动物营养学报 >

2019 , Vol. 31 >Issue 11: 5133 - 5141

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.11.028

水产营养 Aquaculture Nutrition

磷脂对日本沼虾虾青素沉积和利用的影响

  • 崔广同 ,
  • 邱小亮 ,
  • 任胜杰 ,
  • 倪沁 ,
  • 王倩倩 ,
  • 丁惠明 ,
  • 张铖 ,
  • 蔡春芳
展开
  • 1. 苏州大学基础医学与生物科学学院, 江苏省水产动物与营养重点实验室, 苏州 215123;
    2. 苏州市阳澄湖国家现代农业示范区发展有限公司, 苏州 215123
崔广同(1993-),男,安徽蚌埠人,硕士研究生,研究方向为水生生物学。E-mail:1328876491@qq.com

收稿日期: 2019-04-27

  网络出版日期: 2019-11-19

基金资助

江苏省农业科技自主创新资金项目[CX（15）1012-6]

收起

Effects of Phospholipid on Astaxanthin Deposition and Utilization of Macrobrachium nipponense

  • CUI Guangtong ,
  • QIU Xiaoliang ,
  • REN Shengjie ,
  • NI Qin ,
  • WANG Qianqian ,
  • DING Huiming ,
  • ZHANG Cheng ,
  • CAI Chunfang
Expand
  • 1. Key Laboratory of Aquatic Nutrition of Jiangsu Province, School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, China;
    2. Suzhou Yangchenghu National Modern Agricultural Industrial Park Co., Ltd., Suzhou 215123, China

Received date: 2019-04-27

  Online published: 2019-11-19

Fold

摘要

本研究旨在探讨磷脂对日本沼虾（Macrobrachium nipponense）虾青素沉积和利用的影响。磷脂水平分别设为0、20和40 g/kg，虾青素水平分别设为0和4 g/kg，根据饲料中磷脂和虾青素的添加水平设计6个组：不添加磷脂和虾青素的对照组（CON组），添加20和40 g/kg磷脂组（PL1和PL2组），添加4 g/kg虾青素组（AX组），添加20 g/kg磷脂+4 g/kg虾青素和40 g/kg磷脂+4 g/kg虾青素组（AXPL1和AXPL2组）。选择2 160尾平均体重为（1.00±0.00）g的日本沼虾，随机分为6组，每组3个重复，每个重复120尾。饲养4周后，每重复随机取20尾置于氨浓度为（32.25±0.42）mg/L的水体进行胁迫试验，分别于氨胁迫前及氨胁迫24 h后采样分析。结果表明：氨胁迫后，AX、AXLP1和AXLP2组的肝胰腺中虾青素含量显著高于CON组（P<0.05），且AXLP2组显著高于AX组（P<0.05）。三因子方差分析结果显示，饲料添加磷脂使肝胰腺虾青素含量、总超氧化物歧化酶（T-SOD）活性和总抗氧化能力（T-AOC）显著升高（P<0.05）；饲料添加虾青素使肝胰腺虾青素含量显著升高（P<0.05），超氧化物歧化酶（SOD）mRNA表达显著下调（P<0.05），肝胰腺T-SOD活性和T-AOC下降（P>0.05）；氨胁迫则使肝胰腺中虾青素含量显著升高（P<0.05），T-SOD活性和T-AOC显著下降（P<0.05）。与CON组相比，PL1、PL2、AX、AXPL1和AXPL2组的肝胰腺B细胞数量均相对增多。上述结果提示，磷脂有利于日本沼虾肝胰腺对外源虾青素的沉积和对内源虾青素的调用，体内沉积的虾青素对降低氧化应激损伤具有重要作用。

关键词： 日本沼虾; 磷脂; 虾青素; 抗氧化; B细胞

本文引用格式

崔广同 , 邱小亮 , 任胜杰 , 倪沁 , 王倩倩 , 丁惠明 , 张铖 , 蔡春芳 . 磷脂对日本沼虾虾青素沉积和利用的影响[J]. 动物营养学报, 2019 , 31(11) : 5133 -5141 . DOI: 10.3969/j.issn.1006-267x.2019.11.028

