猪营养与饲料 SWINE NUTRITION AND FEED

甘草次酸对青年母猪生长性能、抗氧化能力及生殖激素浓度的影响

  • 车丽涛 ,
  • 张春勇 ,
  • 韩佃刚 ,
  • 仲崇华 ,
  • 刘水灵 ,
  • 安清聪 ,
  • 郭荣富
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  • 云南农业大学, 云南省动物营养与饲料重点实验室, 昆明 650201
车丽涛(1979-),女,云南昆明人,博士研究生,从事猪的营养与免疫基础研究。E-mail:272320726@qq.com

收稿日期: 2019-09-23

  网络出版日期: 2020-04-16

基金资助

云南省科技厅重大科技专项基金"种猪生产营养饲料技术集成与示范"资助(2012ZAO18-3)

Effects of Glycyrrhetinic Acid on Growth Performance, Antioxidant Capacity and Reproductive Hormone Concentrations of Prepubertal Gilts

  • CHE Litao ,
  • ZHANG Chunyong ,
  • HAN Diangang ,
  • ZHONG Chonghua ,
  • LIU Shuiling ,
  • AN Qingcong ,
  • GUO Rongfu
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  • Key Laboratory of Animal Nutrition and Feed Science of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China

Received date: 2019-09-23

  Online published: 2020-04-16

Supported by

 

摘要

本试验旨在研究甘草次酸对青年母猪生长性能、抗氧化能力及生殖激素浓度的影响。选用(124±7)日龄、平均体重为(61.00±3.03)kg的健康长×大二元青年母猪64头,按日龄、体重相近的原则随机分为4个组,分别为对照组(基础饲粮)、玉米赤霉烯酮组(基础饲粮+1 mg/kg玉米赤霉烯酮)、甘草次酸组(基础饲粮+400 mg/kg甘草次酸),玉米赤霉烯酮+甘草次酸组(基础饲粮+1 mg/kg玉米赤霉烯酮+400 mg/kg甘草次酸),每组4个重复,每个重复4头母猪。预试期5 d,正试期28 d。结果表明:1)与对照组相比,玉米赤霉烯酮组青年母猪阴户发育异常,阴户长、宽及面积极显著增加(P<0.01)。2)甘草次酸组和玉米赤霉烯酮+甘草次酸组青年母猪的平均日采食量和平均日增重均显著高于对照组和玉米赤霉烯酮组(P<0.05)。3)与对照组相比,玉米赤霉烯酮组青年母猪的粗脂肪消化率显著增加(P<0.05),钙和磷消化率显著降低(P<0.05);甘草次酸组的粗纤维和粗脂肪消化率显著增加(P<0.05),钙和磷消化率极显著增加(P<0.01);玉米赤霉烯酮+甘草次酸组的粗脂肪消化率显著增加(P<0.05)。4)与对照组相比,玉米赤霉烯酮组青年母猪的血清吻素(Kp)、雌二醇(E2)、促黄体素(LH)浓度极显著增加(P<0.01),甘草次酸组的血清Kp和E2浓度显著增加(P<0.05),玉米赤霉烯酮+甘草次酸组的血清Kp、E2和LH浓度极显著增加(P<0.01)。5)玉米赤霉烯酮组青年母猪的血清丙二醛(MDA)含量显著高于对照组和甘草次酸组(P<0.05);与玉米赤霉烯酮组相比,玉米赤霉烯酮+甘草次酸组的血清MDA含量显著降低(P<0.05),血清乳酸脱氢酶(LDH)活性极显著降低(P<0.01)。综上所述,饲粮中添加甘草次酸可改善青年母猪的生长性能和抗氧化能力,影响生殖激素浓度,具有缓解玉米赤霉烯酮对青年母猪繁殖危害的作用。

本文引用格式

车丽涛 , 张春勇 , 韩佃刚 , 仲崇华 , 刘水灵 , 安清聪 , 郭荣富 . 甘草次酸对青年母猪生长性能、抗氧化能力及生殖激素浓度的影响[J]. 动物营养学报, 2020 , 32(4) : 1586 -1594 . DOI: 10.3969/j.issn.1006-267x.2020.04.016

