动物营养学报 >

2019 , Vol. 31 >Issue 11: 5178 - 5183

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.11.033

实验动物 Laboratory animal

叶酸对铅暴露大鼠肝脏氧化损伤的影响

  • 李宁 ,
  • 曹帅 ,
  • 沈玥 ,
  • 张迪 ,
  • 宋莲军 ,
  • 黄现青 ,
  • 乔明武 ,
  • 张平安 ,
  • 王凡 ,
  • 赵紫薇 ,
  • 张凡
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  • 1. 河南农业大学食品科学技术学院, 郑州 450002;
    2. 河南省食品加工与流通安全控制工程技术研究中心, 郑州 450002
李宁(1981-),女,河南西平人,副教授,博士,主要从事食品营养与安全研究。E-mail:lining8028@126.com

收稿日期: 2019-04-03

  网络出版日期: 2019-11-19

基金资助

国家自然科学基金项目（31201878，U1604183）；河南省重点研发与推广专项（科技攻关）（182102310252）；河南农业大学大学生实践创新训练计划项目（201910466024）

收起

Effects of Folic Acid on Liver Oxidative Damage in Lead-Exposed Rats

  • LI Ning ,
  • CAO Shuai ,
  • SHEN Yue ,
  • ZHANG Di ,
  • SONG Lianjun ,
  • HUANG Xianqing ,
  • QIAO Mingwu ,
  • ZHANG Ping'an ,
  • WANG Fan ,
  • ZHAO Ziwei ,
  • ZHANG Fan
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  • 1. College of Food Science and Technology, Henan Agriculture University, Zhengzhou 450002, China;
    2. Henan Engineering and Technology Research Center of Safety Control of Food Processing and Circulation, Zhengzhou 450002, China

Received date: 2019-04-03

  Online published: 2019-11-19

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摘要

本试验旨在探讨叶酸对铅诱导的大鼠肝脏内氧化应激及核因子E2相关因子（Nrf2）、血红素氧合酶-1（HO-1）蛋白表达的影响。将40只2月龄雄性大鼠随机平均分为4组（n=10），进行如下处理：对照组，自由饮水+灌胃1 mL去离子水；染铅组，自由饮0.2%的醋酸铅溶液+灌胃1 mL去离子水；干预组，自由饮0.2%的醋酸铅溶液+灌胃1 mL叶酸悬浊液（按照0.4 mg/kg BW叶酸的剂量配制）；叶酸组，自由饮水+灌胃1 mL叶酸悬浊液（按照0.4 mg/kg BW叶酸的剂量配制）。各组大鼠每天灌胃1次，连续灌胃14 d后采用相应的试剂盒测定大鼠肝脏中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性与丙二醛（MDA）、还原型谷胱甘肽（GSH）含量，采用免疫组织化学法测定肝脏中Nrf2、HO-1蛋白的表达量。结果显示：与对照组相比，染铅组肝脏中GSH含量、GSH-Px活性显著降低（P<0.05），血清中铅含量以及肝脏中MDA含量与HO-1和Nrf2蛋白的表达量显著升高（P<0.05）。与染铅组相比，干预组肝脏中MDA含量以及Nrf2、HO-1蛋白的表达量显著下降（P<0.05）。与对照组相比，干预组血清中铅含量显著增加（P<0.05），肝脏中SOD活性、GSH与MDA含量及Nrf2和HO-1蛋白的表达量均无显著变化（P>0.05）。此外，叶酸组肝脏中GSH-Px活性与对照组相比显著上升（P<0.05）。由此得出，叶酸对铅暴露大鼠的肝脏氧化损伤有一定程度的保护作用。

关键词： 叶酸; 铅; 肝脏; 氧化活性; 核因子E2相关因子; 血红素氧合酶-1

本文引用格式

李宁 , 曹帅 , 沈玥 , 张迪 , 宋莲军 , 黄现青 , 乔明武 , 张平安 , 王凡 , 赵紫薇 , 张凡 . 叶酸对铅暴露大鼠肝脏氧化损伤的影响[J]. 动物营养学报, 2019 , 31(11) : 5178 -5183 . DOI: 10.3969/j.issn.1006-267x.2019.11.033

Abstract

The aim of this study was to investigate the effects of folic acid on lead-induced oxidative stress and nuclear factor E2-related factors (Nrf2) and heme oxygenase-1 (HO-1) protein expression in rat liver. Forty 2-month-old male rats were randomly divided into 4 groups (n=10) and treated as follows:control group, free drinking water+1 mL deionized water was intragastrically administered; lead group, free drink 0.2% lead acetate solution+1 mL deionized water was intragastrically administered; intervention group, free drink 0.2% lead acetate solution+1 mL folic acid suspension was intragastrically administered (prepared according to the dose of 0.4 mg/kg BW folic acid); folic acid group, free drinking water+1 mL folic acid suspension was intragastrically administered (prepared according to the dose of 0.4 mg/kg BW folic acid). Rats in each group were given intragastric administration once a day. After 14 days, the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and the contents of malondialdehyde (MDA) and reduced glutathione (GSH) in rat liver were determined by corresponding kits, and the expression levels of Nrf2 and HO-1 proteins in liver were determined by immunohistochemistry. The results showed that compared with the control group, the liver GSH content and GSH-Px activity in the lead-exposed group were significantly decreased (P<0.05), while the serum lead content and the MDA content and the expression levels of HO-1 and Nrf2 proteins in liver were significantly increased (P<0.05). Compared with the lead-exposed group, the MDA content and the expression levels of Nrf2 and HO-1 proteins in liver of the intervention group were significantly decreased (P<0.05). Compared with the control group, the serum lead content in the intervention group was significantly increased (P<0.05), and there were no significant changes in SOD activity, GSH and MDA contents and Nrf2 and HO-1 protein expression levels in liver (P>0.05).In addition, the activity of GSH-Px in liver of folic acid group was significantly higher than that of the control group (P<0.05). It is concluded that folic acid has a certain degree of protective effect on liver oxidative damage in lead-exposed rats.

Key words： folic acid; lead; liver; oxidative activity; Nrf2; HO-1

参考文献

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