动物营养学报 >

2020 , Vol. 32 >Issue 4: 1817 - 1825

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.04.040

水产动物营养与饲料 AQUATIC ANIMAL NUTRITION AND FEED

桑叶黄酮对凡纳滨对虾肠道黏膜形态和肠道菌群的影响

  • 王咏梅 ,
  • 陈冰 ,
  • 曹俊明 ,
  • 黄燕华 ,
  • 王国霞 ,
  • 彭凯 ,
  • 莫文艳 ,
  • 赵红霞
展开
  • 1. 上海海洋大学水产与生命学院, 上海 201306;
    2. 广东省农业科学院动物科学研究所, 广州 510640;
    3. 农业部华南动物营养与饲料重点实验室, 广州 510640;
    4. 广东省动物育种与营养公共实验室, 广州 510640;
    5. 广东省畜禽育种与营养研究重点实验室, 广州 510640
王咏梅(1995-),女,四川剑阁人,硕士研究生,从事水产动物营养与饲料研究。E-mail:1361348164@qq.com

收稿日期: 2019-10-22

  网络出版日期: 2020-04-16

基金资助

2018年广东省级现代农业产业技术推广体系建设项目（2018LM1083）；广州市科技计划项目（201707010355）；广东省农业科学院水产营养与饲料学科团队项目

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Effects of Mulberry Leaf Flavonoids on Intestinal Mucosal Morphology and Intestinal Flora of Litopenaeus vannamei

  • WANG Yongmei ,
  • CHEN Bing ,
  • CAO Junming ,
  • HUANG Yanhua ,
  • WANG Guoxia ,
  • PENG Kai ,
  • MO Wenyan ,
  • ZHAO Hongxia
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  • 1. College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China;
    2. Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
    3. Key Laboratory of Animal Nutrition and Feed Science in South China of Ministry of Agriculture, Guangzhou 510640, China;
    4. Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China;
    5. Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China

Received date: 2019-10-22

  Online published: 2020-04-16

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摘要

本试验旨在研究桑叶黄酮对凡纳滨对虾（Litopenaeus vannamei）肠道黏膜形态和肠道菌群的影响。选用初始体重为（1.32±0.01）g的凡纳滨对虾800尾，随机分为5组（每组4个重复，每个重复40尾），分别饲喂在基础饲料中添加0、50、100、150和300 mg/kg桑叶黄酮的试验饲料。饲养周期为50 d。结果显示：1）饲料中添加桑叶黄酮可促进凡纳滨对虾肠道肠绒毛发育，增加肌层厚度，添加100 mg/kg桑叶黄酮组绒毛高度最高且显著高于其他组（P<0.05），添加50 mg/kg桑叶黄酮组肌层厚度最高且显著高于其他组（P<0.05）。2）饲料中添加桑叶黄酮可增加凡纳滨对虾肠道菌群多样性，添加50 mg/kg桑叶黄酮组Shannon指数显著高于未添加组（P<0.05），Simpson指数显著低于未添加组（P<0.05）。3）与未添加组相比，添加100和150 mg/kg桑叶黄酮组弧菌属（Vibrio）与添加50、100和150 mg/kg桑叶黄酮组希瓦氏菌属（Shewanella）的相对丰度显著增加（P<0.05），各桑叶黄酮添加组红球菌属（Rhodococcus）与添加100、150和300 mg/kg桑叶黄酮组乳球菌属（Lactococcus）的相对丰度显著降低（P<0.05）。由此得出，饲料中添加50 mg/kg桑叶黄酮可促进凡纳滨对虾肠道发育，增加肠道菌群多样性。

关键词： 桑叶黄酮; 凡纳滨对虾; 肠道黏膜形态; 肠道菌群

本文引用格式

王咏梅 , 陈冰 , 曹俊明 , 黄燕华 , 王国霞 , 彭凯 , 莫文艳 , 赵红霞 . 桑叶黄酮对凡纳滨对虾肠道黏膜形态和肠道菌群的影响[J]. 动物营养学报, 2020 , 32(4) : 1817 -1825 . DOI: 10.3969/j.issn.1006-267x.2020.04.040