Abstract

This experiment was conducted to investigate the effects of glycyrrhetinic acid on growth performance, antioxidant capacity and reproductive hormone concentrations of prepubertal gilts. Sixty four healthy Landrace×Yorkshire prepubertal gilts at (124±7) days of age with average body weight of (61.00±3.03) kg were randomly divided into 4 groups with 4 replicates per group and 4 gilts per replicate by similar age and body weight. The 4 groups were control group (basal diet), zearalenone group (basal diet+1 mg/kg zearalenone), glycyrrhetinic acid group (basal diet+400 mg/kg glycyrrhetinic acid) and zearalenone+glycyrrhetinic acid group (basal diet+1 mg/kg zearalenone+400 mg/kg glycyrrhetinic acid). The pretest lasted for 5 days, and the test lasted for 28 days. The results showed as follows:1) compared with control group, vulva of prepubertal gilts in zearalenone group was developed abnormal and vulva length, width and area were extremely significantly increased (P<0.01). 2) Average daily feed intake and average daily gain of prepubertal gilts in glycyrrhetinic acid and zearalenone+glycyrrhetinic acid groups were significantly higher than those in control and zearalenone groups (P<0.05). 3) Compared with control group, ether extract digestibility of prepubertal gilts in zearalenone group was significantly increased (P<0.05) and calcium and phosphorus digestibilities were significantly decreased (P<0.05), crude fiber and ether extract digestibilities in glycyrrhetinic acid group were significantly increased (P<0.05) and calcium and phosphorus digestibilities were extremely significantly increased (P<0.01), and ether extract digestibility in zearalenone+glycyrrhetinic acid group was significantly increased (P<0.05). 4) Compared with control group, serum kisspeptin (Kp), estradiol (E2) and luteinizing hormone (LH) concentrations of prepubertal gilts in zearalenone group were extremely significantly increased (P<0.01), serum Kp and E2 concentrations in glycyrrhetinic acid group were significantly increased (P<0.05), and serum Kp, E2 and LH concentrations in zearalenone+glycyrrhetinic acid group were extremely significantly increased (P<0.01). 5) Serum malondialdehyde (MDA) content of prepubertal gilts in zearalenone group was significantly higher than that in control and glycyrrhetinic acid group (P<0.05). Compared with zearalenone group, serum MDA content in zearalenone+glycyrrhetinic acid group was significantly decreased (P<0.05), and serum lactate dehydrogenase (LDH) activity was extremely significantly decreased (P<0.01). In conclusion, dietary glycyrrhetinic acid can improve growth performance and antioxidant capacity of prepubertal gilts, affect reproductive hormone concentrations, and alleviate reproductive harm of zearalenone to prepubertal gilts.

