饲料科学与技术 Feed science and technology

黑水虻幼虫粉替代鱼粉对凡纳滨对虾幼虾生长性能、体组成、血清生化指标和抗氧化能力的影响

  • 胡俊茹 ,
  • 王国霞 ,
  • 黄文庆 ,
  • 赵红霞 ,
  • 莫文艳 ,
  • 黄燕华
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  • 1. 广东省农业科学院动物科学研究所, 农业农村部华南动物营养与饲料重点实验室, 广东省动物育种与营养公共实验室, 广东省畜禽育种与营养研究重点实验室, 广州 510640;
    2. 广州飞禧特生物科技有限公司, 广州 510640
胡俊茹(1979-),女,河北唐山人,副研究员,博士,从事水产动物营养与饲料研究。E-mail:hujunru1025@163.com

收稿日期: 2019-04-27

  网络出版日期: 2019-11-19

基金资助

广东省现代农业科技创新联盟建设项目(2018LM1082,2018LM1083);广东省农业科学院学科团队建设项目(201614TD)

Effects of Fish Meal Replacement by Black Soldier Fly (Hermetia illucens) Larvae Meal on Growth Performance, Body Composition, Serum Biochemical Indexes and Antioxidant Ability of Juvenile Litopenaeus vannamei

  • HU Junru ,
  • WANG Guoxia ,
  • HUANG Wenqing ,
  • ZHAO Hongxia ,
  • MO Wenyan ,
  • HUANG Yanhua
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  • 1. Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangdong Public Laboratory of Animal Breeding and Nutrition, Key Laboratory of Animal Nutrition and Feed Science in South China of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
    2. Guangzhou Fishtech Biotechnology Co., Ltd., Guangzhou 510640, China

Received date: 2019-04-27

  Online published: 2019-11-19

摘要

在实用饲料(含20%鱼粉,鱼粉粗蛋白质含量为73.7%)的基础上,用黑水虻幼虫粉分别替代0(G0,作为对照)、10%(G10组)、15%(G15)、20%(G20)、25%(G25)、30%(G30)的鱼粉,配制成6种等氮等能的试验饲料,以研究黑水虻幼虫粉替代鱼粉对凡纳滨对虾幼虾生长性能、体组成、血清生化指标和抗氧化能力的影响。将720尾初始体重为1.80 g左右的凡纳滨对虾幼虾随机分成6组,每组设3个重复,每个重复40尾。试验为期8周。结果表明:G15组饲料系数显著低于G0组(P<0.05),各替代组(G10~G30组)幼虾的增重率、特定生长率和存活率与G0组相比有升高的趋势,但差异未达显著水平(P>0.05)。各组虾体的粗蛋白质、粗脂肪、总磷和水分含量差异不显著(P>0.05),G25组虾体的粗灰分和钙含量较G0组显著升高(P<0.05)。各组幼虾血清白蛋白、球蛋白、胆固醇、甘油三酯、葡萄糖、尿酸含量及谷丙转氨酶和谷草转氨酶活性无显著差异(P>0.05)。幼虾血清总抗氧化能力以及过氧化物酶、过氧化氢酶活性均在G20组达到最高,且G15~G30组血清过氧化物酶活性显著高于G0组(P<0.05),G15~G20组血清过氧化氢酶活性显著高于G0组(P<0.05);G25组血清超氧化物歧化酶活性显著高于G0组(P<0.05)。综上所述,黑水虻幼虫粉替代饲料中30%或以下的鱼粉对凡纳滨对虾幼虾的生长性能有一定的促进作用,替代水平为15%时生长性能最佳;黑水虻幼虫粉替代饲料中15%~20%的鱼粉可显著增强凡纳滨对虾幼虾血清中抗氧化酶的活性,进而提高机体抗氧化能力。

本文引用格式

胡俊茹 , 王国霞 , 黄文庆 , 赵红霞 , 莫文艳 , 黄燕华 . 黑水虻幼虫粉替代鱼粉对凡纳滨对虾幼虾生长性能、体组成、血清生化指标和抗氧化能力的影响[J]. 动物营养学报, 2019 , 31(11) : 5292 -5300 . DOI: 10.3969/j.issn.1006-267x.2019.11.046

