水产营养 Aquaculture Nutrition

大菱鲆幼鱼对饲料中肌醇需求量的研究

  • 王雅平 ,
  • 李宝山 ,
  • 王际英 ,
  • 王成强 ,
  • 王晓艳 ,
  • 马长兴 ,
  • 王世信 ,
  • 孙永智 ,
  • 郝甜甜
展开
  • 1. 山东省海洋资源与环境研究院, 山东省海洋生态修复重点实验室, 烟台 264006;
    2. 上海海洋大学, 水产科学国家级实验教学示范中心, 农业部鱼类营养与环境生态研究中心, 水产动物遗传育种中心上海市协同创新中心, 上海 201306
王雅平(1992-),女,山东烟台人,硕士研究生,研究方向为水产动物营养与饲料。E-mail:m15552437686@163.com

收稿日期: 2019-05-08

  网络出版日期: 2019-11-19

基金资助

山东省重点研发计划项目(2016GSF115005);烟台市科技计划项目(2018ZHGY066)

Dietary Myo-Inositol Requirement of Juvenile Turbot (Scophthalmus maximus L.)

  • WANG Yaping ,
  • LI Baoshan ,
  • WANG Jiying ,
  • WANG Chengqiang ,
  • WANG Xiaoyan ,
  • MA Changxing ,
  • WANG Shixin ,
  • SUN Yongzhi ,
  • HAO Tiantian
Expand
  • 1. Shandong Key Laboratory of Marine Ecological Restoration, Shandong Marine Resource and Environment Research Institute, Yantai 264006, China;
    2. National Demonstration Center for Experimental Fisheries Science Education, Centre for Research on Environmental Ecology and Fish Nutrition(CREEFN) of the Ministry of Agriculture, Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breedin, Shanghai Ocean University, Shanghai 201306, China

Received date: 2019-05-08

  Online published: 2019-11-19

摘要

本试验旨在研究大菱鲆幼鱼对饲料中肌醇的需求量。以初始体重为(24.73±0.10)g的大菱鲆幼鱼为研究对象,在基础饲料中添加不同水平的肌醇,制成6种肌醇水平分别为319.00、511.00、703.00、1 087.00、1 855.00、3 391.00 mg/kg的等氮等能试验饲料,命名为M1~M6,在循环水养殖系统中进行80 d的养殖试验。结果显示:1)M2~M6组增重率(WGR)和特定生长率(SGR)显著高于M1组(P<0.05),M3~M6组饲料效率(FER)显著高于M1组(P<0.05)。2)M4~M6组全鱼粗脂肪含量显著低于其他3组(P<0.05),添加肌醇显著提高了肌肉粗脂肪含量(P<0.05),显著降低了肝脏粗脂肪含量(P<0.05)。3)M4组肠道脂肪酶活性显著高于其他各组(P<0.05)。4)随饲料肌醇水平的提高,血清超氧化物歧化酶(SOD)活性呈先升后降趋势,M3~M6组血清丙二醛(MDA)含量显著下降(P<0.05)。5)M3、M4组血清总胆固醇(TCHO)和甘油三酯(TG)含量均显著高于M1组(P<0.05)。6)M4组肝脏饱和脂肪酸(SFA)含量显著高于其他各组(P<0.05),肌肉和肝脏多不饱和脂肪酸(PUFA)含量分别在M4和M6组出现最大值。综上,饲料中添加适量肌醇可以提高大菱鲆幼鱼的生长性能、消化能力和抗氧化能力,调节血脂,改善脂肪酸组成。采用折线模型分析,以WGR和FER为评价指标,大菱鲆幼鱼对肌醇的需求量为622~991 mg/kg。

关键词: 大菱鲆; 肌醇; 生长; 需求

本文引用格式

王雅平 , 李宝山 , 王际英 , 王成强 , 王晓艳 , 马长兴 , 王世信 , 孙永智 , 郝甜甜 . 大菱鲆幼鱼对饲料中肌醇需求量的研究[J]. 动物营养学报, 2019 , 31(11) : 5122 -5132 . DOI: 10.3969/j.issn.1006-267x.2019.11.027

