动物营养学报 >

2019 , Vol. 31 >Issue 7: 3421 - 3430

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.07.056

研究简报 Short Communications

醋酸棉酚对绵羊瘤胃微生物数量及消化代谢的影响

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  • 1. 新疆农业大学动物科学学院, 新疆肉乳用草食动物营养重点实验室, 乌鲁木齐 830052;
    2. 中国农业大学动物科技学院, 动物营养学国家重点实验室, 北京 100193
李钰琪(1992-),女,新疆塔城人,硕士研究生,研究方向为草食动物营养代谢。E-mail:1476229635@qq.com

收稿日期: 2018-12-21

  网络出版日期: 2019-07-19

基金资助

中国农业大学-新疆农业大学联合基金项目（2017TC002）

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Effects of Gossypol Acetate on Rumen Microbial Population and Digestion and Metabolism in Sheep

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  • 1. Xinjiang Key Laboratory of Meat & Milk Production Herbivore Nutrition, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China;
    2. State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, China Agricultural University, Beijing 100193, China

Received date: 2018-12-21

  Online published: 2019-07-19

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摘要

本试验旨在研究添加醋酸棉酚后绵羊瘤胃微生物数量、瘤胃液代谢指标、水解酶活性的变化，探究醋酸棉酚对绵羊瘤胃微生物数量及消化代谢的影响。试验选用8只3岁左右、平均体重为（49.13±4.70）kg的健康哈萨克羊，安装永久性瘤胃瘘管后随机分为2组（每组4只），分别为对照组和试验组，试验分为2期，每期25 d。所有羊只饲喂同一营养水平粉状精料，每只羊每天粉状精料饲喂量为体重的1.50%，小麦秸秆及苜蓿为体重的1.00%，玉米青贮为体重的0.05%（干物质基础），混合饲喂，自由饮水；在此基础上，试验Ⅰ期试验组饲粮每天每只添加600 mg醋酸棉酚，试验Ⅱ期饲粮每天每只添加1 200 mg醋酸棉酚。结果表明：添加醋酸棉酚对绵羊瘤胃液pH及细菌总数量无显著影响（P>0.05），饲喂前（即0 h）试验组绵羊瘤胃液氨态氮浓度显著高于对照组（P<0.05），饲喂后6.0、9.0、12.0 h，试验组绵羊瘤胃液挥发性脂肪酸浓度显著低于对照组（P<0.05），试验Ⅰ期各时间点试验组绵羊瘤胃原虫总数量极显著低于对照组（P<0.01），饲喂后3.0 h，试验组绵羊瘤胃液纤维素酶活性显著高于对照组（P<0.05），饲喂后9.0、12.0 h，试验组绵羊瘤胃液蛋白酶活性极显著低于对照组（P<0.01）；试验Ⅱ期，饲喂后6.0 h，试验组绵羊瘤胃液蛋白酶活性显著低于对照组（P<0.05），饲喂后12.0 h极显著低于对照组（P<0.01）；添加醋酸棉酚后瘤胃液中棉酚的浓度在饲喂后1.5、3.0 h最高。综上，醋酸棉酚在初期摄入后显著降低绵羊瘤胃原虫数量；醋酸棉酚通过抑制绵羊瘤胃液蛋白酶活性，降低瘤胃液中戊酸、异戊酸及异丁酸的浓度而对瘤胃内蛋白质消化代谢产生影响，这种作用主要发生在摄入醋酸棉酚6.0 h后；醋酸棉酚对绵羊瘤胃液中的纤维素酶、淀粉酶的活性整体没有显著影响。

关键词： 绵羊; 醋酸棉酚; 瘤胃微生物; 瘤胃消化代谢

本文引用格式

李钰琪, 王彩蝶, 屯妮萨·麦提赛伊迪, 王世昌, 杨红建, 杨开伦 . 醋酸棉酚对绵羊瘤胃微生物数量及消化代谢的影响[J]. 动物营养学报, 2019 , 31(7) : 3421 -3430 . DOI: 10.3969/j.issn.1006-267x.2019.07.056

Abstract

This experiment aimed to explore the effects of gossypol acetate on rumen microbial population, digestion and metabolism in sheep by analyzing microbial population, rumen fluid metabolism indexes, and activity of some hydrolases in rumen after adding gossypol acetate. Eight Kazakh sheep aged about 3-years-old, with permanent rumen fistula, and an average body weight of (49.13±4.70) kg, were randomly divided into two groups (control group and trial group, four animals in each group). The experiment was divided into two phases, with 25 d in each phase. All sheep were fed the same powder concentrate, the feeding account of powder concentrate was 1.50% of body weight per sheep per day, wheat straw and alfalfa were 1.00% of body weight, corn silage was 0.05% of body weight (dry matter basis), and mixed feeding and free drinking water. On the basis of this diet, trial group in period Ⅰ and period Ⅱ added gossypol acetate (600 mg per sheep per day and 1 200 mg per sheep per day, respectively). The results showed that there was no significant difference in the rumen pH after adding gossypol acetate (P>0.05). The rumen ammonia nitrogen concentration in the trial group was significantly higher than that in the control group before feeding gossypol acetate (0 h) (P<0.05). The concentration of volatile fatty acid in the trial group was significantly lower than that in the control group at 6.0, 9.0 and 12.0 h after feeding (P<0.05). During the period Ⅰ, the total population of rumen fluid protozoa in the trial group was extremely significantly lower than that in the control group (P<0.01). The rumen cellulase activity in the trial group was significantly higher than that in the control group at 3.0 h after feeding (P<0.05). The protease activity was extremely significantly lower than that in the control group at 9.0 and 12.0 h after feeding (P<0.01). During the period Ⅱ, the protease activity in the trial group was significantly lower than that in the control group at 6.0 h after feeding (P<0.05), and extremely significantly lower than that in the control group at 12.0 h after feeding (P<0.01). After adding gossypol acetate, the gossypol concentration in rumen was the highest at 1.5 and 3.0 h after feeding. Therefore, the rumen protozoa population of sheep significantly reduces after initial intake of gossypol acetate. Gossypol acetate affects protein digestion and metabolism in rumen by inhibiting the protease activity, and reducing the concentration of valeric acid, isovaleric acid and isobutyric acid in rumen fluid. This effect mainly occurs at 6.0 h after intaking gossypol acetate. Gossypol acetate does not have significant effects on rumen fluid cellulase and amylase activities in sheep as a whole.

Key words： sheep; gossypol acetate; rumen microbial; rumen digestion and metabolism

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