3种生物饵料对宽体金线蛭幼蛭生长性能、消化酶活性及免疫力的影响

吴雷明, 韩光明, 寇祥明, 王守红, 张家宏, 毕建花, 王桂良, 唐鹤军, 徐荣, 朱凌宇

doi:10.3969/j.issn.1006-267x.2019.07.052

动物营养学报 >

2019 , Vol. 31 >Issue 7: 3383 - 3390

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.07.052

研究简报 Short Communications

3种生物饵料对宽体金线蛭幼蛭生长性能、消化酶活性及免疫力的影响

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1. 江苏里下河地区农业科学研究所, 扬州 225007;
2. 江苏省生态农业工程技术研究中心, 扬州 225009

吴雷明(1987-),男,山东临沂人,助理研究员,博士,研究方向为水产动物种质资源与种苗工程。E-mail:leiming4854@163.com

收稿日期: 2018-12-11

网络出版日期: 2019-07-19

基金资助

江苏省水产三项工程（2016-2018）；扬州市重点研发计划项目（YZ2018130）；江苏里下河地区农业科学研究所基金资助项目（2017-2020）

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Effects of Three Species of Live Food on Growth Performance, Digestive Enzyme Activities and Immunity of Whitmania pigra Larvae

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1. Agricultural Science Institute of Jiangsu Lixiahe District, Yangzhou 225007, China;
2. Research Center for Eco-Agricultural Engineering and Technology of Jiangsu Province, Yangzhou 225009, China

Received date: 2018-12-11

Online published: 2019-07-19

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摘要

本试验采用试验生态学方法，分析比较3种生物饵料对宽体金线蛭幼蛭生长性能、消化酶活性及免疫力的影响。将初始平均体质量为（0.027±0.010）g的宽体金线蛭幼蛭随机分为3组，每组设置3个重复，每个重复放置60条幼蛭。3组幼蛭分别以螺蛳（作为对照）、漂螺、蚯蚓为生物饵料。结果显示：漂螺组幼蛭体质量、体长及体宽3项生长指标均显著高于其他2组（P<0.05），3组之间幼蛭成活率无显著差异（P>0.05），整体生长性能由高至低依次为漂螺组、螺蛳组、蚯蚓组。幼蛭体质量与体长、体宽及体积的相关性由强到弱依次为体积（R²=0.912 0）、体长（R²=0.888 8）、体宽（R²=0.771 7）。幼蛭蛋白酶活性3组之间无显著差异（P>0.05），螺蛳组幼蛭淀粉酶活性显著高于漂螺组和蚯蚓组（P<0.05），漂螺组幼蛭脂肪酶活性显著高于螺蛳组和蚯蚓组（P<0.05）。幼蛭超氧化物歧化酶（SOD）活性和总抗氧化能力（T-AOC）3组之间无显著差异（P>0.05）。螺蛳组幼蛭碱性磷酸酶（AKP）、酸性磷酸酶（ACP）活性显著高于漂螺组和蚯蚓组（P<0.05）。蚯蚓组幼蛭过氧化氢酶（CAT）、溶菌酶（LZM）活性显著高于螺蛳组和漂螺组（P<0.05），螺蛳组和漂螺组之间无显著差异（P>0.05）。综合分析得出漂螺对宽体金线蛭幼蛭的促生长作用最佳，并建议依据漂螺营养成分制定幼蛭饲料营养标准，同时可将蚯蚓作为饲料免疫添加剂成分。

关键词： 宽体金线蛭; 生物饵料; 生长性能; 消化酶; 免疫力

本文引用格式

吴雷明, 韩光明, 寇祥明, 王守红, 张家宏, 毕建花, 王桂良, 唐鹤军, 徐荣, 朱凌宇 . 3种生物饵料对宽体金线蛭幼蛭生长性能、消化酶活性及免疫力的影响[J]. 动物营养学报, 2019 , 31(7) : 3383 -3390 . DOI: 10.3969/j.issn.1006-267x.2019.07.052

Abstract

The effects of three species of live food on the growth performance, digestive enzyme activities and immunity of Whitmania pigra larvae were analyzed by experimental ecology method in this experiment. A total of 540 Whitmania pigra larvae with an average body weight of (0.027±0.010) g were randomly divided into 3 groups with 3 replicates in each group and 60 larvae in each replicate. The snail (control), screw and earthworm were used as live food. The results showed that the body weight, body length and body width of larvae in screw group were significantly higher than those in the other two groups (P<0.05). There was no significant difference in survival rate among three groups (P>0.05). The overall growth performance of larvae from high to low was screw group, snail group and earthworm group. The correlation between growth indexes and body weight of larvae from large to small was volume (R²=0.912 0), body length (R²=0.888 8) and body width (R²=0.771 7). There was no significant difference in protease activity among snail group, screw group and earthworm group (P>0.05). The amylase activity of larval leech in snail group was significantly higher than that in screw group and earthworm group (P<0.05). The lipase activity of larval leech in screw group was significantly higher than that in snail group and earthworm group (P<0.05). There was no significant difference in superoxide dismutase (SOD) activity and total antioxidant capacity (T-AOC) among snail group, screw group and earthworm group (P>0.05). The alkaline phosphatase (AKP) and acid phosphatase (ACP) activities of larval leech in snail group were significantly higher than those in screw group and earthworm group (P<0.05). The catalase (CAT) and lysozyme (LZM) activities of larval leech in earthworm group were significantly higher than those in snail group and screw group (P<0.05), and there was no significant difference between snail group and screw group (P>0.05). Considered the growth and digestive enzyme activities, the screw is the best live food which can make leech larval acquire the best growth ability. Comprehensive analysis, the growth-promoting effect of screw is best for Whitmania pigra larvae. It is suggested that the nutritive standard of artificial feed for larval leech can be established according to the nutritive composition of screw, and earthworm can be used as the immune additive of artificial feed.

Key words： Whitmania pigra; live food; growth performance; digestive enzyme; immunity

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