Abstract

The aim of this study was to evaluate the effects of phospholipid on astaxanthin deposition and utilization of Macrobrachium nipponense. The phospholipid levels were set at 0, 20 and 40 g/kg, respectively, and the astaxanthin levels were set at 0 and 4 g/kg, respectively. According to the supplemental level of phospholipid and astaxanthin, six groups were designed:the control group (CON group) without phospholipid and astaxanthin supplementation, supplemented 20 and 40 g/kg phospholipid groups (PL1 and PL2 groups), supplemented 4 g/kg astaxanthin group (AX group), supplemented 4 g/kg astaxanthin plus 20 g/kg phospholipid and 4 g/kg astaxanthin plus 40 g/kg phospholipid groups (AXPL1 and AXPL2 groups). A total of 2 160 Macrobrachium nipponense with initial body weight of (1.00±0.00) g were selected and randomly divided into 3 groups with 3 replicates per group and 120 prawns per replicate. After feeding 4 weeks, twenty prawns were randomly sampled from each replicate and exposed to (32.25±0.42)mg/L of ammonia concentration in a stress experiment, and sampling and analysis were conducted before and after 24 h of ammonia stress. The results showed that after ammonia stress, the hepatopancreas astaxanthin content of AX, AXLP1 and AXLP2 groups was significantly higher than that of the CON group (P<0.05), and AXLP2 group was significantly higher than AX group (P<0.05). Three-way analysis of variance results showed that dietary phospholipid significantly increased the astaxanthin content, total superoxide dismutase (T-SOD) activity and total antioxidant capacity (T-AOC) in hepatopancreas (P<0.05); dietary astaxanthin significantly increased the hepatopancreas astaxanthin content (P<0.05), significantly down-regulated the superoxide dismutase (SOD) mRNA relative expression (P<0.05), and significantly decreased the hepatopancreas T-AOC and T-SOD activity (P>0.05); while ammonia stress significantly increased the astaxanthin content (P<0.05), and significantly decreased the hepatopancreas T-AOC and T-SOD activity (P<0.05). Compared with the CON group, the hepatopancreas B cells number of PL1, PL2, AX, AXPL1 and AXPL2 groups was relative increased. These results suggest that phospholipids improve deposition of exogenous astaxanthin and facilitate utilization of endogenous astaxanthin by Macrobrachium nipponense, and that accumulated astaxanthin in vivo plays an important role in reducing oxidative injury.

Key words： Macrobrachium nipponense; phospholipid; astaxanthin; antioxidation; B cell