Abstract

In order to study the effects of fish meal replacement by black soldier fly (Hermetia illucens) larvae meal on growth performance, body composition, serum biochemical indexes and antioxidant ability of juvenile Litopenaeus vannamei, six isonitrogenous and isoenergetic experimental diets were prepared by replacing 0 (G0), 10% (G10), 15% (G15), 20% (G20), 25% (G25) and 30% (G30) of fish meal with black soldier fly larvae meal on a practical diet (fish meal content was 20.0% and crude protein content in fish meal was 73.7%). A total of 720 juvenile Litopenaeus vannamei with an initial body weight about 1.80 g were randomly divided into 6 groups with 3 replicates per group and 40 shrimps per replicates. The experiment lasted for 8 weeks. The results showed that the feed coefficient in G15 group was significantly lower than that in G0 group (P<0.05), the weight gain rate, specific growth rate and survival rate in substitute groups (G10 to G30 groups) were higher than those in G0 group, but the differences were not significant (P>0.05). There were no significant differences in crude protein, ether extract, total phosphorus and moisture contents in whole body among groups (P>0.05), but the ash and calcium contents in G25 group were significantly increased compared with G0 group (P<0.05). No significant differences in serum albumin, globulin, cholesterol, triglyceride, glucose, uric acid contents and glutamate transaminase and glutamate transaminase activities were found in all groups (P>0.05). Total antioxidant capacity (T-AOC), peroxidase (POD) and catalase (CAT) activities in serum reached the highest levels in G20 group, the serum POD activity in G15 to G30 groups was significantly higher than that in G0 group (P<0.05), and the serum CAT activity in G15 to G20 groups was significantly higher than that in G0 group (P<0.05). Serum superoxide dismutase (SOD) activity in G25 group was significantly higher than that in G0 group (P<0.05). It is concluded that black soldier fly larvae meal can replace not more than 30% of the fish meal in the diet and promote the growth performance of juvenile Litopenaeus vannamei, the best growth performance is achieved when the substitution level is 15%. In addition, when the substitution level is 15% to 20%, the black soldier fly larvae meal can significantly increase the activities of antioxidant enzymes in serum and improve the antioxidant ability of juvenile Litopenaeus vannamei.

