动物营养学报 >

2019 , Vol. 31 >Issue 11: 5367 - 5377

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.11.054

研究简报 Short communications

山羊急性瘤胃酸中毒前后瘤胃菌群结构与组成变化分析

  • 李小玉 ,
  • 苏思毅 ,
  • 钟富超 ,
  • 黄莹莹 ,
  • 刘蕾红 ,
  • 李稳稳 ,
  • 林家辉 ,
  • 李锦春 ,
  • 刘亚 ,
  • 冯士彬 ,
  • 王希春 ,
  • 吴金节 ,
  • 孙裴 ,
  • 李玉
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  • 安徽农业大学动物科技学院, 合肥 230036
李小玉(1997-),女,安徽太和人,本科生,动物科学专业。E-mail:1537371098@qq.com

收稿日期: 2019-05-10

  网络出版日期: 2019-11-19

基金资助

国家重点研发计划（2018YFD0502006）；国家自然科学基金项目（31873029）；安徽省重点研究与开发计划项目（1804g07020187）；安徽省现代农业牛羊产业技术体系（AHCYJSTX-07）；安徽农业大学2018年校级质量工程项目（2018aujyxm023）

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Analysis on Structure and Composition of Rumen Bacterial Flora before and after Acute Rumen Acidosis in Goats

  • LI Xiaoyu ,
  • SU Siyi ,
  • ZHONG Fuchao ,
  • HUANG Yingying ,
  • LIU Leihong ,
  • LI Wenwen ,
  • LIN Jiahui ,
  • LI Jinchun ,
  • LIU Ya ,
  • FENG Shibin ,
  • WANG Xichun ,
  • WU Jinjie ,
  • SUN Pei ,
  • LI Yu
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  • College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China

Received date: 2019-05-10

  Online published: 2019-11-19

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摘要

瘤胃微生态平衡是山羊健康的重要保障，本试验旨在利用16S rRNA测序技术，分析山羊急性瘤胃酸中毒前后瘤胃菌群结构与组成的变化。选取健康波尔山羊4只，采用自身前后对照试验设计，将造模前采集的4份瘤胃液设为健康组，造模后采集的4份瘤胃液设为瘤胃酸中毒组。通过灌服玉米面构建山羊急性瘤胃酸中毒模型，以40 g/kg BW的比例确定玉米面用量。瘤胃液的采集和玉米面灌服均采用瘤胃插管的方法进行。结果表明：在山羊发生急性瘤胃酸中毒后，瘤胃菌群多样性和相似性降低；厚壁菌门（Firmicutes）下的乳酸杆菌目（Lactobacillales）、链球菌属（Streptococcus）成为优势菌群，变形菌门（Proteobacteria）下的琥珀酸弧菌科、Mitochondria适度生长，拟杆菌门（Bacteroidetes）下的普雷沃氏菌属（Prevotella）的相对丰度未发生显著变化（P>0.05）；LEfSe分析发现纤维杆菌门（Fibrobacteres）下的纤维杆菌科（Fibrobacteraceae）可能是造成山羊急性瘤胃酸中毒前后瘤胃菌群结构差异的影响因子之一；PICRUSt分析发现在蛋白质合成、加工与降解等功能受抑制的情况下，瘤胃菌群可能通过增加碳水化合物和氨基酸的利用，维持正常生存与繁殖。由此可见，山羊在发生急性瘤胃酸中毒后，瘤胃菌群的组成、结构与功能相较于健康状态时有较大差异。

关键词： 山羊; 急性瘤胃酸中毒; 瘤胃菌群; 16S rRNA

本文引用格式

李小玉 , 苏思毅 , 钟富超 , 黄莹莹 , 刘蕾红 , 李稳稳 , 林家辉 , 李锦春 , 刘亚 , 冯士彬 , 王希春 , 吴金节 , 孙裴 , 李玉 . 山羊急性瘤胃酸中毒前后瘤胃菌群结构与组成变化分析[J]. 动物营养学报, 2019 , 31(11) : 5367 -5377 . DOI: 10.3969/j.issn.1006-267x.2019.11.054

Abstract

Rumen microecological balance is an important guarantee for goat health. This study aimed to analyze the changes of rumen bacterial flora before and after acute rumen acidosis in goats by using 16S rRNA sequencing technology. Four healthy Boer goats were selected, and the self-designed before-after control experiment design was adopted. Four portions of tumor-producing fluid collected before modeling were set as healthy group, and four portions of tumor-producing fluid collected after modeling were set as rumen acidosis group. The goat acute rumen acidosis model was established by infusing corn flour, and the amount of corn flour was determined by the ratio of 40 g/kg BW. The method of rumen intubation was used for the collection of gastric juice and corn flour irrigation. The results showed that the diversity and similarity of rumen bacteria flora decreased after acute rumen acidosis. Streptococcus and Lactobacillales under Firmicutes became the dominant bacteria flora, Succinivibrionaceae and Mitochondria under Proteobacteria grew moderately, Prevotella under Bacteroidetes showed no significant difference in relative abundance after acute rumen acidosis (P>0.05). LEfSe analysis showed that Fibrobacteraceae under Fibrobacteres became one of the influencing factors of the difference of rumen bacterial flora composition before and after acute rumen acidosis in goats. PICRUSt analysis found that the rumen bacteria flora may maintain normal survival and reproduction by increasing the utilization of carbohydrates and amino acids when the functions of protein synthesis, processing and degradation were inhibited. It can be seen that the composition, structure and function of rumen bacteria flora have obvious changes compared with the healthy state after the induced acute rumen acidosis in goats.

Key words： goat; acute rumen acidosis; rumen bacterial flora; 16S rRNA

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