饲粮添加锰对3~4月龄獭兔脂肪沉积和脂肪组织脂质代谢的影响

陈晓阳; 杨国雨; 李凡; 张斌; 李晨阳; 李福昌

doi:10.3969/j.issn.1006-267x.2020.04.042

动物营养学报 >

2020 , Vol. 32 >Issue 4: 1836 - 1841

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.04.042

特种经济动物营养与饲料 SPECIAL ECONOMIC ANIMAL NUTRITION AND FEED

饲粮添加锰对3~4月龄獭兔脂肪沉积和脂肪组织脂质代谢的影响

陈晓阳 ,
杨国雨 ,
李凡 ,
张斌 ,
李晨阳 ,
李福昌

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1. 山东农业大学动物科技学院, 泰安 271018;
2. 山东省动物生物工程与疾病防治重点实验室, 泰安 271018

陈晓阳(1994-),男,山东临沂人,硕士研究生,从事家兔营养与代谢的研究。E-mail:229658021@qq.com

收稿日期: 2019-10-17

网络出版日期: 2020-04-16

基金资助

现代农业产业技术体系建设专项（CARS-43-B-1）；山东省双一流建设项目（SYL2017YSTD11）

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Effects of Dietary Manganese on Fat Deposition and Lipid Metabolism of Fat Tissue in 3 to 4-Month-Old Rex Rabbits

CHEN Xiaoyang ,
YANG Gouyu ,
LI Fan ,
ZHANG Bin ,
LI Chenyang ,
LI Fuchang

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1. College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China;
2. Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an 271018, China

Received date: 2019-10-17

Online published: 2020-04-16

Supported by

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摘要

本试验旨在研究饲粮添加锰对生长獭兔脂肪组织脂质代谢的影响。选用初始体重相近、健康状况良好的3月龄獭兔200只，随机分为5组（每组40个重复，每个重复1只），分别饲喂在基础饲粮中添加0（对照）、5、10、20、40 mg/kg锰（以硫酸锰的形式）的试验饲粮。预试期7 d，正试期29 d。结果表明：1）与对照组相比，饲粮中添加10~20 mg/kg锰显著降低了肩胛脂肪沉积率（P<0.05），饲粮中添加5~40 mg/kg锰显著降低了胃周脂肪沉积率（P<0.05）。饲粮中锰添加水平对肾周脂肪沉积率没有显著影响（P>0.05）。2）饲粮中锰添加水平对肉碱脂酰转移酶2（CPT2）、激素敏感脂酶（HSL）和过氧化物酶体增殖物激活受体β（PPARβ）基因的表达量没有显著影响（P>0.05）。与对照组相比，饲粮中添加20~40 mg/kg锰显著降低了脂肪酸合成酶（FAS）基因的表达量（P<0.05）；饲粮中添加20~40 mg/kg锰显著降低了乙酰辅酶A羧化酶（ACC）基因的表达量（P<0.05）；饲粮中添加10~40 mg/kg锰显著提高了肉碱脂酰转移酶1（CPT1）基因的表达量（P<0.05）；饲粮中添加10~20 mg/kg锰显著提高过氧化物酶体增殖物激活受体α（PPARα）基因的表达量（P<0.05）。综上所述，饲粮中添加锰影响了3~4月龄獭兔的脂肪沉积和脂肪组织脂质代谢，锰添加水平为20 mg/kg时，可以有效促进脂肪组织的氧化分解，抑制脂肪组织的合成，并能降低体脂肪的沉积率。

关键词： 锰; 獭兔; 脂肪; 脂肪代谢; 基因表达

本文引用格式

陈晓阳 , 杨国雨 , 李凡 , 张斌 , 李晨阳 , 李福昌 . 饲粮添加锰对3~4月龄獭兔脂肪沉积和脂肪组织脂质代谢的影响[J]. 动物营养学报, 2020 , 32(4) : 1836 -1841 . DOI: 10.3969/j.issn.1006-267x.2020.04.042

Abstract

The experiment was conducted to investigate the effects of dietary manganese on lipid metabolism of fat tissue in growing Rex rabbits. Two hundred 3-month-old health Rex rabbits with similar body weight and good health were randomly divided into 5 groups with 40 replicates in each group and 1 rabbit in each replicate. Rabbits in 5 groups were fed a basal diet containing 0 (control), 5, 10, 20 and 40 mg/kg manganese (in the form of manganese sulfate), respectively. The trial lasted for 7 days for adaptation, and 29 days for test. The results showed as follows:1) compared with the control group, the addition of 10 to 20 mg/kg manganese significantly reduced the scapular fat deposition rate (P<0.05), and the addition of 5 to 40 mg/kg manganese significantly reduced the perigastric fat deposition rate (P<0.05). The level of manganese in the diet had no effect on the perirenal fat deposition rate (P>0.05). 2) The levels of manganese added to the diet had no significant effects on the expression levels of carnitine fatty acyltransferase 2 (CPT2), hormone sensitive lipase (HSL) and peroxisome proliferator-activated receptor beta (PPARβ) genes (P>0.05). Compared with the control group, the addition of 20 to 40 mg/kg manganese significantly reduced the expression level of fatty acid synthase (FAS) gene (P<0.05); the addition of 20 to 40 mg/kg manganese significantly reduced the expression of acetyl-CoA carboxylase (ACC) gene (P<0.05); the addition of 10 to 40 mg/kg manganese in the diet significantly increased the expression of carnitine fatty acyltransferase 1 (CPT1) gene (P<0.05); the addition of 10 to 20 mg/kg manganese in the diet significantly increased the expression of peroxisome proliferator-activated receptor alpha (PPARα) gene (P<0.05). From what has been discussed above, addition of manganese affects fat deposition and fat metabolism of Rex rabbits. When the level of manganese addition is 20 mg/kg, it can effectively promote the oxidation and decomposition of fat in fat tissue, inhibit fat synthesis in fat tissue, and reduce the deposition rate of body fat.