参考文献

[1] 安新城,吕欣.黑水虻的生物学特性及营养价值[J].养殖与饲料,2007(11):67-68.
[2] 胡俊茹,何飞,莫文艳,等.采食不同有机废弃物黑水虻幼虫饲料价值分析[J].中国饲料,2017(15):24-27.
[3] 喻国辉,陈燕红,喻子牛,等.黑水虻幼虫和预蛹的饲料价值研究进展[J].昆虫知识,2009,46(1):41-45.
[4] HENRY M,GASCO L,PICCOLO G,et al.Review on the use of insects in the diet of farmed fish:past and future[J].Animal Feed Science and Technology,2015,203:1-22.
[5] 吉红,李森林,徐歆歆.昆虫资源在水产饲料中的应用研究进展[J].饲料工业,2016,37(22):1-9.
[6] ST-HILAIRE S,SHEPPARD C,TOMBERLIN J K,et al.Fly prepupae as a feedstuff for rainbow trout,Oncorhynchus mykiss[J].Journal of the World Aquaculture Society,2007,38(1):59-67.  
[7] 胡俊茹,王国霞,莫文艳,等.黑水虻幼虫粉替代鱼粉对鲈鱼幼鱼生长性能、体组成、血浆生化指标和组织结构的影响[J].动物营养学报,2018,30(2):613-623.
[8] HU J R,WANG G X,HUANG Y H,et al.Effects of substitution of fish meal with black soldier fly (Hermetia illucens) larvae meal,in yellow catfish (Pelteobagrus fulvidraco) diets[J].The Israeli Journal of Aquaculture-Bamidgeh,2017,69:1382.
[9] ZHOU J S,LIU S S,JI H,et al.Effect of replacing dietary fish meal with black soldier fly larvae meal on growth and fatty acid composition of Jian carp (Cyprinus carpio var. Jian)[J].Aquaculture Nutrition,2017,24:424-433.
[10] 刘兴,孙学亮,李连星,等.黑水虻替代鱼粉对锦鲤生长和健康状况的影响[J].大连海洋大学学报,2017,32(4):422-427.
[11] KROECKEL S,HARJES A G E,ROTH I,et al.When a turbot catches a fly:evaluation of a pre-pupae meal of the black soldier fly (Hermetia illucens) as fish meal substitute-growth performance and chitin degradation in juvenile turbot (Psetta maxima)[J].Aquaculture,2012,364-365:345-352.
[12] 易昌金,胡俊茹,胡毅,等.凡纳滨对虾对黑水虻幼虫粉营养物质的表观消化率[J].饲料工业,2018,39(4):21-26.
[13] CUMMINS V C,RAWLES S D,THOMPSON K R,et al.Evaluation of black soldier fly (Hermetia illucens) larvae meal as partial or total replacement of marine fish meal in practical diets for Pacific white shrimp (Litopenaeus vannamei)[J].Aquaculture,2017,473:337-344.
[14] 曹俊明,严晶,黄燕华,等.家蝇蛆粉替代鱼粉对凡纳滨对虾生长、抗氧化和免疫指标的影响[J].水产学报,2012,36(4):529-537.
[15] 胡水城.脱脂蚕蛹粉替代鱼粉对凡纳滨对虾生长、肝胰腺组织结构和蜕壳周期的影响[D].硕士学位论文.厦门:集美大学,2017.
[16] WANG G X,PENG K,HU J R,et al.Evaluation of defatted black soldier fly (Hermetia illucens L.) larvae meal as an alternative protein ingredient for juvenile Japanese seabass (Lateolabrax japonicus) diets[J].Aquaculture,2019,507:144-154.
[17] 黄文庆,黄燕华,米海峰,等.3种动物蛋白质源替代鱼粉对草鱼生长性能、肌肉营养成分、消化酶活性、血清生化和抗氧化指标的影响[J].动物营养学报,2019,31(5):2187-2200.
[18] 陈晓瑛,胡俊茹,王国霞,等.黑水虻幼虫粉替代鱼粉对黄颡鱼幼鱼生长性能、肌肉品质及血清生化指标的影响[J].动物营养学报,2019,31(6):2788-2799.
[19] LI S L,JI H,ZHANG B X,et al.Defatted black soldier fly (Hermetia illucens) larvae meal in diets for juvenile Jian carp (Cyprinus carpio var.Jian):growth performance,antioxidant enzyme activities,digestive enzyme activities,intestine and hepatopancreas histological structure[J].Aquaculture,2017,477:62-70.
[20] LOCK E R,ARSIWALLA T,WAAGBØ R.Insect larvae meal as an alternative source of nutrients in the diet of Atlantic salmon (Salmo salar) postsmolt[J].Aquaculture Nutrition,2016,22(6):1202-1213.  
[21] XIAO X P,JIN P,ZHENG L Y,et al.Effects of black soldier fly (Hermetia illucens) larvae meal protein as a fishmeal replacement on the growth and immune index of yellow catfish (Pelteobagrus fulvidraco)[J].Aquaculture Research,2018,49(4):1569-1577.  
[22] KATYA K,BORSRA M Z S,GANESAN D,et al.Efficacy of insect larval meal to replace fish meal in juvenile barramundi,Lates calcarifer reared in freshwater[J].International Aquatic Research,2017,9(4):303-312.  
[23] SHUMO M.An analysis of the nutritive value of the black soldier fly larvae reared on different substrates[C]//Proceedings of the 2nd International Conference "Insects to Feed the World".Wuhan:[s.n.],2018.
[24] SPRANGHERS T,OTTOBONI M,KLOOTWIJK C,et al.Nutritional composition of black soldier fly (Hermetia illucens) prepupae reared on different organic waste substrates[J].Journal of the Science of Food and Agriculture,2017,97(8):2594-2600.  
[25] 周玉,郭文场,杨振国,等.鱼类血液学指标研究的进展[J].上海水产大学学报,2001,10(2):163-165.
[26] 朱心玲,贾丽珠,张明瑛.草鱼血液学的研究Ⅰ.九项血液常数的周年变化[J].水生生物学报,1985,9(3):248-257.
[27] 曹俊明,严晶,王国霞,等.家蝇蛆粉替代鱼粉对凡纳滨对虾消化酶、转氨酶活性和肝胰腺组织结构的影响[J].南方水产科学,2012,8(5):72-79.
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