Abstract

The effects of mulberry leaf flavonoids on intestinal mucosal morphology and intestinal flora of Litopenaeus vannamei were evaluated in this study. A total of 800 Litopenaeus vannamei with an initial body weight of (1.32±0.01) g were divided into 5 groups with 4 replicates in each group and 40 shrimps in each replicate. Four diets were formulated to contain 0, 50, 100, 150 and 300 mg/kg diet of mulberry leaf flavonoids. The trial lasted for 50 days. The results showed as follows:1) dietary mulberry leaf flavonoids could improve the villus development and increase the muscle layer. thickness of intestine of shrimp. The height of villus in the 100 mg/kg group was the highest and significantly higher than that of other groups (P<0.05), and the thickness of muscle layer in the 50 mg/kg group was the highest and higher significantly than that of other groups (P<0.05). 2) Dietary mulberry leaf flavonoids could increase the intestinal flora diversity of shrimp. The Shannon index was significantly increased and the Simpson index was significantly decreased in the 50 mg/kg group as compared with 0 mg/kg group (P<0.05). 3) Compared with the no added group, adding mulberry leaf flavonoids into the diet could significantly increase the relative abundances of Vibrio (100 and 150 mg/kg) and Shewanella (50, 100 and 150 mg/kg) (P<0.05), whereas significantly decrease the relative abundances of Lactococcus (100, 150 and 300 mg/kg) and Rhodococcus (50, 100, 150 and 300 mg/kg) (P<0.05). In conclusion, adding 50 mg/kg of mulberry leaf flavonoids into the diet can promote the intestinal development and increase the intestinal flora diversity of Litopenaeus vannamei.

Key words： mulberry leaf flavonoids; Litopenaeus vannamei; intestinal mucosal morphology; intestinal flora

