动物营养学报 >

2019 , Vol. 31 >Issue 11: 5214 - 5220

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.11.037

分子营养 Molecular Nutrition

5-羟色胺前体物对围产期母羊肝脏糖异生关键基因mRNA和蛋白质表达量的影响

  • 桑丹 ,
  • 娜美日嘎 ,
  • 孙海洲 ,
  • 金鹿 ,
  • 张崇志 ,
  • 张春华 ,
  • 茹婷 ,
  • 贾春雷 ,
  • 敖长金
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  • 1. 内蒙古农业大学动物科学学院, 呼和浩特 010018;
    2. 内蒙古农牧业科学院动物营养与饲料研究所, 呼和浩特 010031;
    3. 乌拉特前旗家畜改良工作站, 巴彦淖尔 014400
桑丹(1978-),女,内蒙古呼和浩特人,硕士研究生,从事反刍动物营养研究。E-mail:sangdan1999@126.com

收稿日期: 2019-06-26

  网络出版日期: 2019-11-19

基金资助

内蒙古农牧业科技创新基金项目（2018CXJJM04）；国家绒毛用羊产业技术体系（CARS-39-11）；内蒙古自治区科技成果转化项目（CGZH2018166）；公益性行业（农业）科研专项（201303062）

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Effects of Serotonin Precursor on mRNA and Protein Expressions of Liver Gluconeogenesis Key Genes of Perinatal Ewes

  • SANG Dan ,
  • NAMIRGA ,
  • SUN Haizhou ,
  • JIN Lu ,
  • ZHANG Chongzhi ,
  • ZHANG Chunhua ,
  • RU Ting ,
  • JIA Chunlei ,
  • AO Changjin
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  • 1. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
    2. Animal Nutrition and Feed Research Institution of Inner Mongolia Academy of Agriculture and Animal Husbandry Academy, Hohhot 010031, China;
    3. Urat Front Banner Livestock Improvement Station, Bayannur 014400, China

Received date: 2019-06-26

  Online published: 2019-11-19

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摘要

本试验旨在研究5-羟色胺（5-HT）前体物对围产期母羊肝脏糖异生关键基因mRNA和蛋白质表达量的影响。试验选择体况良好、体重为（64.52±4.11）kg、处于同一围产期的30只巴美肉羊母羊，按体重随机分为3组，分别为对照组（灌注生理盐水）、5-羟基色胺酸（5-HTP）组（灌注5-HTP）和色氨酸（Trp）组（灌注Trp），每组10只。在母羊围产期产前第7天至产后当天（第0天）进行颈静脉灌注，5-HTP和Trp的灌注剂量均为0.178 mg/kg BW，浓度为0.1 mg/mL；对照组灌注同等剂量的生理盐水。试验期为产前第7天至产后第30天。结果表明：1）5-HTP组和Trp组的肝脏磷酸烯醇丙酮酸羧激酶（PEPCK）、葡萄糖-6-磷酸酶（G6P）和丙酮酸羧化酶（PC）的mRNA表达量均显著高于对照组（P<0.05）。2）5-HTP组和Trp组的肝脏PEPCK、PC和G6P的蛋白质表达量均显著高于对照组（P<0.05），Trp组的肝脏PEPCK的蛋白质表达量显著低于5-HTP组（P<0.05），Trp组的肝脏G6P的蛋白质表达量显著高于5-HTP组（P<0.05）。由此可见，5-HT前体物不仅在转录水平，也在翻译水平起到促进肝脏糖异生的作用。

关键词： 5-羟色胺前体物; 肝脏糖异生; 围产期; 母羊

本文引用格式

桑丹 , 娜美日嘎 , 孙海洲 , 金鹿 , 张崇志 , 张春华 , 茹婷 , 贾春雷 , 敖长金 . 5-羟色胺前体物对围产期母羊肝脏糖异生关键基因mRNA和蛋白质表达量的影响[J]. 动物营养学报, 2019 , 31(11) : 5214 -5220 . DOI: 10.3969/j.issn.1006-267x.2019.11.037

Abstract

This study was conducted to investigate the effects of serotonin (5-HT) precursor on mRNA and protein expressions of liver gluconeogenesis key genes of perinatal ewes. Thirty good body condition Bamei ewes with a body weight of (64.52±4.11) kg in the same perinatal period were randomly divided into 3 groups according to their body weight, which were control group, 5-hydroxy-tryptophan (5-HTP) group (perfused 5-HTP) and tryptophan (Trp) group (perfused Trp), respectively, each group contained 10 ewes. Ewes jugular vein perfusion was conducted from prenatal day 7 to postpartum day 0, the perfusion dose of 5-HTP and Trp was 0.178 mg/kg BW, and the concentration was 0.1 mg/mL; the control group was perfused with the same dose of saline. The experiment lasted form prenatal day 7 to postpartum day 30. The results showed as follows:1) the mRNA expressions of phosphoenolpyruvate carboxykinase (PEPCK), glucose-6-phosphatase (G6P) and pyruvate carboxylase (PC) in liver of 5-HTP group and Trp group were significantly higher than those of the control group (P<0.05). 2) The protein expressions of PEPCK, G6P and PC in liver of 5-HTP group and Trp group were significantly higher than those of the control group (P<0.05), the liver PEPCK protein expression of Trp group was significantly lower than that of 5-HTP group (P<0.05), and the liver G6P protein expression of Trp group was significantly higher than that of 5-HTP group (P<0.05). In conclusion, 5-HT precursor plays a role in promoting the liver gluconeogenesis not only at the transcriptional level, but also at the translation level.

Key words： serotonin precursor; liver gluconeogenesis; perinatal period; ewes

参考文献

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