Abstract

This experiment was aimed to study the dietary myo-inositol (MI) requirement of juvenile turbot (Scophthalmus maximus L.). The juvenile turbot with initial body weight of (24.73±0.10) g were fed six isonitrogenous and isoenergergetic diets containing 319.00, 511.00, 703.00, 1 087.00, 1 855.00 and 3 391.00 mg/kg MI, named M1 to M6, respectively. The growth experiment lasted for 80 d. The results showed as follows:1) weight gain rate (WGR) and specific growth rate (SGR) in M2 to M6 groups were significantly higher than those in M1 group (P<0.05); while feed efficiency rate (FER) in M3 to M6 groups was significantly higher than that in M1 group (P<0.05). 2) The crude lipid content of the whole fish in M4 to M6 groups was significantly lower than that in the other groups (P<0.05), adding MI significantly increased the crude lipid content of the muscle (P<0.05), whereas significantly decreased the crude lipid content in the liver (P<0.05). 3) The intestinal lipase activity in M4 group was significantly higher than that in other groups (P<0.05). 4) With the increasing of dietary MI level, the serum superoxide dismutase (SOD) activity firstly deceased and then increased, and the serum malonaldehyde (MDA) content in M3 to M6 groups significantly decreased (P<0.05). 5) The serum total cholesterol (TCHO) and triglyceride (TG) contents in M3 and M4 groups were significantly higher than those in M1 group (P<0.05). 6) The saturated fatty acids (SFA) content in the liver was significantly higher than that in other groups (P<0.05), the polyunsaturated fatty acids (PUFA) content in liver and muscle increased significantly (P<0.05), and the maximum appeared in M6 and M4 groups, respectively. The findings suggest that the addition of proper level of MI in feed can improve the growth performance, digestion and antioxidant capacity of juvenile turbot, regulate serum lipid level, and improve fatty acid composition. Based on the broken-line regression analysis of WGR and FER, the dietary MI requirement of juvenile turbot is 622 to 991 mg/kg.