参考文献

[1] MARROQUÍN-CARDONA A G,JOHNSON N M,PHILLIPS T D,et al.Mycotoxins in a changing global environment-a review[J].Food and Chemical Toxicology,2014,69:220-230.
[2] ZHANG G L,SONG J L,ZHOU Y,et al.Differentiation of sow and mouse ovarian granulosa cells exposed to zearalenone in vitro using RNA-seq gene expression[J].Toxicology and Applied Pharmacology,2018,350:78-90.
[3] SU Y,SUN Y C,JU D X,et al.The detoxification effect of vitamin C on zearalenone toxicity in piglets[J].Ecotoxicology and Environmental Safety,2018,158:284-292.
[4] HEINZI I.消除猪日粮中玉米赤霉烯酮的污染[J].何晓芳译.国外畜牧学(猪与禽),2013,33(1):17-18.
[5] 王定发.玉米赤霉烯酮联合大豆异黄酮对青年母猪生殖器官发育、肝脏损伤和组织玉米赤霉烯酮残留的影响及其机制研究[D].博士学位论文.武汉:华中农业大学,2011:9-11.
[6] CIEPLIŃSKA K,GAJECKA M,NOWAK A,et al.The genotoxicity of caecal water in gilts exposed to low doses of zearalenone[J].Toxins,2018,10(9):350.
[7] 黄珂,温晓鹿,王丽,等.霉菌毒素对畜禽的危害及削减方法研究进展[J].中国畜牧兽医,2019,46(9):2600-2607.
[8] RADWAN M O,ISMAIL M A H,EL-MEKKAWY S,et al.Synthesis and biological activity of new 18β-glycyrrhetinic acid derivatives[J].Arabian Journal of Chemistry,2016,9(3):390-399.  
[9] 智信,陈晓,苏佳灿.甘草次酸药理作用研究进展[J].现代中西医结合杂志,2019(25):2847-2850.
[10] WANG D F,ZHANG N Y,PENG Y Z,et al.Interaction of zearalenone and soybean isoflavone on the development of reproductive organs,reproductive hormones and estrogen receptor expression in prepubertal gilts[J].Animal Reproduction Science,2010,122(3/4):317-323.
[11] ZHOU M,YANG L J,CHEN Y H,et al.Comparative study of stress response,growth and development of uteri in post-weaning gilts challenged with zearalenone and estradiol benzoate[J].Animal Physiology and Animal Nutrition,2019,103(6):1885-1894.  
[12] MALEKINEJAD H,MIRZAKHANI N,RAZI M,et al.Protective effects of melatonin and Glycyrrhiza glabra extract on ochratoxin A-induced damages on testes in mature rats[J].Human Experimental Toxicology,2011,32(2):110-123.
[13] ŠPERANDA M,LIKER B,ŠPERANDA T,et al.Haematological and biochemical parameters of weaned piglets fed on fodder mixture contaminated by zearalenone with addition of clinoptilolite[J].Acta Veterinaria,2006,56(2/3):121-136.
[14] 赵虎,杨在宾,杨维仁,等.玉米赤霉烯酮对仔猪生产性能和内脏器官发育影响的研究[J].粮食与饲料工业,2008(10):37-38.
[15] 何子双,印遇龙,胡元亮,等.甘草次酸对人工乳饲养仔猪生长及血浆激素、氨基酸水平和血液学指标的影响[J].南京农业大学学报,2008,31(2):111-115.
[16] 罗宗刚,王玲,杨远新,等.甘草药渣对生长肥育猪生长性能、胴体性状和肉品质的影响[J].动物营养学报,2019,31(5):2397-2404.
[17] 冷波.玉米赤霉烯酮对断奶仔猪小肠ghrelin、PCNAHsp70表达的影响[D].硕士学位论文.泰安:山东农业大学,2016:8-16.
[18] 张明发,沈雅琴.甘草消化系统药理研究进展[J].上海医药,2009,30(6):264-267.
[19] 蔡东森.甘草次酸对团头鲂生长、抗氧化能力及品质的影响[D].硕士学位论文.南京:南京农业大学,2015:34-50.
[20] CAO Y B,LI Z P,JIANG W Y,et al.Reproductive functions of kisspeptin/KISS1R systems in the periphery[J].Reproductive Biology and Endocrinology,2019,17:65.
[21] 石玉华,陈子江.雌激素的生理意义[J].首都医科大学学报,2013,34(4):530-534.
[22] 高利华,马国辅,夏远方,等.小梅山猪性发育过程中生殖激素的变化规律[J].江苏农业科学,2013,41(9):176-178.
[23] SANTOS R R,SCHOEVERS E J,ROELEN B A,et al.Mycotoxins and female reproduction:in vitro approaches[J].World Mycotoxin Journal,2013,6(3):245-253.  
[24] ZHENG W L,FENG N F,WANG Y,et al.Effects of zearalenone and its derivatives on the synthesis and secretion of mammalian sex steroid hormones:a review[J].Food and Chemical Toxicology,2019,126:262-276.
[25] 周双海,陈清明,李振宽,等.天津白母猪生殖器官发育和生殖激素变化规律[J].北京农学院学报,2001,16(3):38-42.
[26] 刘秀凤.玉米赤霉烯酮对断奶仔猪卵巢发育的影响[D].硕士学位论文.泰安:山东农业大学,2018:10-15.
[27] 杨锦南,朱明.甘草次酸及其衍生物药理作用研究进展[J].中国药理学报,1997,13(2):110-114.
[28] MINERVINI F,DELL'AQUILA M E.Zearalenone and reproductive function in farm animals[J].International Journal of Molecular Sciences,2008,9(12):2570-2584.  
[29] 李建民,杨柳,王业秋,等.甘草次酸和甘草甜素雌激素样作用研究[J].中国预防医学杂志,2017,18(3):168-172.
[30] 闫昭明,陈清华,陈凤鸣.玉米赤霉烯酮毒性研究[J].动物营养学报,2018,30(9):3453-3458.
[31] 贺建荣,张琰,程建峰,等.黄芪总黄酮、黄芪多糖、甘草次酸及阿魏酸清除氧自由基作用的研究[J].中国美容医学,2001(3):191-193.
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