王放银.补充蛋氨酸可提高乳脂率[J].湖南饲料,2000(1):27.

[15] MCDONALD B,URRUTIA R,YIPP B G,et al.Intravascular neutrophil extracellular traps capture bacteria from the bloodstream during sepsis[J].Cell Host & Microbe,2012,12(3):324-333.

[16] 刘德义,顾方,王永鑫,等.大豆黄酮对奶牛血清抗氧化能力和免疫功能的影响[J].中国兽医学报,2008,28(11):1306-1309.

[17] 刘爱国,杨桂有,刘晓磊,等.牛血清中免疫球蛋白的提取研究[J].食品科学,2007,28(9):261-265.

[18] 朱志宁,郝振荣,王明,等.大豆异黄酮对高产奶牛泌乳后期乳腺肥大细胞分泌肿瘤坏死因子-α和表面型免疫球蛋白A水平的影响[J].动物营养学报,2011,23(1):112-121.

[19] YAGHOUBI S M J,GHORBANI G R,RAHMANI H R,et al.Growth,weaning performance and blood indicators of humoral immunity in Holstein calves fed supplemental flavonoids[J].Journal of Animal Physiology and Animal Nutrition,2008,92(4):456-462.

[20] ZHAN J S,LIU M M,SU X S,et al.Effects of alfalfa flavonoids on the production performance,immune system,and ruminal fermentation of dairy cows[J].Asian-Australasian Journal of Animal Sciences,2017,30(10):1416-1424.

[21] WOJTUNIK-KULESZA K A,ONISZCZUK A,ONISZCZUK T,et al.The influence of common free radicals and antioxidants on development of Alzheimer's disease[J].Biomedicine & Pharmacotherapy,2016,78:39-49.

[22] RAVISHANKAR D,RAJORA A K,GRECO F,et al.Flavonoids as prospective compounds for anti-cancer therapy[J].International Journal of Biochemistry & Cell Biology,2013,45(12):2821-2831.

[23] WINATA A,LORENZ K.Antioxidant potential of 5-n-pentadecylresorcinol[J].Journal of Food Processing and Preservation,1996,20(5):417-429.

[24] CUI J J,YUAN J F,ZHANG Z Q.Anti-oxidation activity of the crude polysaccharides isolated from Polygonum Cillinerve (Nakai) Ohwi in immunosuppressed mice[J].Journal of Ethnopharmacology,2010,132(2):512-517.

[25] 苏效双,占今舜,詹康,等.苜蓿黄酮对体外培养的奶牛乳腺上皮细胞增殖与抗氧化的影响[J].草业学报,2015,24(12):139-145.

[26] 杜仲叶抗疲劳功效分子机制与抗氧化作用关联性研究[D].博士学位论文.西安:陕西科技大学,2011.

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