参考文献

[1] 张美彦,杨星,王常安,等.水产动物对肌醇需要量的研究[J].水产学杂志,2015,28(1):59-63.
[2] GONG W,LEI W,ZHU X,et al.Dietary myo-inositol requirement for juvenile gibel carp (Carassius auratus gibelio)[J].Aquaculture Nutrition,2014,20(5):514-519.  
[3] ZAKI M I,HELAL A M,FARRAG F H,et al.Impact of different levels of dietary myo-inositol on the growth performance,histological structure of gonads and liver of red tilapia reared in brackish water[J].African Journal of Biotechnology,2010,9(30):4808-4817.
[4] SHIAU S Y,SU S L.Dietary inositol requirement for juvenile grass shrimp,Penaeus monodon[J].Aquaculture,2004,241(1/2/3/4):1-8.
[5] KHOSRAVI S,LIM S J,RAHIMNEJAD S,et al.Dietary myo-inositol requirement of parrot fish,Oplegnathus fasciatus[J].Aquaculture,2015,436:1-7.
[6] 南韦肖,娄玉杰,司华哲.肌醇在动物生产中的应用[J].中国饲料,2014(19):7-8.
[7] BURTLE G J,LOVELL R T.Lack of response of channel catfish (Ictalurus punctatus) to dietary myo-inositol[J].Canadian Journal of Fisheries and Aquatic Sciences,1989,46(2):218-222.  
[8] BROWN M L,JARAMILLO F,Jr,GATLIN Ⅲ D M.Dietary phosphorus requirement of juvenile sunshine bass,Morone chrysops ♀×M. saxatilis[J].Aquaculture,1993,113(4):355-363.  
[9] AOE H,MASUDA I,MIMURA T,et al.Water-soluble vitamin requirements of carp.6.Requirement for thiamine and effects of antithiamines[J].Bulletin of the Japanese Society for the Science of Fish,1969,35:459-465.
[10] LEE B J,LEE K J,LIM S J,et al.Dietary myo-inositol requirement for olive flounder,Paralichthys olivaceus (Temminch et Schlegel)[J].Aquaculture Research,2008,40(1):83-90.  
[11] SHIAU S Y,SU S L.Juvenile tilapia (Oreochromis niloticus×Oreochromis aureus) requires dietary myo-inositol for maximal growth[J].Aquaculture,2005,243(1/2/3/4):273-277.
[12] DIAO S Q,HUANG Z,CHEN S S,et al.Effect of dietary inositol on growth,feed utilization and blood biochemical parameters for juvenile barramundi (Lates calcarifer Bloch)[J].American Journal of Agricultural and Biological Sciences,2010,5(3):370-375.  
[13] 程镇燕.大黄鱼和鲈鱼对几种水溶性维生素营养需求及糖类营养生理的研究[D].博士学位论文.青岛:中国海洋大学,2010.
[14] National Research Council.Nutrient requirements of fish[S].Washington,D.C.:National Academy Press,1993.
[15] GU M,BAI N,KORTNER T M.Taurocholate supplementation attenuates the changes in growth performance,feed utilization,lipid digestion,liver abnormality and sterol metabolism in turbot (Scophthalmus maximus) fed high level of plant protein[J].Aquaculture,2017,468:597-604.
[16] 王际英,李培玉,宋志东,等.饲料中添加丝兰提取物对大菱鲆幼鱼生长和生理及水环境的影响[J].水生生物学报,2014,38(6):1117-1126.
[17] 梅琳,周慧慧,麦康森,等.蛹肽蛋白替代鱼粉对大菱鲆(Scophthalmus maximus L.)幼鱼生长、饲料利用、消化代谢酶及免疫性能的影响[J].渔业科学进展,2015,36(3):85-92.
[18] BOONYARATPALIN M,WANAKOWAT J.Effect of thiamine,riboflavin,pantothenic acid and inositol on growth,feed efficiency and mortality of juvenile seabass[C]//Poster paper presented at the Ⅳ International Symposium on Fish Nutrition and Feeding.Biarritz,France:[s.n.],1991.
[19] JIANG W D,FENG L,LIU Y,et al.Growth,digestive capacity and intestinal microflora of juvenile Jian carp (Cyprinus carpio var. Jian) fed graded levels of dietary inositol[J].Aquaculture Research,2009,40(8):955-962.  
[20] 陈四清,季文娟,吕用琦,等.肌醇对黑鲷幼鱼营养作用的研究[J].海洋科学,1999(5):13-15.
[21] 田娟,文华,郜卫华,等.肌醇对大规格吉富罗非鱼生长、体组成和血清指标的影响[J].南方水产科学,2018,14(2):83-89.
[22] 蒋明,张志强,文华,等.肌醇对胭脂鱼生长、体成分和部分血清生化指标的影响[J].淡水渔业,2017,47(4):63-68.
[23] 冯琳,李江,周小秋,等.肌醇缺乏对幼建鲤消化功能和免疫功能的影响[J].动物营养学报,2009,21(6):878-883.
[24] SANDNES W,ROEM L.Effects of inositol supplementation on growth,chemical composition and blood chemistry in Atlantic salmon,Salmo salar L.,fry[J].Aquaculture Nutrition,1998,4(1):53-59.  
[25] DENG D F,HEMRE G I,WILSON R P.Juvenile sunshine bass (Morone chrysops♀×Morone saxatilis) do not require dietary myo-inositol[J].Aquaculture,2002,213(1/2/3/4):387-393.
[26] 姜维丹.肌醇对幼建鲤消化吸收能力和免疫能力的影响[D].硕士学位论文.雅安:四川农业大学,2008:57-70.
[27] ORUÇ E Ö,USTA D.Evaluation of oxidative stress responses and neurotoxicity potential of diazinon in different tissues of Cyprinus carpio[J].Environmental Toxicology and Pharmacology,2007,23(1):48-55.  
[28] 张美彦,王常安,徐奇友.肌醇对哲罗鲑生长性能、体成分及消化酶活性的影响[J].中国水产科学,2014,21(3):560-566.
[29] FERRO D,FRANCHI N,MANGANO V,et al.Characterization and metal-induced gene transcription of two new copper zinc superoxide dismutase in the solitary ascidian Ciona intestinalis[J].Aquatic Toxicology,2013,140-141:369-379.
[30] MAI K S,WU G T,ZHU W.Abalone,Haliotis discus hannai Ino,can synthesize myo-inositol de novo to meet physiological needs[J].The Journal of Nutrition,2001,131(11):2898-2903.  
[31] GLISZCZYN SKA-SWIGLO A.Antioxidant activity of water soluble vitamins in the TEAC (trolox equivalent antioxidant capacity) and the FRAP (ferric reducing antioxidant power) assays[J].Food Chemistry,2006,96(1):131-136.  
[32] DAVID M,MUNASWAMY V,HALAPPA R,et al.Impact of sodium cyanide on catalase activity in the freshwater exotic carp,Cyprinus carpio (Linnaeus)[J].Pesticide Biochemistry and Physiology,2008,92(1):15-18.  
[33] 吴宏玉,唐瞻杨,杨鸿昆,等.肌醇对奥尼罗非鱼生长、肝脏和肌肉脂肪含量及血清生化指标的影响[J].南方农业学报,2011,42(11):1415-1419.
[34] 文华,赵智勇,蒋明,等.草鱼幼鱼肌醇营养需要量的研究[J].中国水产科学,2007,14(5):794-800.
[35] 曹俊明,林鼎,薛华,等.四种抗脂肪肝物质降低草鱼肝胰脏脂质积累的替代关系[J].水生生物学报,1999,23(2):102-111.
[36] 柴广军.脂肪肝与血脂、转氨酶的影响关系分析[J].标记免疫分析与临床,2011,18(5):346-348.
[37] RODRÍGUEZ C,PÉREZ J A,HENDERSON R J.The esterification and modification of n-3 and n-6 polyunsaturated fatty acids by hepatocytes and liver microsomes of turbot (Scophthalmus maximus)[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,2002,132(3):559-570.  
[38] FARKAS T,FODOR E,KITAJKA K,et al.Response of fish membranes to environmental temperature[J].Aquaculture Research,2001,32(8):645-655.  
[39] 崔红红,刘波,戈贤平,等.肌醇对团头鲂幼鱼生长、血清生化及组织成分含量的影响[J].上海海洋大学学报,2013,22(6):868-875.
文章导航

/