研究简报 Short Communications

初生重对新生梅山仔猪肝脏发育和脂代谢的影响

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  • 扬州大学动物科学与技术学院, 扬州 225009
仲召鑫(1995-),男,江苏兴化人,硕士研究生,从事猪的营养与生理调控研究。E-mail:295764241@qq.com

收稿日期: 2019-01-06

  网络出版日期: 2019-07-19

基金资助

扬州大学镇江高新技术研究院开放课题(02);江苏省自然科学基金青年基金项目(BK20170490);江苏高校优势学科建设工程资助项目(PAPD)

Effects of Birth Weight on Liver Development and Lipid Metabolism of Meishan Newborn Piglets

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  • College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2019-01-06

  Online published: 2019-07-19

摘要

本试验旨在研究初生重对新生梅山仔猪肝脏发育和脂代谢的影响。选取正常初生重(NBW)和低初生重(LBW)新生梅山仔猪各6头,不喂食母乳,出生6 h内颈动脉放血致死。取仔猪血液测定血浆脂代谢指标,屠宰取样测定肝脏器官指数、肝脏脂代谢指标及相关基因的表达。结果表明:与NBW组相比,LBW组新生梅山仔猪的肝脏细胞粒径和面积极显著降低(P<0.01);血浆总胆固醇(TC)含量极显著升高(P<0.01),血浆低密度脂蛋白(LDL)含量显著升高(P<0.05),血浆甘油三酯(TG)含量极显著降低(P<0.01);肝脏TC含量显著降低(P<0.05),肝脏TG含量和脂蛋白脂酶(LPL)活性极显著降低(P<0.01),肝脏肝脂酶(HL)活性呈下降趋势(P=0.086);肝脏脂肪酸合成酶(FAS)和LPL的基因表达量极显著降低(P<0.01),肝脏激素敏感脂肪酶(HSL)的基因表达量显著降低(P<0.05)。上述结果显示,LBW会抑制新生梅山仔猪的肝脏发育,降低脂代谢功能。

本文引用格式

仲召鑫, 董丽, 毛俊舟, 张永胜, 喻礼怀 . 初生重对新生梅山仔猪肝脏发育和脂代谢的影响[J]. 动物营养学报, 2019 , 31(7) : 3391 -3397 . DOI: 10.3969/j.issn.1006-267x.2019.07.053

Abstract

The study aimed to determine the effects of birth weight on liver development and lipid metabolism of Meishan newborn piglets. Six normal birth weight (NBW) and six low birth weight (LBW) Meishan newborn piglets were selected without breast milk feeding and killed by jugular artery bloodletting within 6 hours of birth. Blood of piglets were taken to determine plasma lipid metabolism indexes, liver organ index, liver lipid metabolism indexes and related genes expression were measured after killed and sampled. The results showed as follows:compared with NBW group, liver cell diameter and area of Meishan newborn piglets in LBW group were significantly decreased (P<0.01); the content of total cholesterol (TC) in plasma was significantly increased (P<0.01), the content of low density lipoprotein (LDL) in plasma was significantly increased (P<0.05), and the content of triglyceride (TG) in plasma was significantly decreased (P<0.01); the content of TC in liver was significantly decreased (P<0.05), the content of TG and the activity of lipoprotein lipase (LPL) in liver were significantly decreased (P<0.01), and the activity of hepatic lipase (HL) in liver showed a downward trend (P=0.086); the gene expression levels of fatty acid synthase (FAS) and LPL in liver were significantly decreased (P<0.01), and the gene expression level of hormone-sensitive lipase (HSL) in liver was significantly decreased (P<0.05). In conclusion, LBW can inhibit liver improvement and decrease lipid metabolism function of Meishan newborn piglets.

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