参考文献

[1] RIOS-COVIAN D,SALAZAR N,GUEIMONDE M,et al.Shaping the metabolism of intestinal Bacteroides population through diet to improve human health[J].Frontiers in Microbiology,2017,8:376.
[2] REN D Y,GONG S J,SHU J Y,et al.Mixed Lactobacillus plantarum strains inhibit Staphylococcus aureus induced inflammation and ameliorate intestinal microflora in mice[J].BioMed Research International,2017(2017):1-7.
[3] ADEL M,YEGANEH S,DAWOOD M A O,et al.Effects of Pediococcus pentosaceus supplementation on growth performance,intestinal microflora and disease resistance of white shrimp,Litopenaeus vannamei[J].Aquaculture Nutrition,2017,23(3):1401-1409.
[4] 胡晓伟,上官静波,黎中宝,等.低聚木糖对花鲈幼鱼生长性能、血清生化和免疫指标及肠道菌群组成的影响[J].动物营养学报,2018,30(2):734-742.
[5] GILBERT J A,BLASER M J,CAPORASO J G,et al.Current understanding of the human microbiome[J].Nature Medicine,2018,24(4):392-400.  
[6] TZUC J T,ESCALANTE D R,HERRERA R R,et al.Microbiota from Litopenaeus vannamei:digestive tract microbial community of Pacific white shrimp (Litopenaeus vannamei)[J].SpringerPlus,2014,3:280.
[7] 何佳珂,于洋,陈西敬,等.黄酮类化合物的药物代谢研究进展[J].中国中药杂志,2010,35(21):2789-2794.
[8] 杨继华,陈冰,黄燕华,等.桑叶黄酮对吉富罗非鱼生长性能及抗氧化能力的影响[C].侯永清.中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会论文集.北京:中国农业大学出版社,2016:633.
[9] 陈冰,杨继华,曹俊明,等.桑叶黄酮对吉富罗非鱼肌肉抗氧化指标及营养组成的影响[J].淡水渔业,2018,48(3):90-95.
[10] 杭汉强,陈香花.肠道微生物与黄酮类化合物的生物转化[J].中国现代实用医学杂志,2006,5(3):30-34.
[11] 陈玉慧,郭彤彤,张鑫,等.青钱柳黄酮及三萜调节人体肠道菌群作用研究[J].中国野生植物资源,2019,38(2):6-10,15.
[12] 刘敏,陈奇超,胡瑞良,等.藤三七类黄酮组分对小鼠代谢综合征模型及其肠道菌群的调节作用[J].中国微生态学杂志,2017,29(4):390-394.
[13] 张梅梅.黄酮类化合物抗氧化能力的比较及其对肠道菌群的调节作用[D].硕士学位论文.重庆:西南大学,2018.
[14] 郭海燕,张彦娇,麦康森,等.染料木黄酮对大菱鲆生长、消化酶活力和肠道组织结构的影响[J].中国海洋大学学报(自然科学版),2014,44(9):30-36.
[15] 张伟.大豆皂甙与大豆异黄酮对异育银鲫生长、生理及肠道健康的影响[D].苏州:苏州大学,2010.
[16] 熊建华,赵永贞,高永华,等.凡纳滨对虾良种培育研究进展[J].南方农业学报,2011,42(5):556-561.
[17] 林成招,马海田,邹思湘,等.大豆异黄酮对大鼠小肠上皮细胞生长及葡萄糖和氨基酸吸收的影响[J].南京农业大学学报,2005,28(1):71-75.
[18] 晁杰.大豆异黄酮对鸡小肠黏膜上皮细胞的影响[D].硕士学位论文.福州:福建农林大学,2016.
[19] STANGER B Z,DATAR R,MURTAUGH L C,et al.Direct regulation of intestinal fate by Notch[J].Proceedings of the National Academy of Sciences of the United States of America,2005,102(35):12443-12448.  
[20] 胡海滨,刘金桃,李彦先,等.饲料中大豆黄酮对大菱鲆生长、消化酶活力、抗氧化力及肠道结构的影响[J].水产学报,2014,38(9):1503-1513.
[21] GIACOMIN P,AGHA Z,LOUKAS A.Helminths and intestinal flora team up to improve gut health[J].Trends in Parasitology,2016,32(9):664-666.  
[22] SCHOEFER L,MOHAN R,SCHWIERTZ A,et al.Anaerobic degradation of flavonoids by Clostridium orbiscindens[J].Applied and Environmental Microbiology,2003,69(10):5849-5854.  
[23] 高志敏.白花蛇舌草总黄酮苷和苷元的制备及肠道菌群代谢研究[D].硕士学位论文.济南:山东中医药大学,2013.
[24] JONES S E,LENNON J T.Dormancy contributes to the maintenance of microbial diversity[J].Proceedings of the National Academy of Sciences of the United States of America,2010,107(13):5881-5886.  
[25] XIONG J B,WANG K,WU J F,et al.Changes in intestinal bacterial communities are closely associated with shrimp disease severity[J].Applied Microbiology and Biotechnology,2015,99(16):6911-6919.  
[26] 郁维娜,戴文芳,陶震,等.健康与患病凡纳滨对虾肠道菌群结构及功能差异研究[J].水产学报,2018,42(3):399-409.
[27] 杨硕.凡纳滨对虾肠道菌群分析及其利用[D].硕士学位论文.天津:天津科技大学,2016.
[28] 郭慧,朱旭枫,陈锦霖,等.水解单宁对凡纳滨对虾生长性能和肠道微生物的影响[J].中国水产科学,2019,26(5):81-86.
[29] 王元,周俊芳,韦信贤,等.海水和淡水养殖凡纳滨对虾肠道和鳃的菌群结构分析[J].湖南农业大学学报(自然科学版),2018,44(2):198-203.
[30] 李海兵.对虾肠道益生菌的筛选与免疫物质活性评价指标的建立[D].硕士学位论文.青岛:中国海洋大学,2008.
[31] 杨慧婷.对虾肠道菌群动态平衡的免疫调控研究[D].博士学位论文.济南:山东大学,2016.
[32] LUIS-VILLASENOR I E,CASTELLANOS-CERVANTES T,GOMEZ-GIL B,et al.Probiotics in the intestinal tract of juvenile whiteleg shrimp Litopenaeus vannamei:modulation of the bacterial community[J].World Journal of Microbiology and Biotechnology,2013,29(2):257-265.  
[33] TZUC J T,ESCALANTE D R,HERRERA R R,et al.Microbiota from Litopenaeus vannamei:digestive tract microbial community of Pacific white shrimp (Litopenaeus vannamei)[J].SpringerPlus,2014,3:280.
[34] BALCÁZAR J L,ROJAS-LUNA T,CUNNINGHAM D P.Effect of the addition of four potential probiotic strains on the survival of pacific white shrimp (Litopenaeus vannamei) following immersion challenge with Vibrio parahaemolyticus[J].Journal of Invertebrate Pathology,2007,96(2):147-150.  
[35] 徐君.黄芩-黄连药对与肠道菌群的相互作用研究[D].硕士学位论文.南京:南京中医药大学,2014.
[36] 张蔚.二氢黄酮苷及异黄酮苷与人体肠道细菌的相互作用研究[D].硕士学位论文.南京:南京中医药大学,2014.
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