参考文献

[1] 齐霁,陈立侨,孙盛明,等.不同磷脂水平下中华绒螯蟹幼蟹的胆碱需要量[J].中国水产科学,2013,20(2):372-379.
[2] DALL W.Carotenoids versus retinoids (vitamin A) as essential growth factors in penaeid prawns (Penaeus semisulcatus)[J].Marine Biology,1995,124(2):209-213.  
[3] HUSSEIN G,GOTO H,ODA S,et al.Antihypertensive potential and mechanism of action of astaxanthin:Ⅲ.Antioxidant and histopathological effects in spontaneously hypertensive rats[J].Biological and Pharmaceutical Bulletin,2006,29(4):684-688.  
[4] HIGUERA-CIAPARA I,FÉLIX-VALENZUELA L,GOYCOOLEA F M.Astaxanthin:a review of its chemistry and applications[J].Critical Reviews in Food Science and Nutrition,2006,46(2):185-196.  
[5] YAMADA S,TANAKA Y,SAMESHIMA M,et al.Pigmentation of prawn (Penaeus japonicus) with carotenoids:Ⅰ.Effect of dietary astaxanthin,β-carotene and canthaxanthin on pigmentation[J].Aquaculture,1990,87(3/4):323-330.
[6] BAKER R T M,PFEIFFER A M,SCHÖNER F J,et al.Pigmenting efficacy of astaxanthin and canthaxanthin in fresh-water reared Atlantic salmon,Salmo salar[J].Animal Feed Science and Technology,2002,99(1/2/3/4):97-106.
[7] 胡玲,曾秋凤,丁雪梅,等.饲粮添加大豆卵磷脂乳化剂对肉鸡生长性能、养分利用率和血清生化指标的影响[J].动物营养学报,2012,24(10):1928-1938.
[8] CAI X H,GRANT D J W,WIEDMANN T S.Analysis of the solubilization of steroids by bile salt micelles[J].Journal of Pharmaceutical Sciences,1997,86(3):372-377.  
[9] CACECI T,NECK K F,LEWIS D D H,et al.Ultrastructure of the hepatopancreas of the Pacific white shrimp,Penaeus vannamei (Crustacea:Decapoda)[J].Journal of the Marine Biological Association of the United Kingdom,1988,68(2):323-337.  
[10] FENG L,CHEN Y P,JIANG W D,et al.Modulation of immune response,physical barrier and related signaling factors in the gills of juvenile grass carp (Ctenopharyngodon idella) fed supplemented diet with phospholipids[J].Fish & Shellfish Immunology,2016,48:79-93.
[11] 王志,崔广同,蔡春芳,等.磷脂影响日本沼虾对饲料中脂肪的需要量[J].动物营养学报,2018,30(6):222-231.
[12] 邹李昶,任夙艺,王志铮,等.氨氮急性胁迫对日本沼虾(Macrobrachium nipponensis)死亡率、耗氧率及窒息点的影响[J].海洋与湖沼,2015,46(1):206-211.
[13] WANG Z,CAI C F,CAO X M,et al.Supplementation of dietary astaxanthin alleviated oxidative damage induced by chronic high pH stress,and enhanced carapace astaxanthin concentration of Chinese mitten crab Eriocheir sinensis[J].Aquaculture,2018,483:230-237.
[14] 原居林.克氏原螯虾和日本沼虾体内微囊藻毒素累积净化规律研究[D].博士学位论文.咸阳:西北农林科技大学2016.
[15] LIVAK K J,SCHMITTGEN T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method[J].Methods,2001,25(4):402-408.  
[16] 麻楠,龙晓文,赵磊,等.饲料中添加合成虾青素对中华绒螯蟹成体雌蟹性腺发育、色泽和抗氧化能力的影响[J].水生生物学报,2017,41(4):755-765.
[17] STOREBAKKEN T,NO H K.Pigmentation of rainbow trout[J].Aquaculture,1992,100(1/2/3):209-229.
[18] BRITTON G.Structure and properties of carotenoids in relation to function[J].FASEB Journal,1995,9(15):1551-1558.  
[19] OLSEN R E,KIESSLING A,MILLEY J E,et al.Effect of lipid source and bile salts in diet of Atlantic salmon,Salmo salar L.,on astaxanthin blood levels[J].Aquaculture,2005,250(3/4):804-812.
[20] HOLDOM M D,LECHENNE B,HAY R J,et al.Production and characterization of recombinant Aspergillus fumigatus Cu,Zn superoxide dismutase and its recognition by immune human sera[J].Journal of Clinical Microbiology,2000,38(2):558-562.
[21] WANG W N,ZHOU J,WANG P,et al.Oxidative stress,DNA damage and antioxidant enzyme gene expression in the Pacific white shrimp,Litopenaeus vannamei when exposed to acute pH stress[J].Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology,2009,150(4):428-435.  
[22] 裴素蕊,管越强,马云婷.饲料中添加虾青素对凡纳滨对虾生长、存活和抗氧化能力的影响[J].水产科学,2009,28(3):126-129.
[23] WANG W N,WANG A L,ZHANG Y J.Effect of dietary higher level of selenium and nitrite concentration on the cellular defense response of Penaeus vannamei[J].Aquaculture,2006,256(1/2/3/4):558-563.
[24] XIE S W,FANG W P,WEI D,et al.Dietary supplementation of Haematococcus pluvialis improved the immune capacity and low salinity tolerance ability of post-larval white shrimp,Litopenaeus vannamei[J].Fish & Shellfish Immunology,2018,80:452-457.
[25] DUAN Y F,ZHANG Y,DONG H B,et al.Effect of desiccation on oxidative stress and antioxidant response of the black tiger shrimp Penaeus monodon[J].Fish & Shellfish Immunology,2016,58:10-17.
[26] GUNAWICKRAMA S H N P,AARSAETHER N,ORBEA A,et al.PCB77(3,3',4,4'-tetrachlorobiphenyl) co-exposure prolongs CYP1A induction,and sustains oxidative stress in B(a)P-exposed turbot,Scophthalmus maximus,in a long-term study[J].Aquatic Toxicology,2008,89(2):65-74.  
[27] 洪美玲,陈立侨,顾顺樟,等.氨氮胁迫对中华绒螯蟹免疫指标及肝胰腺组织结构的影响[J].中国水产科学,2007,14(3):412-418.
[28] ZHANG J,LIU Y J,TIAN L X,et al.Effects of dietary astaxanthin on growth,antioxidant capacity and gene expression in Pacific white shrimp Litopenaeus vannamei[J].Aquaculture Nutrition,2013,19(6):917-927.  
[29] 赵卫红,王资生,张余霞,等.雌二醇对日本沼虾肝胰腺的脂肪酸含量及组织结构的影响[J].海洋渔业,2014,36(6):542.
[30] PINHO G L L,DA ROSA C M,YUNES J S,et al.Toxic effects of microcystins in the hepatopancreas of the estuarine crab Chasmagnathus granulatus (Decapoda,Grapsidae)[J].Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology,2003,135(4):459-468.  
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2