动物营养学报 >

2020 , Vol. 32 >Issue 4: 1778 - 1787

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.04.036

水产动物营养与饲料 AQUATIC ANIMAL NUTRITION AND FEED

小球藻醇提物对瓦氏黄颡鱼幼鱼生长性能、体成分、消化酶活性及血清生化和抗氧化指标的影响

  • 黄文庆 ,
  • 孙育平 ,
  • 王国霞 ,
  • 王绥涛 ,
  • 莫文艳 ,
  • 陈晓瑛 ,
  • 张永亮 ,
  • 黄燕华 ,
  • 廖宇航
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  • 1. 广东省农业科学院动物科学研究所, 农业部华南动物营养与饲料重点实验室, 广东省畜禽育种与营养研究重点实验室, 广州 510640;
    2. 华南农业大学动物科学学院, 广州 510640;
    3. 广州飞禧特生物科技有限公司, 广州 510640;
    4. 仲恺农业工程学院, 广州 510550
黄文庆(1985-),男,广东陆丰人,实验师,农业推广硕士,从事水产动物营养与饲料研究。E-mail:94574184@qq.com

收稿日期: 2019-10-08

  网络出版日期: 2020-04-16

基金资助

广东省海洋渔业科技与产业发展专项科技攻关与研发项目（A201401C01）；2016年省级现代农业科技创新联盟建设项目（2016LM082，2016LM083）

收起

Effects of Ethanol Extract from Chlorella vulgaris on Growth Performance, Body Composition, Digestive Enzyme Activities and Serum Biochemical and Antioxidant Indices of Juvenile Pelteobagrus vachelli

  • HUANG Wenqing ,
  • SUN Yuping ,
  • WANG Guoxia ,
  • WANG Suitao ,
  • MO Wenyan ,
  • CHEN Xiaoying ,
  • ZHANG Yongliang ,
  • HUANG Yanhua ,
  • LIAO Yuhang
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  • 1. Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Key Laboratory of Animal Nutrition and Feed Science in South China of Ministry of Agriculture, Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China;
    2. College of Animal Science, South China Agricultural University, Guangzhou 510640, China;
    3. Guangzhou Fishtech Biological Technology Co. Ltd., Guangzhou 510640, China;
    4. Zhongkai University of Agriculture and Engineering, Guangzhou 510550, China

Received date: 2019-10-08

  Online published: 2020-04-16

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摘要

本试验旨在研究饲料中添加不同水平的小球藻醇提物（EEFCV）对瓦氏黄颡鱼幼鱼生长性能、体成分、消化酶活性及血清生化和抗氧化指标的影响。试验选用体质量约1.85 g的瓦氏黄颡鱼苗540尾，随机分为6个组，每组3个重复，每个重复30尾鱼。各组分别投喂添加0（T0组）、0.25%（T1组）、0.50%（T2组）、1.00%（T3组）、1.50%（T4组）和2.50%（T5组）EEFCV的6种等氮等脂试验饲料，饲养周期为56 d。结果表明：1）T4和T5组的末均重、增重率和特定生长率均显著高于T0组（P<0.05），T4和T5组的饲料系数均显著低于T0组（P<0.05）。各组的存活率、肥满度、脏体比和肝体比无显著差异（P>0.05）。2）各组的粗蛋白质和粗灰分含量无显著差异（P>0.05）。T5组的粗脂肪含量显著高于T0组（P<0.05），T5组的水分含量显著低于T0组（P<0.05）。3）各组的胃、肠和肝脏的蛋白酶、淀粉酶和脂肪酶活性均无显著差异（P>0.05）。4）各组的血清谷丙转氨酶、谷草转氨酶活性及胆固醇、甘油三脂、尿素氮、葡萄糖和总蛋白含量均无显著差异（P>0.05）。T1、T3和T4组的血清谷草转氨酶活性显著高于T0组（P<0.05）。5）T4组的血清丙二醛含量显著低于T0组（P<0.05），T3、T4和T5组的血清超氧化物歧化酶活性显著高于T0组（P<0.05）。各组的血清总抗氧化能力无显著差异（P>0.05）。由此可见，饲料中添加EEFCV对黄颡鱼幼鱼生长性能和抗氧化能力有促进作用，以增重率、特定生长率和饲料系数作为主要评估指标，饲料中添加1.50%的EEFCV应用效果较佳。

关键词： 黄颡鱼; 小球藻; 生长性能; 体成分; 生化指标

本文引用格式

黄文庆 , 孙育平 , 王国霞 , 王绥涛 , 莫文艳 , 陈晓瑛 , 张永亮 , 黄燕华 , 廖宇航 . 小球藻醇提物对瓦氏黄颡鱼幼鱼生长性能、体成分、消化酶活性及血清生化和抗氧化指标的影响[J]. 动物营养学报, 2020 , 32(4) : 1778 -1787 . DOI: 10.3969/j.issn.1006-267x.2020.04.036

Abstract

This experiment was conducted to study the effects of dietary different supplemental levels of ethanol extract from Chlorella vulgaris (EEFCV) on growth performance, body composition, digestive enzymes activities and serum biochemical and antioxidant indices of juvenile Pelteobagrus vachelli. A total of 540 juvenile Pelteobagrus vachelli with initial body weight of 1.85 g were randomly divided into 6 groups with 3 replicates per group and 30 fish per replicate, and fed six isonitrogenous and isolipidic diets supplemented with 0 (T0 group), 0.25% (T1 group), 0.50% (T2 group), 1.00% (T3 group), 1.50% (T4 group) and 2.50% (T5 group) EEFCV, respectively. The feeding trail lasted for 56 days. The results showed as follows:1) the final average weight, weight gain rate and specific growth rate of T4 and T5 groups were significantly higher than those of T0 group (P<0.05), and the feed coefficient of T4 and T5 groups was significantly lower than that of T0 group (P<0.05). There were no significant differences in the survival rate, condition factor, viscerosomatic index and hepatosomatic index among all groups (P>0.05). There were no significant differences in the contents of crude protein and ash among all groups (P>0.05). The ether extract content of T5 group was significantly higher than that of T0 group (P<0.05), and the moisture content of T5 group was significantly lower than that of T0 group (P<0.05). 3) There were no significant differences in the activities of protease, amylase and lipase in stomach, intestine and liver among all groups (P>0.05). 4) There were no significant differences in the alanine transaminase and aspartate aminotransferase activities and cholesterol, triglyceride, urea nitrogen, glucose and total protein contents in serum among all groups (P>0.05). The serum aspartate aminotransferase activity of T1, T3 and T4 groups was significantly higher than that of T0 group (P<0.05). 5) The serum malondialdehyde content of T4 groups was significantly lower than that of T0 group (P<0.05), and the serum superoxide dismutase activity of T3, T4 and T5 group was significantly higher than that of T0 group (P<0.05). There was no significant difference in the serum total antioxidant capacity among all groups (P>0.05). In conclusion, dietary EEFCV supplementation has promoting effects on the growth performance and antioxidant capacity of juvenile Pelteobagrus vachelli, using weight gain rate, specific growth rate and feed coefficient as the main evaluation indices, dietary supplemented with 1.50% EEFCV has better application effects.

Key words： Pelteobagrus vachelli; Chlorella vulgaris; growth performance; body composition; biochemical indices

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