Key words： manganese; Rex rabbits; fat; fat metabolism; genetic expression

参考文献

[1] CABALLERO B,FINGLAS P M,TOLDRÁ F.Encyclopedia of food and health[M].Amsterdam:Elsevier Ltd,2016:637-640.
[2] 朱玉琴,张日俊.0-2周龄肉用仔鸡日粮中锰需要量的研究[J].动物营养学报,1997,3(3):13-22.
[3] AKSU D S,AKSU T,QZSOY B.The effects of lower supplementation levels of organically complexed minerals (zinc,copper and manganese) versus inorganic forms on hematological and biochemical parameters in broilers[J].Kafkas Vniversitesi Veteriner Fakültesi Dergisi,2010,16(4):553-559.

[4] FREELAND-GRAVES J,LLANES C.Models to study manganese deficiency[M]//KLIMIS-TAVANTZIS D J.Manganese in health and disease.Boca Raton,FL:CRC Press,1994:115-120.
[5] KEEN C L,ENSUNSA J L,WATSON M H,et al.Nutritional aspects of manganese from experimental studies[J].Neurotoxicology,1999,20:213-223.
[6] LU L,JI C,LUO X G,et al.The effect of supplemental manganese in broiler diets on abdominal fat deposition and meat quality[J].Animal Feed Science and Technology,2006,129(1/2):9-59.
[7] LU L,JI C,LUO X G,et al.The effect of supplemental manganese in broiler diets on abdominal fat deposition and meat quality[J].Animal Feed Science and Technology,2006,129(1/2):49-59.
[8] DE BLAS C,MATEOS G.The nutrition of the rabbit[M].New York:CABI Publishing,1998:297-308.
[9] DE BLAS C,WISEMAN J.Nutrition of the rabbit[M].2nd ed.New York:LCABI Publishing,2010:333-342.
[10] SANDS J S,SMITH M O.Broilers in heat stress conditions:effects of dietary manganese proteinate or chromium picolinate supplementation[J].Journal of Applied Poultry Science,1999,8(3):280-287.

[11] 沈同,王镜岩,赵邦悌,等.生物化学[M].2版.北京:高等教育出版社,1990:151-167.
[12] 熊文中,杨凤,周安国.猪重组生长激素对不同杂交肥育猪脂肪代谢调控的研究[J].畜牧兽医学报,2001,32(1):1-4.
[13] MUKHERJEE S,KATIYAR S S.Nature of O-phthalaldehyde reaction with pigeon liver fatty acid synthetase[J].Indian Journal of Biochemistry & Biophysics,1999,36(2):63-68.
[14] 张光磊,冯金曼,崔明勋,等.乙酰辅酶A羧化酶抑制剂对肥胖小鼠的减肥效果[J].畜牧与饲料科学,2012,33(7):10-13.
[15] 李亮,程彦伟.乙酰辅酶A羧化酶在治疗肥胖中的潜在作用[J].生命的化学,2007,27(2):180-182.
[16] ABU-ELHEIGA L,MATZUK M M,ABO-HASHEMA K A H,et al.Continuous fatty acid oxidation and reduced fat storage in mice lacking acetyl-CoA carboxylase 2[J].Science,2001,291(5513):2613-2616.

[17] BRITTON C H,MACKEY D W,ESSER V,et al.Fine chromosome mapping of the genes for human liver and muscle carnitine palmitoyltransferase Ⅰ (CPT1A and CPT1B)[J].Genomics,1997,40(1):209-211.

[18] DEBERARDINIS R J,LUM J J,THOMPSON C B.Phosphatidylinositol 3-kinase-dependent modulation of carnitine palmitoyltransferase 1A expression regulates lipid metabolism during hematopoietic cell growth[J].Journal of Biological Chemistry,2006,281(49):37372-37380.

[19] KRAEMER F B,SHEN W J.Hormone-sensitive lipase knockouts[J].Nutrition & Metabolism,2006,3:12.
[20] SATO K,AKIBA Y,CHIDA Y,et al.Lipoprotein hydrolysis and fat accumulation in chicken adipose tissues are reduced by chronic administration of lipoprotein lipase monoclonal antibodies[J].Poultry Science,1999,78(9):1286-1291.

[21] DUGAIL I,QUIGNARD-BOULANGE A,BRIGANT L,et al.Increased lipoprotein lipase content in the adipose tissue of suckling and weaning obese zucker rats[J].The Biochemical Journal,1988,249(1):45-49.

[22] FEIGE J N,GELMAN L,MICHALIK L,et al.From molecular action to physiological outputs:peroxisome proliferator-activated receptors are nuclear receptors at the crossroads of key cellular functions[J].Progress in Lipid Research,2006,45(2):120-159.

[23] 张玥,姜宁,苏丽,等.PPARα与运动改善脂质代谢的关系[J].中国康复淤血杂志,2008,23(6):495-498,504.
[24] 丁萍萍,张陆勇.过氧化物酶体增殖物激活受体α在脂质代谢中的作用研究进展[J].临床合理用药,2019,12(4):177-179.

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